Preparation of a carboxymethylated diethyl aminoethyl cellulose-collagen nanocomposite scaffold as a candidate for skin tissue engineering
Extensive wounds might result in massive infections, deformation, or organ malfunction. Conventional therapies like skin grafting possess some drawbacks, therefore, the urge of developing tissue engineering techniques and skin substitutes has emerged. During this study, a biocompatible nanoscaffold...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-07-01
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| Series: | Journal of Materials Research and Technology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S223878542200895X |
| _version_ | 1811281199937617920 |
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| author | Parand Khadivar Safoura Khajeniazi Afzal Karimi |
| author_facet | Parand Khadivar Safoura Khajeniazi Afzal Karimi |
| author_sort | Parand Khadivar |
| collection | DOAJ |
| description | Extensive wounds might result in massive infections, deformation, or organ malfunction. Conventional therapies like skin grafting possess some drawbacks, therefore, the urge of developing tissue engineering techniques and skin substitutes has emerged. During this study, a biocompatible nanoscaffold was fabricated from wood nanocellulose, collagen, and carboxymethyl diethylaminoethyl cellulose to be used in the wound regeneration process. The main chemical, physical, and biological features of the synthesized nanoscaffold were determined by employing various techniques. In addition, an in vivo assay was performed to evaluate the allergic or immunogenic properties and potential healing effects of the scaffold. Zeta potential was measured to be −43.9 mv reflecting the high stability of the scaffold. The crystallite size was determined to be 69 nm by XRD which was in the nanoparticle size range. The crystallinity index was estimated to be 66.8% indicating more crystalline nature of the nanocomposite. PH was adjusted to 5.6 to be suitable for the wound healing process. The scaffold was proved to be biocompatible and showed improved water uptake capacity and adhesive properties. Moreover, no allergic or immune response was induced while applying the scaffold to a biological system. It was proved the scaffold assists in the wound healing process by histological studies. This study shows the synthesized nanoscaffold is a good candidate to be used in the wound regeneration process. It could combine with cell therapy to increase the healing effect. There could be a possibility to use the scaffold as a biological Band-Aid following more intensive clinical trials in the future. |
| first_indexed | 2024-04-13T01:29:17Z |
| format | Article |
| id | doaj.art-f7de488f2ee84f72955f83252792fec4 |
| institution | Directory Open Access Journal |
| issn | 2238-7854 |
| language | English |
| last_indexed | 2024-04-13T01:29:17Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Materials Research and Technology |
| spelling | doaj.art-f7de488f2ee84f72955f83252792fec42022-12-22T03:08:34ZengElsevierJournal of Materials Research and Technology2238-78542022-07-011939663979Preparation of a carboxymethylated diethyl aminoethyl cellulose-collagen nanocomposite scaffold as a candidate for skin tissue engineeringParand Khadivar0Safoura Khajeniazi1Afzal Karimi2Department of Medical Biotechnology, College of Advanced Technologies in Medicine, Golestan University of Medical Sciences, Golestan, IranStem Cell Research Center, Golestan University of Medical Sciences, Golestan, Iran; Corresponding author.Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran; Corresponding author.Extensive wounds might result in massive infections, deformation, or organ malfunction. Conventional therapies like skin grafting possess some drawbacks, therefore, the urge of developing tissue engineering techniques and skin substitutes has emerged. During this study, a biocompatible nanoscaffold was fabricated from wood nanocellulose, collagen, and carboxymethyl diethylaminoethyl cellulose to be used in the wound regeneration process. The main chemical, physical, and biological features of the synthesized nanoscaffold were determined by employing various techniques. In addition, an in vivo assay was performed to evaluate the allergic or immunogenic properties and potential healing effects of the scaffold. Zeta potential was measured to be −43.9 mv reflecting the high stability of the scaffold. The crystallite size was determined to be 69 nm by XRD which was in the nanoparticle size range. The crystallinity index was estimated to be 66.8% indicating more crystalline nature of the nanocomposite. PH was adjusted to 5.6 to be suitable for the wound healing process. The scaffold was proved to be biocompatible and showed improved water uptake capacity and adhesive properties. Moreover, no allergic or immune response was induced while applying the scaffold to a biological system. It was proved the scaffold assists in the wound healing process by histological studies. This study shows the synthesized nanoscaffold is a good candidate to be used in the wound regeneration process. It could combine with cell therapy to increase the healing effect. There could be a possibility to use the scaffold as a biological Band-Aid following more intensive clinical trials in the future.http://www.sciencedirect.com/science/article/pii/S223878542200895XCarboxymethyl diethylaminoethyl celluloseCollagenNanocelluloseNanocompositeWound regeneration |
| spellingShingle | Parand Khadivar Safoura Khajeniazi Afzal Karimi Preparation of a carboxymethylated diethyl aminoethyl cellulose-collagen nanocomposite scaffold as a candidate for skin tissue engineering Journal of Materials Research and Technology Carboxymethyl diethylaminoethyl cellulose Collagen Nanocellulose Nanocomposite Wound regeneration |
| title | Preparation of a carboxymethylated diethyl aminoethyl cellulose-collagen nanocomposite scaffold as a candidate for skin tissue engineering |
| title_full | Preparation of a carboxymethylated diethyl aminoethyl cellulose-collagen nanocomposite scaffold as a candidate for skin tissue engineering |
| title_fullStr | Preparation of a carboxymethylated diethyl aminoethyl cellulose-collagen nanocomposite scaffold as a candidate for skin tissue engineering |
| title_full_unstemmed | Preparation of a carboxymethylated diethyl aminoethyl cellulose-collagen nanocomposite scaffold as a candidate for skin tissue engineering |
| title_short | Preparation of a carboxymethylated diethyl aminoethyl cellulose-collagen nanocomposite scaffold as a candidate for skin tissue engineering |
| title_sort | preparation of a carboxymethylated diethyl aminoethyl cellulose collagen nanocomposite scaffold as a candidate for skin tissue engineering |
| topic | Carboxymethyl diethylaminoethyl cellulose Collagen Nanocellulose Nanocomposite Wound regeneration |
| url | http://www.sciencedirect.com/science/article/pii/S223878542200895X |
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