Molecular mechanism by which CDCP1 promotes proneural-mesenchymal transformation in primary glioblastoma
Abstract Background Compared with the proneural (PN) subtype of glioblastoma (GBM), the mesenchymal (MES) subtype is more invasive and immune evasive and is closely related to poor prognosis. Here, we used transcriptome data and experimental evidence to indicate that CUB domain-containing protein 1...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-04-01
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| Series: | Cancer Cell International |
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| Online Access: | https://doi.org/10.1186/s12935-021-02373-1 |
| _version_ | 1817990427538620416 |
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| author | Zhiying Lin Zhu Zhang Haojie Zheng Haiyan Xu Yajuan Wang Chao Chen Junlu Liu Guozhong Yi Zhiyong Li Xiaoyan Wang Guanglong Huang |
| author_facet | Zhiying Lin Zhu Zhang Haojie Zheng Haiyan Xu Yajuan Wang Chao Chen Junlu Liu Guozhong Yi Zhiyong Li Xiaoyan Wang Guanglong Huang |
| author_sort | Zhiying Lin |
| collection | DOAJ |
| description | Abstract Background Compared with the proneural (PN) subtype of glioblastoma (GBM), the mesenchymal (MES) subtype is more invasive and immune evasive and is closely related to poor prognosis. Here, we used transcriptome data and experimental evidence to indicate that CUB domain-containing protein 1 (CDCP1) is a novel regulator that facilitates the transformation of PN-GBM to MES-GBM. Methods The mRNA expression data of CDCP1 in glioma were collected from the TCGA, CGGA and GEO databases, and in vitro experiments verified CDCP1 expression in glioma tissue samples. Independent prognostic analysis revealed the correlation of the CDCP1 expression level and patient survival. Bioinformatics analysis and experiments verified the biological function of CDCP1. Multivariate proportional hazards models and a PPI network were used to select key genes. A prognostic risk model for predicting the survival of glioma patients was constructed based on the selected genes. Results The results showed that the expression of CDCP1 increased with increasing tumor grade and that the overexpression of CDCP1 correlated with a poor prognosis. CDCP1 was highly expressed in MES-GBM but weakly expressed in PN-GBM. The risk model (considering CDCP1 combined with CD44 and ITGAM expression) could represent a tool for predicting survival and prognosis in glioma patients. Conclusions Our study indicates that CDCP1 plays an important role in facilitating the transformation of PN-GBM to MES-GBM. |
| first_indexed | 2024-04-14T00:59:53Z |
| format | Article |
| id | doaj.art-f7f4771e91c24b12832deec2667ea24b |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-04-14T00:59:53Z |
| publishDate | 2022-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-f7f4771e91c24b12832deec2667ea24b2022-12-22T02:21:28ZengBMCCancer Cell International1475-28672022-04-0122111710.1186/s12935-021-02373-1Molecular mechanism by which CDCP1 promotes proneural-mesenchymal transformation in primary glioblastomaZhiying Lin0Zhu Zhang1Haojie Zheng2Haiyan Xu3Yajuan Wang4Chao Chen5Junlu Liu6Guozhong Yi7Zhiyong Li8Xiaoyan Wang9Guanglong Huang10Jiangxi Provincial People’s Hospital, The First Affiliated Hospital of Nanchang Medical CollegeDepartment of Neurosurgery, Nanfang Hospital, Southern Medical UniversityDepartment of Neurosurgery, Nanfang Hospital, Southern Medical UniversityDepartment of Neurosurgery, Nanfang Hospital, Southern Medical UniversityDepartment of Neurosurgery, Nanfang Hospital, Southern Medical UniversityDepartment of Neurosurgery, Nanfang Hospital, Southern Medical UniversityDepartment of Neurosurgery, Nanfang Hospital, Southern Medical UniversityDepartment of Neurosurgery, Nanfang Hospital, Southern Medical UniversityDepartment of Neurosurgery, Nanfang Hospital, Southern Medical UniversityDepartment of Neurosurgery, Nanfang Hospital, Southern Medical UniversityDepartment of Neurosurgery, Nanfang Hospital, Southern Medical UniversityAbstract Background Compared with the proneural (PN) subtype of glioblastoma (GBM), the mesenchymal (MES) subtype is more invasive and immune evasive and is closely related to poor prognosis. Here, we used transcriptome data and experimental evidence to indicate that CUB domain-containing protein 1 (CDCP1) is a novel regulator that facilitates the transformation of PN-GBM to MES-GBM. Methods The mRNA expression data of CDCP1 in glioma were collected from the TCGA, CGGA and GEO databases, and in vitro experiments verified CDCP1 expression in glioma tissue samples. Independent prognostic analysis revealed the correlation of the CDCP1 expression level and patient survival. Bioinformatics analysis and experiments verified the biological function of CDCP1. Multivariate proportional hazards models and a PPI network were used to select key genes. A prognostic risk model for predicting the survival of glioma patients was constructed based on the selected genes. Results The results showed that the expression of CDCP1 increased with increasing tumor grade and that the overexpression of CDCP1 correlated with a poor prognosis. CDCP1 was highly expressed in MES-GBM but weakly expressed in PN-GBM. The risk model (considering CDCP1 combined with CD44 and ITGAM expression) could represent a tool for predicting survival and prognosis in glioma patients. Conclusions Our study indicates that CDCP1 plays an important role in facilitating the transformation of PN-GBM to MES-GBM.https://doi.org/10.1186/s12935-021-02373-1GliomaCDCP1Proneural-mesenchymal transitionEpithelial-mesenchymal transitionPrognostic risk model |
| spellingShingle | Zhiying Lin Zhu Zhang Haojie Zheng Haiyan Xu Yajuan Wang Chao Chen Junlu Liu Guozhong Yi Zhiyong Li Xiaoyan Wang Guanglong Huang Molecular mechanism by which CDCP1 promotes proneural-mesenchymal transformation in primary glioblastoma Cancer Cell International Glioma CDCP1 Proneural-mesenchymal transition Epithelial-mesenchymal transition Prognostic risk model |
| title | Molecular mechanism by which CDCP1 promotes proneural-mesenchymal transformation in primary glioblastoma |
| title_full | Molecular mechanism by which CDCP1 promotes proneural-mesenchymal transformation in primary glioblastoma |
| title_fullStr | Molecular mechanism by which CDCP1 promotes proneural-mesenchymal transformation in primary glioblastoma |
| title_full_unstemmed | Molecular mechanism by which CDCP1 promotes proneural-mesenchymal transformation in primary glioblastoma |
| title_short | Molecular mechanism by which CDCP1 promotes proneural-mesenchymal transformation in primary glioblastoma |
| title_sort | molecular mechanism by which cdcp1 promotes proneural mesenchymal transformation in primary glioblastoma |
| topic | Glioma CDCP1 Proneural-mesenchymal transition Epithelial-mesenchymal transition Prognostic risk model |
| url | https://doi.org/10.1186/s12935-021-02373-1 |
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