Ultrasound-Assisted Extraction of Flavonoids from <i>Potentilla fruticosa</i> L. Using Natural Deep Eutectic Solvents
A series of natural deep eutectic solvents (NADESs) were prepared with choline chloride, betaine, and a variety of natural organic acids in order to find new environmentally-friendly green solvents to replace the traditional solvents. The NADESs were employed to extract flavonoids from <i>Pote...
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MDPI AG
2022-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/18/5794 |
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| author | He Xue Jinping Li Guiyao Wang Wenming Zuo Yang Zeng Likuan Liu |
| author_facet | He Xue Jinping Li Guiyao Wang Wenming Zuo Yang Zeng Likuan Liu |
| author_sort | He Xue |
| collection | DOAJ |
| description | A series of natural deep eutectic solvents (NADESs) were prepared with choline chloride, betaine, and a variety of natural organic acids in order to find new environmentally-friendly green solvents to replace the traditional solvents. The NADESs were employed to extract flavonoids from <i>Potentilla fruticosa</i> L. (<i>PFL</i>) with the help of ultrasound. The eutectic solvent diluted with an appropriate amount of water improved the extraction ability of flavonoids due to the decrease of solution viscosity. The microstructure of the raw sample and the samples subjected to ultrasonic bath in different solutions were observed using scanning electron microscope (SEM) to determine the role of the NADESs in the extraction process. The DPPH method and glucose consumption method were used to study the antioxidant and hypoglycemic ability of flavonoid compounds in <i>PFL</i>. Single factor method and response surface methodology (RSM) were designed to analyze the effects of three extraction parameters, including solvent/solid ratio, ultrasonic power, and extraction time, on the extraction yield, antioxidant capacity, and hypoglycemic capacity, and the corresponding second-order polynomial prediction models were established. The optimal extraction conditions for the maximum extraction yield, antioxidant capacity, and hypoglycemic capacity were predicted by RSM, and the reliability of RSM simulation results was verified by a one-off experiment. |
| first_indexed | 2024-03-09T23:03:29Z |
| format | Article |
| id | doaj.art-f7e4e74ade8540a696dc6b9cf389e4f1 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T23:03:29Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj.art-f7e4e74ade8540a696dc6b9cf389e4f12023-11-23T17:59:00ZengMDPI AGMolecules1420-30492022-09-012718579410.3390/molecules27185794Ultrasound-Assisted Extraction of Flavonoids from <i>Potentilla fruticosa</i> L. Using Natural Deep Eutectic SolventsHe Xue0Jinping Li1Guiyao Wang2Wenming Zuo3Yang Zeng4Likuan Liu5College of Life Science, Qing Hai Normal University, Xining 810008, ChinaCollege of Life Science, Qing Hai Normal University, Xining 810008, ChinaCollege of Life Science, Qing Hai Normal University, Xining 810008, ChinaCollege of Life Science, Qing Hai Normal University, Xining 810008, ChinaCollege of Life Science, Qing Hai Normal University, Xining 810008, ChinaCollege of Life Science, Qing Hai Normal University, Xining 810008, ChinaA series of natural deep eutectic solvents (NADESs) were prepared with choline chloride, betaine, and a variety of natural organic acids in order to find new environmentally-friendly green solvents to replace the traditional solvents. The NADESs were employed to extract flavonoids from <i>Potentilla fruticosa</i> L. (<i>PFL</i>) with the help of ultrasound. The eutectic solvent diluted with an appropriate amount of water improved the extraction ability of flavonoids due to the decrease of solution viscosity. The microstructure of the raw sample and the samples subjected to ultrasonic bath in different solutions were observed using scanning electron microscope (SEM) to determine the role of the NADESs in the extraction process. The DPPH method and glucose consumption method were used to study the antioxidant and hypoglycemic ability of flavonoid compounds in <i>PFL</i>. Single factor method and response surface methodology (RSM) were designed to analyze the effects of three extraction parameters, including solvent/solid ratio, ultrasonic power, and extraction time, on the extraction yield, antioxidant capacity, and hypoglycemic capacity, and the corresponding second-order polynomial prediction models were established. The optimal extraction conditions for the maximum extraction yield, antioxidant capacity, and hypoglycemic capacity were predicted by RSM, and the reliability of RSM simulation results was verified by a one-off experiment.https://www.mdpi.com/1420-3049/27/18/5794natural deep eutectic solventsultrasound-assisted extraction<i>Potentilla fruticosa</i> L.response surface methodology |
| spellingShingle | He Xue Jinping Li Guiyao Wang Wenming Zuo Yang Zeng Likuan Liu Ultrasound-Assisted Extraction of Flavonoids from <i>Potentilla fruticosa</i> L. Using Natural Deep Eutectic Solvents Molecules natural deep eutectic solvents ultrasound-assisted extraction <i>Potentilla fruticosa</i> L. response surface methodology |
| title | Ultrasound-Assisted Extraction of Flavonoids from <i>Potentilla fruticosa</i> L. Using Natural Deep Eutectic Solvents |
| title_full | Ultrasound-Assisted Extraction of Flavonoids from <i>Potentilla fruticosa</i> L. Using Natural Deep Eutectic Solvents |
| title_fullStr | Ultrasound-Assisted Extraction of Flavonoids from <i>Potentilla fruticosa</i> L. Using Natural Deep Eutectic Solvents |
| title_full_unstemmed | Ultrasound-Assisted Extraction of Flavonoids from <i>Potentilla fruticosa</i> L. Using Natural Deep Eutectic Solvents |
| title_short | Ultrasound-Assisted Extraction of Flavonoids from <i>Potentilla fruticosa</i> L. Using Natural Deep Eutectic Solvents |
| title_sort | ultrasound assisted extraction of flavonoids from i potentilla fruticosa i l using natural deep eutectic solvents |
| topic | natural deep eutectic solvents ultrasound-assisted extraction <i>Potentilla fruticosa</i> L. response surface methodology |
| url | https://www.mdpi.com/1420-3049/27/18/5794 |
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