Characterization of aminopeptidase encoding gene anp-1 and its association with development in Caenorhabditis elegans
Background Aminopeptidases play important roles in various biological processes in nematodes including growth, development and reproduction. Although the aminopeptidases have been shown to regulate reproduction in Caenorhabditis elegans (C. elegans), the role of aminopeptidases in development and ag...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2019-11-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/7944.pdf |
| _version_ | 1827606055478099968 |
|---|---|
| author | Shanchun Su Baoliang Pan Yanxin Hu Ming Wang |
| author_facet | Shanchun Su Baoliang Pan Yanxin Hu Ming Wang |
| author_sort | Shanchun Su |
| collection | DOAJ |
| description | Background Aminopeptidases play important roles in various biological processes in nematodes including growth, development and reproduction. Although the aminopeptidases have been shown to regulate reproduction in Caenorhabditis elegans (C. elegans), the role of aminopeptidases in development and aging has not been reported. This study focused on the function of aminopeptidase AlaNyl aminopeptidase 1 (ANP-1) on development in C. elegans. Methods In the present study, we reported the identification of ANP-1 in C. elegans along with sequence analysis and its functional expression and characterization. The phenotype changes were observed when anp-1 mutated. Then, differential expression genes (DEGs) between wild type strain (N2) and anp-1 deletion strain (RB804) were identified using transcriptome sequencing method. Finally, DEGs were verified by qRT-PCR assay. Results Our observations suggested that anp-1 mutation induced small body size in the L4/young adult stage of C. elegans, however, there was no difference between N2 and RB804 in adult stage. Moreover, deletion of anp-1 resulted in shortening lifespan and laying fewer eggs. DEGs (184 genes) were observed between N2 groups and RB804 groups by transcriptome sequencing. According to GO annotations and KEGG enrichment analysis, these DEGs play vital roles in development regulation in C. elegans. These data demonstrate ANP-1 participates in development and aging of C. elegans and will considerably contribute to the existing knowledge of aminopeptidase function in C. elegans. |
| first_indexed | 2024-03-09T06:31:37Z |
| format | Article |
| id | doaj.art-f7e0f0037bbe41c582d54f43670b1a27 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:31:37Z |
| publishDate | 2019-11-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-f7e0f0037bbe41c582d54f43670b1a272023-12-03T11:05:02ZengPeerJ Inc.PeerJ2167-83592019-11-017e794410.7717/peerj.7944Characterization of aminopeptidase encoding gene anp-1 and its association with development in Caenorhabditis elegansShanchun Su0Baoliang Pan1Yanxin Hu2Ming Wang3Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, Beijing, ChinaKey Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, Beijing, ChinaKey Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, Beijing, ChinaKey Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, Beijing, ChinaBackground Aminopeptidases play important roles in various biological processes in nematodes including growth, development and reproduction. Although the aminopeptidases have been shown to regulate reproduction in Caenorhabditis elegans (C. elegans), the role of aminopeptidases in development and aging has not been reported. This study focused on the function of aminopeptidase AlaNyl aminopeptidase 1 (ANP-1) on development in C. elegans. Methods In the present study, we reported the identification of ANP-1 in C. elegans along with sequence analysis and its functional expression and characterization. The phenotype changes were observed when anp-1 mutated. Then, differential expression genes (DEGs) between wild type strain (N2) and anp-1 deletion strain (RB804) were identified using transcriptome sequencing method. Finally, DEGs were verified by qRT-PCR assay. Results Our observations suggested that anp-1 mutation induced small body size in the L4/young adult stage of C. elegans, however, there was no difference between N2 and RB804 in adult stage. Moreover, deletion of anp-1 resulted in shortening lifespan and laying fewer eggs. DEGs (184 genes) were observed between N2 groups and RB804 groups by transcriptome sequencing. According to GO annotations and KEGG enrichment analysis, these DEGs play vital roles in development regulation in C. elegans. These data demonstrate ANP-1 participates in development and aging of C. elegans and will considerably contribute to the existing knowledge of aminopeptidase function in C. elegans.https://peerj.com/articles/7944.pdfCaenorhabditis elegansAminopeptidasesLifespanBody size |
| spellingShingle | Shanchun Su Baoliang Pan Yanxin Hu Ming Wang Characterization of aminopeptidase encoding gene anp-1 and its association with development in Caenorhabditis elegans PeerJ Caenorhabditis elegans Aminopeptidases Lifespan Body size |
| title | Characterization of aminopeptidase encoding gene anp-1 and its association with development in Caenorhabditis elegans |
| title_full | Characterization of aminopeptidase encoding gene anp-1 and its association with development in Caenorhabditis elegans |
| title_fullStr | Characterization of aminopeptidase encoding gene anp-1 and its association with development in Caenorhabditis elegans |
| title_full_unstemmed | Characterization of aminopeptidase encoding gene anp-1 and its association with development in Caenorhabditis elegans |
| title_short | Characterization of aminopeptidase encoding gene anp-1 and its association with development in Caenorhabditis elegans |
| title_sort | characterization of aminopeptidase encoding gene anp 1 and its association with development in caenorhabditis elegans |
| topic | Caenorhabditis elegans Aminopeptidases Lifespan Body size |
| url | https://peerj.com/articles/7944.pdf |
| work_keys_str_mv | AT shanchunsu characterizationofaminopeptidaseencodinggeneanp1anditsassociationwithdevelopmentincaenorhabditiselegans AT baoliangpan characterizationofaminopeptidaseencodinggeneanp1anditsassociationwithdevelopmentincaenorhabditiselegans AT yanxinhu characterizationofaminopeptidaseencodinggeneanp1anditsassociationwithdevelopmentincaenorhabditiselegans AT mingwang characterizationofaminopeptidaseencodinggeneanp1anditsassociationwithdevelopmentincaenorhabditiselegans |