Species diversity of thrips (Thysanoptera) in selected avocado orchards from Mexico based on morphology and molecular data
Avocado is one of the most important crops in the world, and Mexico is the largest producer of this fruit. Several insect pests affect its production, and thrips are amongst the most important. A key step in the design of control methods is accurate species identification. Despite this, formal repor...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2018-11-01
|
| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311918620441 |
| _version_ | 1818839034160480256 |
|---|---|
| author | Daniel Bravo-Pérez Ma. Teresa Santillán-Galicia Roberto M. Johansen-Naime Héctor González-Hernández Obdulia L. Segura-León Daniel L. Ochoa-Martínez Stephanie Guzman-Valencia |
| author_facet | Daniel Bravo-Pérez Ma. Teresa Santillán-Galicia Roberto M. Johansen-Naime Héctor González-Hernández Obdulia L. Segura-León Daniel L. Ochoa-Martínez Stephanie Guzman-Valencia |
| author_sort | Daniel Bravo-Pérez |
| collection | DOAJ |
| description | Avocado is one of the most important crops in the world, and Mexico is the largest producer of this fruit. Several insect pests affect its production, and thrips are amongst the most important. A key step in the design of control methods is accurate species identification. Despite this, formal reports on species diversity of thrips in Mexico are very scarce. Morphological identification can sometimes be time-consuming and inconclusive. Therefore, we explored the species diversity of thrips in Mexican avocado orchards (Michoacan state) based on partial sequences of the mitochondrial gene cytochrome oxidase subunit I (COI). Forty-four specimens were analysed, which represented approximately 8% of all individuals collected from five localities distributed in three Municipalities. All specimens were analysed using the COI marker, and specimens within the genera Frankliniella were also analysed using a marker within the D2 domain of the 28S (28SD2) nuclear ribosomal DNA. Molecular identifications were confirmed using morphological taxonomy. Overall, six genera were found (Neohydatothrips, Scirtothrips, Frankliniella, Arorathrips, Caliothrips and Leptothrips). All genera contained only one species, except Frankliniella, for which there were six species. Data from the two molecular markers suggest the existence of cryptic species within Mexican F. occidentalis populations. |
| first_indexed | 2024-12-19T03:47:52Z |
| format | Article |
| id | doaj.art-f7f66728196b4f91806a536d3ea1e1ae |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-19T03:47:52Z |
| publishDate | 2018-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-f7f66728196b4f91806a536d3ea1e1ae2022-12-21T20:37:05ZengElsevierJournal of Integrative Agriculture2095-31192018-11-01171125092517Species diversity of thrips (Thysanoptera) in selected avocado orchards from Mexico based on morphology and molecular dataDaniel Bravo-Pérez0Ma. Teresa Santillán-Galicia1Roberto M. Johansen-Naime2Héctor González-Hernández3Obdulia L. Segura-León4Daniel L. Ochoa-Martínez5Stephanie Guzman-Valencia6Posgrado en Fitosanidad-Entomología y Acarología, Colegio de Postgraduados, Km 36.5 Carretera México-Texcoco, Montecillo, Texcoco, Estado de México 56230, MéxicoPosgrado en Fitosanidad-Entomología y Acarología, Colegio de Postgraduados, Km 36.5 Carretera México-Texcoco, Montecillo, Texcoco, Estado de México 56230, MéxicoInstituto de Biología de la Universidad Nacional Autónoma de México (IBUNAM), Circuito exterior s/n, Ciudad Universitaria, Coyoacán, Ciudad de México 04510, MéxicoPosgrado en Fitosanidad-Entomología y Acarología, Colegio de Postgraduados, Km 36.5 Carretera México-Texcoco, Montecillo, Texcoco, Estado de México 56230, México; Correspondence Héctor González-Hernández, Tel: +52-595-9520200 (Ext. 1667), Fax: +52-555-8045967Posgrado en Fitosanidad-Entomología y Acarología, Colegio de Postgraduados, Km 36.5 Carretera México-Texcoco, Montecillo, Texcoco, Estado de México 56230, MéxicoPosgrado en Fitosanidad-Fitopatología, Colegio de Postgraduados, Km 36.5 Carretera México-Texcoco, Montecillo, Texcoco, Estado de México 56230, MéxicoPosgrado en Fitosanidad-Entomología y Acarología, Colegio de Postgraduados, Km 36.5 Carretera México-Texcoco, Montecillo, Texcoco, Estado de México 56230, MéxicoAvocado is one of the most important crops in the world, and Mexico is the largest producer of this fruit. Several insect pests affect its production, and thrips are amongst the most important. A key step in the design of control methods is accurate species identification. Despite this, formal reports on species diversity of thrips in Mexico are very scarce. Morphological identification can sometimes be time-consuming and inconclusive. Therefore, we explored the species diversity of thrips in Mexican avocado orchards (Michoacan state) based on partial sequences of the mitochondrial gene cytochrome oxidase subunit I (COI). Forty-four specimens were analysed, which represented approximately 8% of all individuals collected from five localities distributed in three Municipalities. All specimens were analysed using the COI marker, and specimens within the genera Frankliniella were also analysed using a marker within the D2 domain of the 28S (28SD2) nuclear ribosomal DNA. Molecular identifications were confirmed using morphological taxonomy. Overall, six genera were found (Neohydatothrips, Scirtothrips, Frankliniella, Arorathrips, Caliothrips and Leptothrips). All genera contained only one species, except Frankliniella, for which there were six species. Data from the two molecular markers suggest the existence of cryptic species within Mexican F. occidentalis populations.http://www.sciencedirect.com/science/article/pii/S2095311918620441molecular taxonomymorphological identificationcytochrome oxidase subunit I28S nuclear ribosomal DNA |
| spellingShingle | Daniel Bravo-Pérez Ma. Teresa Santillán-Galicia Roberto M. Johansen-Naime Héctor González-Hernández Obdulia L. Segura-León Daniel L. Ochoa-Martínez Stephanie Guzman-Valencia Species diversity of thrips (Thysanoptera) in selected avocado orchards from Mexico based on morphology and molecular data Journal of Integrative Agriculture molecular taxonomy morphological identification cytochrome oxidase subunit I 28S nuclear ribosomal DNA |
| title | Species diversity of thrips (Thysanoptera) in selected avocado orchards from Mexico based on morphology and molecular data |
| title_full | Species diversity of thrips (Thysanoptera) in selected avocado orchards from Mexico based on morphology and molecular data |
| title_fullStr | Species diversity of thrips (Thysanoptera) in selected avocado orchards from Mexico based on morphology and molecular data |
| title_full_unstemmed | Species diversity of thrips (Thysanoptera) in selected avocado orchards from Mexico based on morphology and molecular data |
| title_short | Species diversity of thrips (Thysanoptera) in selected avocado orchards from Mexico based on morphology and molecular data |
| title_sort | species diversity of thrips thysanoptera in selected avocado orchards from mexico based on morphology and molecular data |
| topic | molecular taxonomy morphological identification cytochrome oxidase subunit I 28S nuclear ribosomal DNA |
| url | http://www.sciencedirect.com/science/article/pii/S2095311918620441 |
| work_keys_str_mv | AT danielbravoperez speciesdiversityofthripsthysanopterainselectedavocadoorchardsfrommexicobasedonmorphologyandmoleculardata AT materesasantillangalicia speciesdiversityofthripsthysanopterainselectedavocadoorchardsfrommexicobasedonmorphologyandmoleculardata AT robertomjohansennaime speciesdiversityofthripsthysanopterainselectedavocadoorchardsfrommexicobasedonmorphologyandmoleculardata AT hectorgonzalezhernandez speciesdiversityofthripsthysanopterainselectedavocadoorchardsfrommexicobasedonmorphologyandmoleculardata AT obdulialseguraleon speciesdiversityofthripsthysanopterainselectedavocadoorchardsfrommexicobasedonmorphologyandmoleculardata AT daniellochoamartinez speciesdiversityofthripsthysanopterainselectedavocadoorchardsfrommexicobasedonmorphologyandmoleculardata AT stephanieguzmanvalencia speciesdiversityofthripsthysanopterainselectedavocadoorchardsfrommexicobasedonmorphologyandmoleculardata |