Exploring the potential of Inula viscosa extracts for antioxidant, antiproliferative and apoptotic effects on human liver cancer cells and a molecular docking study
In folk medicine, Inula viscosa (Asteraceae) has been traditionally utilized for treating various ailments, including diabetes, bronchitis, diarrhea, rheumatism, and injuries. In this study, we aimed to investigate the chemical composition, antioxidant, antiproliferative, and apoptotic properties...
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| Format: | Article |
| Language: | English |
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Termedia Publishing House
2023-06-01
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| Series: | BioTechnologia |
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| Online Access: | https://www.termedia.pl/Exploring-the-potential-of-Inula-viscosa-extracts-for-antioxidant-antiproliferative-and-apoptotic-effects-on-human-liver-cancer-cells-and-a-molecular-docking-study,85,50644,1,1.html |
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| author | Naoual Kheyar-Kraouche Saliha Boucheffa Yuva Bellik Kheyar Farida Nabila Brahmi-Chendouh |
| author_facet | Naoual Kheyar-Kraouche Saliha Boucheffa Yuva Bellik Kheyar Farida Nabila Brahmi-Chendouh |
| author_sort | Naoual Kheyar-Kraouche |
| collection | DOAJ |
| description | In folk medicine, Inula viscosa (Asteraceae) has been traditionally utilized for treating various ailments, including diabetes, bronchitis, diarrhea, rheumatism, and injuries. In this study, we aimed to investigate the chemical composition, antioxidant, antiproliferative, and apoptotic properties of I. viscosa leaf extracts. Extraction was performed using solvents of varying polarities. Antioxidant activity was determined using Ferric reducing anti¬oxidant power (FRAP) and 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. The results reve¬aled that aqueous ethanol (70%) and aqueous ethyl acetate (70%) extracts contained high levels of phenols (645.58±8.77 mg CE/g) and flavonoids (180.69±1.54 mg QE/g), respectively. Aqueous ethanol (70%) extract exhibited the highest antioxidant activity with IC50 of 572.74 μmol TE/g DW (μmol Trolox equivalent in 1g of dry extract) in the ABTS assay and 76862.06 μM TE/g DW in the FRAP test. All extracts showed a considerable dose-dependent cytotoxic effect on cancerous HepG2 cells ( P < 0.05). The aqueous ethanol extract demonstrated the highest inhibitory effect (IC50 = 1.67 mg/ml). Treatment with aqueous ethanol (70%) and pure ethyl acetate extracts significantly increased the number of apoptotic cells to 8 and 6%, respectively, in HepG2 cells ( P < 0.05). Additionally, the aqueous ethanol extract significantly elevatedreactive oxygen species (ROS) levels (53%) in HepG2 cells. The molecular docking study identified paxanthone and banaxanthone E as the compounds that exhibited the highest binding affinities with BCL-2. This study demonstrated the potent antioxidant, antiproliferation, and intracellular ROS production of I. viscosa leaf extracts. Further studies should be conducted to identify the active compounds involved. |
| first_indexed | 2024-03-12T02:27:33Z |
| format | Article |
| id | doaj.art-f7da46bf6aa644179e71aba71d1e209d |
| institution | Directory Open Access Journal |
| issn | 0860-7796 2353-9461 |
| language | English |
| last_indexed | 2024-03-12T02:27:33Z |
| publishDate | 2023-06-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | BioTechnologia |
| spelling | doaj.art-f7da46bf6aa644179e71aba71d1e209d2023-09-05T11:45:55ZengTermedia Publishing HouseBioTechnologia0860-77962353-94612023-06-01104218319810.5114/bta.2023.12720750644Exploring the potential of Inula viscosa extracts for antioxidant, antiproliferative and apoptotic effects on human liver cancer cells and a molecular docking studyNaoual Kheyar-KraoucheSaliha BoucheffaYuva BellikKheyar FaridaNabila Brahmi-ChendouhIn folk medicine, Inula viscosa (Asteraceae) has been traditionally utilized for treating various ailments, including diabetes, bronchitis, diarrhea, rheumatism, and injuries. In this study, we aimed to investigate the chemical composition, antioxidant, antiproliferative, and apoptotic properties of I. viscosa leaf extracts. Extraction was performed using solvents of varying polarities. Antioxidant activity was determined using Ferric reducing anti¬oxidant power (FRAP) and 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. The results reve¬aled that aqueous ethanol (70%) and aqueous ethyl acetate (70%) extracts contained high levels of phenols (645.58±8.77 mg CE/g) and flavonoids (180.69±1.54 mg QE/g), respectively. Aqueous ethanol (70%) extract exhibited the highest antioxidant activity with IC50 of 572.74 μmol TE/g DW (μmol Trolox equivalent in 1g of dry extract) in the ABTS assay and 76862.06 μM TE/g DW in the FRAP test. All extracts showed a considerable dose-dependent cytotoxic effect on cancerous HepG2 cells ( P < 0.05). The aqueous ethanol extract demonstrated the highest inhibitory effect (IC50 = 1.67 mg/ml). Treatment with aqueous ethanol (70%) and pure ethyl acetate extracts significantly increased the number of apoptotic cells to 8 and 6%, respectively, in HepG2 cells ( P < 0.05). Additionally, the aqueous ethanol extract significantly elevatedreactive oxygen species (ROS) levels (53%) in HepG2 cells. The molecular docking study identified paxanthone and banaxanthone E as the compounds that exhibited the highest binding affinities with BCL-2. This study demonstrated the potent antioxidant, antiproliferation, and intracellular ROS production of I. viscosa leaf extracts. Further studies should be conducted to identify the active compounds involved.https://www.termedia.pl/Exploring-the-potential-of-Inula-viscosa-extracts-for-antioxidant-antiproliferative-and-apoptotic-effects-on-human-liver-cancer-cells-and-a-molecular-docking-study,85,50644,1,1.htmlinula viscosa leaf extracts antioxidant cytotoxic effect hepg2 cells ros molecular docking |
| spellingShingle | Naoual Kheyar-Kraouche Saliha Boucheffa Yuva Bellik Kheyar Farida Nabila Brahmi-Chendouh Exploring the potential of Inula viscosa extracts for antioxidant, antiproliferative and apoptotic effects on human liver cancer cells and a molecular docking study BioTechnologia inula viscosa leaf extracts antioxidant cytotoxic effect hepg2 cells ros molecular docking |
| title | Exploring the potential of Inula viscosa extracts for antioxidant, antiproliferative and apoptotic effects on human liver cancer cells and a molecular docking study |
| title_full | Exploring the potential of Inula viscosa extracts for antioxidant, antiproliferative and apoptotic effects on human liver cancer cells and a molecular docking study |
| title_fullStr | Exploring the potential of Inula viscosa extracts for antioxidant, antiproliferative and apoptotic effects on human liver cancer cells and a molecular docking study |
| title_full_unstemmed | Exploring the potential of Inula viscosa extracts for antioxidant, antiproliferative and apoptotic effects on human liver cancer cells and a molecular docking study |
| title_short | Exploring the potential of Inula viscosa extracts for antioxidant, antiproliferative and apoptotic effects on human liver cancer cells and a molecular docking study |
| title_sort | exploring the potential of inula viscosa extracts for antioxidant antiproliferative and apoptotic effects on human liver cancer cells and a molecular docking study |
| topic | inula viscosa leaf extracts antioxidant cytotoxic effect hepg2 cells ros molecular docking |
| url | https://www.termedia.pl/Exploring-the-potential-of-Inula-viscosa-extracts-for-antioxidant-antiproliferative-and-apoptotic-effects-on-human-liver-cancer-cells-and-a-molecular-docking-study,85,50644,1,1.html |
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