Synergistic inhibitory effect of α-humulene and sclareol on human pancreatic cancer cells
Both α-Humulene (HUM) and sclareol (SCL) are naturally occurred compounds and have been shown to exert anti-inflammatory and anticancer activities. The effects of these two compounds alone or in combination on pancreatic cancer cells have not been reported. In the present study, we investigated the...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-02-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464622000287 |
| _version_ | 1819291681449574400 |
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| author | Yu Kang Xiao Wang Xingchuan Wei Dongli Li Lishe Gan Jingwei Jin Rihui Wu Panpan Wu Zhaojun Sheng Kun Zhang Susan Goodin Xuetao Xu Xi Zheng |
| author_facet | Yu Kang Xiao Wang Xingchuan Wei Dongli Li Lishe Gan Jingwei Jin Rihui Wu Panpan Wu Zhaojun Sheng Kun Zhang Susan Goodin Xuetao Xu Xi Zheng |
| author_sort | Yu Kang |
| collection | DOAJ |
| description | Both α-Humulene (HUM) and sclareol (SCL) are naturally occurred compounds and have been shown to exert anti-inflammatory and anticancer activities. The effects of these two compounds alone or in combination on pancreatic cancer cells have not been reported. In the present study, we investigated the effects of HUM and SCL on human pancreatic cancer cells cultured in vitro and grown as xenograft tumors in vivo. In the in vitro studies, we found that HUM and SCL in combination synergistically inhibited the growth of pancreatic cancer cells. The combination strongly stimulated apoptosis, and suppressed migration, invasion and sphere formation in pancreatic cells. Studies on the mechanism of action showed that the combination strongly decreased the levels of NF-κB, phosphor Akt (pAkt) and Bcl-2, and increased the level of Bax. Knockdown of NF-κB and Akt by small interfering RNA (siRNA) abrogated the inhibitory effect of HUM and SCL on pancreatic cancer cells. The in vivo study showed that HUM and SCL in combination suppressed the growth of Panc-1 xenograft tumors in immunodeficient mice. Results of the present study indicate that HUM and SCL in combination may represent an effective approach for inhibiting the growth of pancreatic cancer. |
| first_indexed | 2024-12-24T03:42:30Z |
| format | Article |
| id | doaj.art-f7ef0a582a154f18954b8f73b8a23a4b |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-24T03:42:30Z |
| publishDate | 2022-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-f7ef0a582a154f18954b8f73b8a23a4b2022-12-21T17:16:52ZengElsevierJournal of Functional Foods1756-46462022-02-0189104958Synergistic inhibitory effect of α-humulene and sclareol on human pancreatic cancer cellsYu Kang0Xiao Wang1Xingchuan Wei2Dongli Li3Lishe Gan4Jingwei Jin5Rihui Wu6Panpan Wu7Zhaojun Sheng8Kun Zhang9Susan Goodin10Xuetao Xu11Xi Zheng12School of Biotechnology and Health Sciences, Wuyi University, Jiangmen City, Guangdong Province 529020, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen City, Guangdong Province 529020, ChinaDepartment of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou 510006, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen City, Guangdong Province 529020, China; International Healthcare Innovation Institute (Jiangmen), Jiangmen City, Guangdong Province 529020, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen City, Guangdong Province 529020, China; International Healthcare Innovation Institute (Jiangmen), Jiangmen City, Guangdong Province 529020, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen City, Guangdong Province 529020, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen City, Guangdong Province 529020, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen City, Guangdong Province 529020, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen City, Guangdong Province 529020, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen City, Guangdong Province 529020, ChinaRutgers Cancer Institute of New Jersey, New Brunswick, NJ 08903, USASchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen City, Guangdong Province 529020, China; Corresponding authors.Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA; Corresponding authors.Both α-Humulene (HUM) and sclareol (SCL) are naturally occurred compounds and have been shown to exert anti-inflammatory and anticancer activities. The effects of these two compounds alone or in combination on pancreatic cancer cells have not been reported. In the present study, we investigated the effects of HUM and SCL on human pancreatic cancer cells cultured in vitro and grown as xenograft tumors in vivo. In the in vitro studies, we found that HUM and SCL in combination synergistically inhibited the growth of pancreatic cancer cells. The combination strongly stimulated apoptosis, and suppressed migration, invasion and sphere formation in pancreatic cells. Studies on the mechanism of action showed that the combination strongly decreased the levels of NF-κB, phosphor Akt (pAkt) and Bcl-2, and increased the level of Bax. Knockdown of NF-κB and Akt by small interfering RNA (siRNA) abrogated the inhibitory effect of HUM and SCL on pancreatic cancer cells. The in vivo study showed that HUM and SCL in combination suppressed the growth of Panc-1 xenograft tumors in immunodeficient mice. Results of the present study indicate that HUM and SCL in combination may represent an effective approach for inhibiting the growth of pancreatic cancer.http://www.sciencedirect.com/science/article/pii/S1756464622000287α-HumuleneSclareolPancreatic cancerApoptosisMigrationInvasion |
| spellingShingle | Yu Kang Xiao Wang Xingchuan Wei Dongli Li Lishe Gan Jingwei Jin Rihui Wu Panpan Wu Zhaojun Sheng Kun Zhang Susan Goodin Xuetao Xu Xi Zheng Synergistic inhibitory effect of α-humulene and sclareol on human pancreatic cancer cells Journal of Functional Foods α-Humulene Sclareol Pancreatic cancer Apoptosis Migration Invasion |
| title | Synergistic inhibitory effect of α-humulene and sclareol on human pancreatic cancer cells |
| title_full | Synergistic inhibitory effect of α-humulene and sclareol on human pancreatic cancer cells |
| title_fullStr | Synergistic inhibitory effect of α-humulene and sclareol on human pancreatic cancer cells |
| title_full_unstemmed | Synergistic inhibitory effect of α-humulene and sclareol on human pancreatic cancer cells |
| title_short | Synergistic inhibitory effect of α-humulene and sclareol on human pancreatic cancer cells |
| title_sort | synergistic inhibitory effect of α humulene and sclareol on human pancreatic cancer cells |
| topic | α-Humulene Sclareol Pancreatic cancer Apoptosis Migration Invasion |
| url | http://www.sciencedirect.com/science/article/pii/S1756464622000287 |
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