lncRNA TM4SF1-AS1 Activates the PI3K/AKT Signaling Pathway and Promotes the Migration and Invasion of Lung Cancer Cells
Fachen Zhou,1,2,* Jin Wang,2,* Xinming Chi,3 Xin Zhou,3 Zhou Wang1 1Department of Thoracic Surgery, Shandong Provincial Hospital Affiliated with Shandong University, Jinan, Shandong, People’s Republic of China; 2Department of Thoracic Surgery, The First Affiliated Hospital of Dalian Medica...
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Dove Medical Press
2020-07-01
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| Series: | Cancer Management and Research |
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| Online Access: | https://www.dovepress.com/lncrna-tm4sf1-as1-activates-the-pi3kakt-signaling-pathway-and-promotes-peer-reviewed-article-CMAR |
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| author | Zhou F Wang J Chi X Zhou X Wang Z |
| author_facet | Zhou F Wang J Chi X Zhou X Wang Z |
| author_sort | Zhou F |
| collection | DOAJ |
| description | Fachen Zhou,1,2,* Jin Wang,2,* Xinming Chi,3 Xin Zhou,3 Zhou Wang1 1Department of Thoracic Surgery, Shandong Provincial Hospital Affiliated with Shandong University, Jinan, Shandong, People’s Republic of China; 2Department of Thoracic Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, People’s Republic of China; 3Department of Histology and Embryology, Dalian Medical University, Dalian, Liaoning, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhou WangDepartment of Thoracic Surgery, Shandong Provincial Hospital Affiliated to Shandong University, No. 324 Jingwuweiqi Road, Jinan, Shandong Province 250021, People’s Republic of ChinaTel +86 53 168777893Email wangzhoushandong@163.comXin ZhouDepartment of Histology and Embryology, Dalian Medical University, No. 9 West Part of Lvshun South Road, Dalian, Liaoning Province, People’s Republic of ChinaTel +86 41 186110314Email 18642818055@163.comPurpose: Metastasis is a crucial cause of the high mortality in patients with lung cancer. Long non-coding RNAs (lncRNAs) are emerging as important players in the development and progression of human cancers. Here, we aimed to identify metastasis-associated lncRNA and to study its roles in the migration and invasion of lung cancer cells.Materials and Methods: We screened differentially expressed lncRNAs between high- and low-metastatic lung cancer cell lines by using microarray and identified the target lncRNA TM4SF1-AS1. The effect of the TM4SF1-AS1 on the invasion and migration was evaluated through the wound healing experiment and transwell assay. The expression of related genes was assessed by RNA sequence and Western blotting.Results: TM4SF1-AS1 was highly expressed in high metastatic lung cancer cell line, and it was also significantly up-regulated in lymph node metastatic lung cancer and was associated with lymph node metastasis. Overexpression of TM4SF1-AS1 promoted the migration and invasion of lung cancer cells. Overexpression of TM4SF1-AS1 decreased the expression of E-Cadherin and increased the expression of Vimentin, Snail and Twist, while knockdown of TM4SF1-AS1 exhibited the opposite trend. Furthermore, RNA sequence analysis revealed that some signaling pathways, including PI3K/AKT signaling pathway, were enriched upon TM4SF1-AS1 overexpression. Western blotting further confirmed that the PI3K/AKT signaling pathway was activated by TM4SF1-AS1.Conclusion: This study illustrates that TM4SF1-AS1 promotes the migration and invasion of lung cancer cells by activating the PI3K/AKT signaling pathway. TM4SF1-AS1 might be a novel target of molecular treatment for lung cancer.Keywords: lung cancer, long non-coding RNA, TM4SF1-AS1, metastasis, PI3K/AKT signaling pathway |
| first_indexed | 2024-12-11T12:12:25Z |
| format | Article |
| id | doaj.art-f7e26a9ceb4c41519094c21884c7a3cd |
| institution | Directory Open Access Journal |
| issn | 1179-1322 |
| language | English |
| last_indexed | 2024-12-11T12:12:25Z |
| publishDate | 2020-07-01 |
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| series | Cancer Management and Research |
| spelling | doaj.art-f7e26a9ceb4c41519094c21884c7a3cd2022-12-22T01:07:46ZengDove Medical PressCancer Management and Research1179-13222020-07-01Volume 125527553655156lncRNA TM4SF1-AS1 Activates the PI3K/AKT Signaling Pathway and Promotes the Migration and Invasion of Lung Cancer CellsZhou FWang JChi XZhou XWang ZFachen Zhou,1,2,* Jin Wang,2,* Xinming Chi,3 Xin Zhou,3 Zhou Wang1 1Department of Thoracic Surgery, Shandong Provincial Hospital Affiliated with Shandong University, Jinan, Shandong, People’s Republic of China; 2Department of Thoracic Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, People’s Republic of China; 3Department of Histology and Embryology, Dalian Medical University, Dalian, Liaoning, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhou WangDepartment of Thoracic Surgery, Shandong Provincial Hospital Affiliated to Shandong University, No. 324 Jingwuweiqi Road, Jinan, Shandong Province 250021, People’s Republic of ChinaTel +86 53 168777893Email wangzhoushandong@163.comXin ZhouDepartment of Histology and Embryology, Dalian Medical University, No. 9 West Part of Lvshun South Road, Dalian, Liaoning Province, People’s Republic of ChinaTel +86 41 186110314Email 18642818055@163.comPurpose: Metastasis is a crucial cause of the high mortality in patients with lung cancer. Long non-coding RNAs (lncRNAs) are emerging as important players in the development and progression of human cancers. Here, we aimed to identify metastasis-associated lncRNA and to study its roles in the migration and invasion of lung cancer cells.Materials and Methods: We screened differentially expressed lncRNAs between high- and low-metastatic lung cancer cell lines by using microarray and identified the target lncRNA TM4SF1-AS1. The effect of the TM4SF1-AS1 on the invasion and migration was evaluated through the wound healing experiment and transwell assay. The expression of related genes was assessed by RNA sequence and Western blotting.Results: TM4SF1-AS1 was highly expressed in high metastatic lung cancer cell line, and it was also significantly up-regulated in lymph node metastatic lung cancer and was associated with lymph node metastasis. Overexpression of TM4SF1-AS1 promoted the migration and invasion of lung cancer cells. Overexpression of TM4SF1-AS1 decreased the expression of E-Cadherin and increased the expression of Vimentin, Snail and Twist, while knockdown of TM4SF1-AS1 exhibited the opposite trend. Furthermore, RNA sequence analysis revealed that some signaling pathways, including PI3K/AKT signaling pathway, were enriched upon TM4SF1-AS1 overexpression. Western blotting further confirmed that the PI3K/AKT signaling pathway was activated by TM4SF1-AS1.Conclusion: This study illustrates that TM4SF1-AS1 promotes the migration and invasion of lung cancer cells by activating the PI3K/AKT signaling pathway. TM4SF1-AS1 might be a novel target of molecular treatment for lung cancer.Keywords: lung cancer, long non-coding RNA, TM4SF1-AS1, metastasis, PI3K/AKT signaling pathwayhttps://www.dovepress.com/lncrna-tm4sf1-as1-activates-the-pi3kakt-signaling-pathway-and-promotes-peer-reviewed-article-CMARlung cancerlong non-coding rnatm4sf1-as1metastasispi3k/akt signaling pathway |
| spellingShingle | Zhou F Wang J Chi X Zhou X Wang Z lncRNA TM4SF1-AS1 Activates the PI3K/AKT Signaling Pathway and Promotes the Migration and Invasion of Lung Cancer Cells Cancer Management and Research lung cancer long non-coding rna tm4sf1-as1 metastasis pi3k/akt signaling pathway |
| title | lncRNA TM4SF1-AS1 Activates the PI3K/AKT Signaling Pathway and Promotes the Migration and Invasion of Lung Cancer Cells |
| title_full | lncRNA TM4SF1-AS1 Activates the PI3K/AKT Signaling Pathway and Promotes the Migration and Invasion of Lung Cancer Cells |
| title_fullStr | lncRNA TM4SF1-AS1 Activates the PI3K/AKT Signaling Pathway and Promotes the Migration and Invasion of Lung Cancer Cells |
| title_full_unstemmed | lncRNA TM4SF1-AS1 Activates the PI3K/AKT Signaling Pathway and Promotes the Migration and Invasion of Lung Cancer Cells |
| title_short | lncRNA TM4SF1-AS1 Activates the PI3K/AKT Signaling Pathway and Promotes the Migration and Invasion of Lung Cancer Cells |
| title_sort | lncrna tm4sf1 as1 activates the pi3k akt signaling pathway and promotes the migration and invasion of lung cancer cells |
| topic | lung cancer long non-coding rna tm4sf1-as1 metastasis pi3k/akt signaling pathway |
| url | https://www.dovepress.com/lncrna-tm4sf1-as1-activates-the-pi3kakt-signaling-pathway-and-promotes-peer-reviewed-article-CMAR |
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