Pollen Competition and Paternal Contribution during Artificially Controlled Pollination of Black Locust (<i>Robinia pseudoacacia</i> L.) without Castration
(1) Background: Considering the serious damage caused by castration and the extremely high outcrossing rate in nature, we hypothesized that artificial controlled pollination of black locust without castration could be conducted for hybridization breeding. (2) Methods: The study conducted controlled...
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2021-10-01
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| Online Access: | https://www.mdpi.com/1999-4907/12/10/1416 |
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| author | Yuhan Sun Ruiyang Hu Li Dong Xiuyu Li Zijie Zhang Qi Guo Sen Cao Jiankang Li Peiyao Han Chao Han Saleem Uddin Cui Long Yingming Fan Yun Li |
| author_facet | Yuhan Sun Ruiyang Hu Li Dong Xiuyu Li Zijie Zhang Qi Guo Sen Cao Jiankang Li Peiyao Han Chao Han Saleem Uddin Cui Long Yingming Fan Yun Li |
| author_sort | Yuhan Sun |
| collection | DOAJ |
| description | (1) Background: Considering the serious damage caused by castration and the extremely high outcrossing rate in nature, we hypothesized that artificial controlled pollination of black locust without castration could be conducted for hybridization breeding. (2) Methods: The study conducted controlled pollination on 20 mating combinations of black locust without castration using a single or mixed male parent. Offspring of different developmental stages and the leaves of parents were collected to extract DNA and perform paternity analysis using SSR molecular markers. (3) Results: The contribution rate of each male parent differed according to developmental stage after pollination using different pollens mixed in equal proportions. There were significant correlations between the genetic similarity between each male parent and female parent and contribution rate of each male parent at three different developmental stages after pollination. (4) Conclusions: The composition of offspring pollen donors showed no bias toward selfing or outcrossing when artificially pollinated without castration. Hybrid breeding of black locust by artificially controlled pollination without castration may not be feasible, given that our manual method resulted in a large number of abortive and abnormal offspring. Introduction of honeybees in a limited space to conduct controlled pollination of black locust for hybrid breeding may be feasible. |
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| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-10T06:33:16Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
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| series | Forests |
| spelling | doaj.art-f7ebf2227a054f1288f83d4738f81cbb2023-11-22T18:19:21ZengMDPI AGForests1999-49072021-10-011210141610.3390/f12101416Pollen Competition and Paternal Contribution during Artificially Controlled Pollination of Black Locust (<i>Robinia pseudoacacia</i> L.) without CastrationYuhan Sun0Ruiyang Hu1Li Dong2Xiuyu Li3Zijie Zhang4Qi Guo5Sen Cao6Jiankang Li7Peiyao Han8Chao Han9Saleem Uddin10Cui Long11Yingming Fan12Yun Li13Engineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaExperimental Center of Forestry in North China, Chinese Academy of Forestry, National Permanent Scientific Research Base for Warm Temperate Zone Forestry of Jiulong Mountain in Beijing, Beijing 102300, ChinaEngineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaEngineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaEngineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaEngineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaEngineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaEngineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaEngineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaEngineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaEngineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaEngineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaEngineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaEngineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China(1) Background: Considering the serious damage caused by castration and the extremely high outcrossing rate in nature, we hypothesized that artificial controlled pollination of black locust without castration could be conducted for hybridization breeding. (2) Methods: The study conducted controlled pollination on 20 mating combinations of black locust without castration using a single or mixed male parent. Offspring of different developmental stages and the leaves of parents were collected to extract DNA and perform paternity analysis using SSR molecular markers. (3) Results: The contribution rate of each male parent differed according to developmental stage after pollination using different pollens mixed in equal proportions. There were significant correlations between the genetic similarity between each male parent and female parent and contribution rate of each male parent at three different developmental stages after pollination. (4) Conclusions: The composition of offspring pollen donors showed no bias toward selfing or outcrossing when artificially pollinated without castration. Hybrid breeding of black locust by artificially controlled pollination without castration may not be feasible, given that our manual method resulted in a large number of abortive and abnormal offspring. Introduction of honeybees in a limited space to conduct controlled pollination of black locust for hybrid breeding may be feasible.https://www.mdpi.com/1999-4907/12/10/1416<i>Robinia pseudoacacia</i> L.hybridization breedingartificial controlled pollinationwithout castrationpollen competitionoutcrossing rate |
| spellingShingle | Yuhan Sun Ruiyang Hu Li Dong Xiuyu Li Zijie Zhang Qi Guo Sen Cao Jiankang Li Peiyao Han Chao Han Saleem Uddin Cui Long Yingming Fan Yun Li Pollen Competition and Paternal Contribution during Artificially Controlled Pollination of Black Locust (<i>Robinia pseudoacacia</i> L.) without Castration Forests <i>Robinia pseudoacacia</i> L. hybridization breeding artificial controlled pollination without castration pollen competition outcrossing rate |
| title | Pollen Competition and Paternal Contribution during Artificially Controlled Pollination of Black Locust (<i>Robinia pseudoacacia</i> L.) without Castration |
| title_full | Pollen Competition and Paternal Contribution during Artificially Controlled Pollination of Black Locust (<i>Robinia pseudoacacia</i> L.) without Castration |
| title_fullStr | Pollen Competition and Paternal Contribution during Artificially Controlled Pollination of Black Locust (<i>Robinia pseudoacacia</i> L.) without Castration |
| title_full_unstemmed | Pollen Competition and Paternal Contribution during Artificially Controlled Pollination of Black Locust (<i>Robinia pseudoacacia</i> L.) without Castration |
| title_short | Pollen Competition and Paternal Contribution during Artificially Controlled Pollination of Black Locust (<i>Robinia pseudoacacia</i> L.) without Castration |
| title_sort | pollen competition and paternal contribution during artificially controlled pollination of black locust i robinia pseudoacacia i l without castration |
| topic | <i>Robinia pseudoacacia</i> L. hybridization breeding artificial controlled pollination without castration pollen competition outcrossing rate |
| url | https://www.mdpi.com/1999-4907/12/10/1416 |
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