Targeted metabolomics reveals serum changes of amino acids in mild to moderate ischemic stroke and stroke mimics
BackgroundThe pathophysiological processes linked to an acute ischemic stroke (IS) can be reflected in the circulating metabolome. Amino acids (AAs) have been demonstrated to be one of the most significant metabolites that can undergo significant alteration after a stroke.MethodsWe sought to identif...
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Neurology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fneur.2023.1153193/full |
| _version_ | 1797847196313321472 |
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| author | Shuxin Tao Xinxing Xiao Xin Li Fan Na Guo Na Shuang Wang Pin Zhang Fang Hao Peiran Zhao Dong Guo Xuewu Liu Dawei Yang |
| author_facet | Shuxin Tao Xinxing Xiao Xin Li Fan Na Guo Na Shuang Wang Pin Zhang Fang Hao Peiran Zhao Dong Guo Xuewu Liu Dawei Yang |
| author_sort | Shuxin Tao |
| collection | DOAJ |
| description | BackgroundThe pathophysiological processes linked to an acute ischemic stroke (IS) can be reflected in the circulating metabolome. Amino acids (AAs) have been demonstrated to be one of the most significant metabolites that can undergo significant alteration after a stroke.MethodsWe sought to identify the potential biomarkers for the early detection of IS using an extensive targeted technique for reliable quantification of 27 different AAs based on ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). A cohort with 216 participants was enrolled, including 70 mild to moderate ischemic stroke patients (National Institutes of Health Stroke Scale < 15, MB group), 76 stroke mimics (MM group) and 70 healthy controls (NC group).ResultsIt was found that upon comparing MB and MM to control patients, AAs shifts were detected via partial least squares discrimination analysis (PLS-DA) and pathway analysis. Interestingly, MB and MM exhibited similar AAs pattern. Moreover, ornithine, asparagine, valine, citrulline, and cysteine were identified for inclusion in a biomarker panel for early-stage stroke detection based upon an AUC of 0.968 (95% CI 0.924–0.998). Levels of ornithine were positively associated with infract volume, 3 months mRS score, and National Institutes of Health Stroke Scale (NIHSS) score in MB. In addition, a metabolites biomarker panel, including ornithine, taurine, phenylalanine, citrulline, cysteine, yielded an AUC of 0.99 (95% CI 0.966–1) which can be employed to effectively discriminate MM patients from control.ConclusionOverall, alternations in serum AAs are characteristic metabolic features of MB and MM. AAs could serve as promising biomarkers for the early diagnosis of MB patients since mild to moderate IS patients were enrolled in the study. The metabolism of AAs can be considered as a key indicator for both the prevention and treatment of IS. |
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| institution | Directory Open Access Journal |
| issn | 1664-2295 |
| language | English |
| last_indexed | 2024-04-09T18:07:23Z |
| publishDate | 2023-04-01 |
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| series | Frontiers in Neurology |
| spelling | doaj.art-f7f1a76bfe654e93be97087a4374a5a12023-04-14T05:40:13ZengFrontiers Media S.A.Frontiers in Neurology1664-22952023-04-011410.3389/fneur.2023.11531931153193Targeted metabolomics reveals serum changes of amino acids in mild to moderate ischemic stroke and stroke mimicsShuxin Tao0Xinxing Xiao1Xin Li2Fan Na3Guo Na4Shuang Wang5Pin Zhang6Fang Hao7Peiran Zhao8Dong Guo9Xuewu Liu10Dawei Yang11Department of Neurology, Liaocheng People’s Hospital, Liaocheng, Shandong, ChinaDepartment of Neurology, Liaocheng People’s Hospital, Liaocheng, Shandong, ChinaDepartment of Clinical Laboratory, Zibo Central Hospital, Zibo, Shandong, ChinaZhong Yuan Academy of Biological Medicine, Liaocheng People’s Hospital, Liaocheng, ChinaExperimental Research Center, China Academy of Chinese Medical Sciences, Beijing, ChinaZhong Yuan Academy of Biological Medicine, Liaocheng People’s Hospital, Liaocheng, ChinaExperimental Research Center, China Academy of Chinese Medical Sciences, Beijing, ChinaDepartment of Neurology, Liaocheng People’s Hospital, Liaocheng, Shandong, ChinaZhong Yuan Academy of Biological Medicine, Liaocheng People’s Hospital, Liaocheng, ChinaDepartment of Neurology, Liaocheng People’s Hospital, Liaocheng, Shandong, ChinaDepartment of Neurology, Qilu Hospital of Shandong University, Institute of Epilepsy, Shandong University, Jinan, Shandong, ChinaZhong Yuan Academy of Biological Medicine, Liaocheng People’s Hospital, Liaocheng, ChinaBackgroundThe pathophysiological processes linked to an acute ischemic stroke (IS) can be reflected in the circulating metabolome. Amino acids (AAs) have been demonstrated to be one of the most significant metabolites that can undergo significant alteration after a stroke.MethodsWe sought to identify the potential biomarkers for the early detection of IS using an extensive targeted technique for reliable quantification of 27 different AAs based on ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). A cohort with 216 participants was enrolled, including 70 mild to moderate ischemic stroke patients (National Institutes of Health Stroke Scale < 15, MB group), 76 stroke mimics (MM group) and 70 healthy controls (NC group).ResultsIt was found that upon comparing MB and MM to control patients, AAs shifts were detected via partial least squares discrimination analysis (PLS-DA) and pathway analysis. Interestingly, MB and MM exhibited similar AAs pattern. Moreover, ornithine, asparagine, valine, citrulline, and cysteine were identified for inclusion in a biomarker panel for early-stage stroke detection based upon an AUC of 0.968 (95% CI 0.924–0.998). Levels of ornithine were positively associated with infract volume, 3 months mRS score, and National Institutes of Health Stroke Scale (NIHSS) score in MB. In addition, a metabolites biomarker panel, including ornithine, taurine, phenylalanine, citrulline, cysteine, yielded an AUC of 0.99 (95% CI 0.966–1) which can be employed to effectively discriminate MM patients from control.ConclusionOverall, alternations in serum AAs are characteristic metabolic features of MB and MM. AAs could serve as promising biomarkers for the early diagnosis of MB patients since mild to moderate IS patients were enrolled in the study. The metabolism of AAs can be considered as a key indicator for both the prevention and treatment of IS.https://www.frontiersin.org/articles/10.3389/fneur.2023.1153193/fullischemic strokestroke mimicstargeted metabolomicsamino acidsbiomarker |
| spellingShingle | Shuxin Tao Xinxing Xiao Xin Li Fan Na Guo Na Shuang Wang Pin Zhang Fang Hao Peiran Zhao Dong Guo Xuewu Liu Dawei Yang Targeted metabolomics reveals serum changes of amino acids in mild to moderate ischemic stroke and stroke mimics Frontiers in Neurology ischemic stroke stroke mimics targeted metabolomics amino acids biomarker |
| title | Targeted metabolomics reveals serum changes of amino acids in mild to moderate ischemic stroke and stroke mimics |
| title_full | Targeted metabolomics reveals serum changes of amino acids in mild to moderate ischemic stroke and stroke mimics |
| title_fullStr | Targeted metabolomics reveals serum changes of amino acids in mild to moderate ischemic stroke and stroke mimics |
| title_full_unstemmed | Targeted metabolomics reveals serum changes of amino acids in mild to moderate ischemic stroke and stroke mimics |
| title_short | Targeted metabolomics reveals serum changes of amino acids in mild to moderate ischemic stroke and stroke mimics |
| title_sort | targeted metabolomics reveals serum changes of amino acids in mild to moderate ischemic stroke and stroke mimics |
| topic | ischemic stroke stroke mimics targeted metabolomics amino acids biomarker |
| url | https://www.frontiersin.org/articles/10.3389/fneur.2023.1153193/full |
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