Differentially expressed genes in visceral or subcutaneous adipose tissue of obese men and women
There is growing evidence that the distribution of adipose tissue in the body is of importance in the development of metabolic complications of obesity, such as diabetes, hypertension, and hyperlipidemia. The aim of this study was to identify differentially expressed genes in subcutaneous and omenta...
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| Language: | English |
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Elsevier
2004-01-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520319313 |
| _version_ | 1823937609214197760 |
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| author | Kristina Linder Peter Arner Amilcar Flores-Morales Petra Tollet-Egnell Gunnar Norstedt |
| author_facet | Kristina Linder Peter Arner Amilcar Flores-Morales Petra Tollet-Egnell Gunnar Norstedt |
| author_sort | Kristina Linder |
| collection | DOAJ |
| description | There is growing evidence that the distribution of adipose tissue in the body is of importance in the development of metabolic complications of obesity, such as diabetes, hypertension, and hyperlipidemia. The aim of this study was to identify differentially expressed genes in subcutaneous and omental human adipose tissue in obese men, using a subtractive hybridization strategy. From the obtained set of differentially expressed transcripts, we also aimed to identify genes that have a sex-specific pattern of expression in omental or subcutaneous adipose tissue. Representational difference analysis (RDA) was performed on cDNA from subcutaneous and omental fat tissue from a man with extreme abdominal obesity. Forty-four putatively differentially expressed genes were identified. The obtained RDA products were spotted onto glass slides to screen for differential expression in other obese patients by using a microarray hybridization procedure. Five genes were confirmed to be differentially expressed in subcutaneous or omental adipose tissue from male or female obese patients. One gene was detected only in males and was found to be upregulated in subcutaneous tissue.The findings extend previous knowledge that different fat depots have differential gene expression and indicate that sex differences exist in adipose gene expression patterns. Linder, K., P. Arner, A. Flores-Morales, P. Tollet-Egnell, and G. Norstedt. Differentially expressed genes in visceral or subcutaneous adipose tissue of obese men and women. J. Lipid Res. 2004. 45: 148–154. |
| first_indexed | 2024-12-17T02:04:07Z |
| format | Article |
| id | doaj.art-f7e273aa44d44a3ab372b3c57b73a9b6 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T02:04:07Z |
| publishDate | 2004-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-f7e273aa44d44a3ab372b3c57b73a9b62022-12-21T22:07:45ZengElsevierJournal of Lipid Research0022-22752004-01-01451148154Differentially expressed genes in visceral or subcutaneous adipose tissue of obese men and womenKristina Linder0Peter Arner1Amilcar Flores-Morales2Petra Tollet-Egnell3Gunnar Norstedt4Department of Molecular Medicine, Karolinska Hospital, Stockholm, Sweden; Institution for Medicine, Huddinge University Hospital, Karolinska Institutet, Stockholm, SwedenDepartment of Molecular Medicine, Karolinska Hospital, Stockholm, Sweden; Institution for Medicine, Huddinge University Hospital, Karolinska Institutet, Stockholm, SwedenDepartment of Molecular Medicine, Karolinska Hospital, Stockholm, Sweden; Institution for Medicine, Huddinge University Hospital, Karolinska Institutet, Stockholm, SwedenDepartment of Molecular Medicine, Karolinska Hospital, Stockholm, Sweden; Institution for Medicine, Huddinge University Hospital, Karolinska Institutet, Stockholm, SwedenDepartment of Molecular Medicine, Karolinska Hospital, Stockholm, Sweden; Institution for Medicine, Huddinge University Hospital, Karolinska Institutet, Stockholm, SwedenThere is growing evidence that the distribution of adipose tissue in the body is of importance in the development of metabolic complications of obesity, such as diabetes, hypertension, and hyperlipidemia. The aim of this study was to identify differentially expressed genes in subcutaneous and omental human adipose tissue in obese men, using a subtractive hybridization strategy. From the obtained set of differentially expressed transcripts, we also aimed to identify genes that have a sex-specific pattern of expression in omental or subcutaneous adipose tissue. Representational difference analysis (RDA) was performed on cDNA from subcutaneous and omental fat tissue from a man with extreme abdominal obesity. Forty-four putatively differentially expressed genes were identified. The obtained RDA products were spotted onto glass slides to screen for differential expression in other obese patients by using a microarray hybridization procedure. Five genes were confirmed to be differentially expressed in subcutaneous or omental adipose tissue from male or female obese patients. One gene was detected only in males and was found to be upregulated in subcutaneous tissue.The findings extend previous knowledge that different fat depots have differential gene expression and indicate that sex differences exist in adipose gene expression patterns. Linder, K., P. Arner, A. Flores-Morales, P. Tollet-Egnell, and G. Norstedt. Differentially expressed genes in visceral or subcutaneous adipose tissue of obese men and women. J. Lipid Res. 2004. 45: 148–154.http://www.sciencedirect.com/science/article/pii/S0022227520319313representational difference analysisdifferential gene expressionDNA microarray analysisadipsinrasphospholipid transfer |
| spellingShingle | Kristina Linder Peter Arner Amilcar Flores-Morales Petra Tollet-Egnell Gunnar Norstedt Differentially expressed genes in visceral or subcutaneous adipose tissue of obese men and women Journal of Lipid Research representational difference analysis differential gene expression DNA microarray analysis adipsin ras phospholipid transfer |
| title | Differentially expressed genes in visceral or subcutaneous adipose tissue of obese men and women |
| title_full | Differentially expressed genes in visceral or subcutaneous adipose tissue of obese men and women |
| title_fullStr | Differentially expressed genes in visceral or subcutaneous adipose tissue of obese men and women |
| title_full_unstemmed | Differentially expressed genes in visceral or subcutaneous adipose tissue of obese men and women |
| title_short | Differentially expressed genes in visceral or subcutaneous adipose tissue of obese men and women |
| title_sort | differentially expressed genes in visceral or subcutaneous adipose tissue of obese men and women |
| topic | representational difference analysis differential gene expression DNA microarray analysis adipsin ras phospholipid transfer |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520319313 |
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