Study on the Incorporation of Chitosan Flakes in Electrospun Polycaprolactone Scaffolds
Hybrid scaffolds obtained by combining two or more biopolymers are studied in the context of tissue regeneration due to the possibility of achieving new functional properties or structural features. The aim of this work was to produce a new type of hybrid polycaprolactone (PCL)/chitosan (CS) electro...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/8/1496 |
| _version_ | 1797444135867645952 |
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| author | Diana Querido Tânia Vieira José Luís Ferreira Célia Henriques João Paulo Borges Jorge Carvalho Silva |
| author_facet | Diana Querido Tânia Vieira José Luís Ferreira Célia Henriques João Paulo Borges Jorge Carvalho Silva |
| author_sort | Diana Querido |
| collection | DOAJ |
| description | Hybrid scaffolds obtained by combining two or more biopolymers are studied in the context of tissue regeneration due to the possibility of achieving new functional properties or structural features. The aim of this work was to produce a new type of hybrid polycaprolactone (PCL)/chitosan (CS) electrospun mat through the controlled deposition of CS flakes interspaced between the PCL fibers. A poly(ethylene oxide) (PEO) solution was used to transport CS flakes with controlled size. This, and the PCL solution, were simultaneously electrospun onto a rotatory mandrel in a perpendicular setup. Different PCL/CS mass ratios were also studied. The morphology of the resulting fibers, evaluated by SEM, confirmed the presence of the CS flakes between the PCL fibers. The addition of PEO/CS fibers resulted in hydrophilic mats with lower Young’s modulus relatively to PCL mats. In vitro cell culture results indicated that the addition of CS lowers both the adhesion and the proliferation of human dermal fibroblasts. The present work demonstrates the feasibility of achieving a controlled deposition of a polymeric component in granular form onto a collector where electrospun nanofibers are being deposited, thereby producing a hybrid scaffold. |
| first_indexed | 2024-03-09T13:07:14Z |
| format | Article |
| id | doaj.art-f7f4fb7b509840b49fc58c9e2d66bc32 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T13:07:14Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-f7f4fb7b509840b49fc58c9e2d66bc322023-11-30T21:46:55ZengMDPI AGPolymers2073-43602022-04-01148149610.3390/polym14081496Study on the Incorporation of Chitosan Flakes in Electrospun Polycaprolactone ScaffoldsDiana Querido0Tânia Vieira1José Luís Ferreira2Célia Henriques3João Paulo Borges4Jorge Carvalho Silva5Cenimat/I3N, Physics Department, School of Science and Technology, Universidade Nova de Lisboa, Caparica, 2819-516 Almada, PortugalCenimat/I3N, Physics Department, School of Science and Technology, Universidade Nova de Lisboa, Caparica, 2819-516 Almada, PortugalCenimat/I3N, Physics Department, School of Science and Technology, Universidade Nova de Lisboa, Caparica, 2819-516 Almada, PortugalCenimat/I3N, Physics Department, School of Science and Technology, Universidade Nova de Lisboa, Caparica, 2819-516 Almada, PortugalCenimat/I3N, Materials Science Department, School of Science and Technology, Universidade Nova de Lisboa, Caparica, 2819-516 Almada, PortugalCenimat/I3N, Physics Department, School of Science and Technology, Universidade Nova de Lisboa, Caparica, 2819-516 Almada, PortugalHybrid scaffolds obtained by combining two or more biopolymers are studied in the context of tissue regeneration due to the possibility of achieving new functional properties or structural features. The aim of this work was to produce a new type of hybrid polycaprolactone (PCL)/chitosan (CS) electrospun mat through the controlled deposition of CS flakes interspaced between the PCL fibers. A poly(ethylene oxide) (PEO) solution was used to transport CS flakes with controlled size. This, and the PCL solution, were simultaneously electrospun onto a rotatory mandrel in a perpendicular setup. Different PCL/CS mass ratios were also studied. The morphology of the resulting fibers, evaluated by SEM, confirmed the presence of the CS flakes between the PCL fibers. The addition of PEO/CS fibers resulted in hydrophilic mats with lower Young’s modulus relatively to PCL mats. In vitro cell culture results indicated that the addition of CS lowers both the adhesion and the proliferation of human dermal fibroblasts. The present work demonstrates the feasibility of achieving a controlled deposition of a polymeric component in granular form onto a collector where electrospun nanofibers are being deposited, thereby producing a hybrid scaffold.https://www.mdpi.com/2073-4360/14/8/1496hybrid scaffoldstissue engineeringco-electrospinningchitosanpolycaprolactonefibroblasts |
| spellingShingle | Diana Querido Tânia Vieira José Luís Ferreira Célia Henriques João Paulo Borges Jorge Carvalho Silva Study on the Incorporation of Chitosan Flakes in Electrospun Polycaprolactone Scaffolds Polymers hybrid scaffolds tissue engineering co-electrospinning chitosan polycaprolactone fibroblasts |
| title | Study on the Incorporation of Chitosan Flakes in Electrospun Polycaprolactone Scaffolds |
| title_full | Study on the Incorporation of Chitosan Flakes in Electrospun Polycaprolactone Scaffolds |
| title_fullStr | Study on the Incorporation of Chitosan Flakes in Electrospun Polycaprolactone Scaffolds |
| title_full_unstemmed | Study on the Incorporation of Chitosan Flakes in Electrospun Polycaprolactone Scaffolds |
| title_short | Study on the Incorporation of Chitosan Flakes in Electrospun Polycaprolactone Scaffolds |
| title_sort | study on the incorporation of chitosan flakes in electrospun polycaprolactone scaffolds |
| topic | hybrid scaffolds tissue engineering co-electrospinning chitosan polycaprolactone fibroblasts |
| url | https://www.mdpi.com/2073-4360/14/8/1496 |
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