Anti-Cancer Effects of Glaucarubinone in the Hepatocellular Carcinoma Cell Line Huh7 via Regulation of the Epithelial-To-Mesenchymal Transition-Associated Transcription Factor Twist1
Hepatocellular carcinoma (HCC), the most common type of liver cancer, is a leading cause of cancer-related deaths. As HCC has a high mortality rate and its incidence is increasing worldwide, understanding and treating HCC are crucial for resolving major public health concerns. In the present study,...
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MDPI AG
2021-02-01
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| Series: | International Journal of Molecular Sciences |
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| author | Jihye Seo Jain Ha Eunjeong Kang Haelim Yoon Sewoong Lee Shi Yong Ryu Kwonseop Kim Sayeon Cho |
| author_facet | Jihye Seo Jain Ha Eunjeong Kang Haelim Yoon Sewoong Lee Shi Yong Ryu Kwonseop Kim Sayeon Cho |
| author_sort | Jihye Seo |
| collection | DOAJ |
| description | Hepatocellular carcinoma (HCC), the most common type of liver cancer, is a leading cause of cancer-related deaths. As HCC has a high mortality rate and its incidence is increasing worldwide, understanding and treating HCC are crucial for resolving major public health concerns. In the present study, wound healing screening assays were performed using natural product libraries to identify natural chemicals that can inhibit cancer cell migration. Glaucarubinone (GCB) showed a high potential for inhibiting cell migration. The anti-cancer effects of GCB were evaluated using the HCC cell line, Huh7. GCB showed anti-cancer effects, as verified by wound healing, cell migration, invasion, colony formation, and three-dimensional spheroid invasion assays. In addition, cells treated with GCB showed suppressed matrix metalloproteinase activities. Immunoblotting analyses of intracellular signaling pathways revealed that GCB regulated the levels of Twist1, a crucial transcription factor associated with epithelial-to-mesenchymal transition, and mitogen-activated protein kinase. The invasive ability of cancer cells was found to be decreased by the regulation of Twist1 protein levels. Furthermore, GCB downregulated phosphorylation of extracellular signal-regulated kinase. These results indicate that GCB exhibits anti-metastatic properties in Huh7 cells, suggesting that it could be used to treat HCC. |
| first_indexed | 2024-03-09T05:06:34Z |
| format | Article |
| id | doaj.art-f7f70a3ea71e41f3ab12bd3e9b36cbc7 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T05:06:34Z |
| publishDate | 2021-02-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f7f70a3ea71e41f3ab12bd3e9b36cbc72023-12-03T12:54:24ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-02-01224170010.3390/ijms22041700Anti-Cancer Effects of Glaucarubinone in the Hepatocellular Carcinoma Cell Line Huh7 via Regulation of the Epithelial-To-Mesenchymal Transition-Associated Transcription Factor Twist1Jihye Seo0Jain Ha1Eunjeong Kang2Haelim Yoon3Sewoong Lee4Shi Yong Ryu5Kwonseop Kim6Sayeon Cho7Laboratory of Molecular and Pharmacological Cell Biology, College of Pharmacy, Chung-Ang University, Seoul 06974, KoreaLaboratory of Molecular and Pharmacological Cell Biology, College of Pharmacy, Chung-Ang University, Seoul 06974, KoreaLaboratory of Molecular and Pharmacological Cell Biology, College of Pharmacy, Chung-Ang University, Seoul 06974, KoreaLaboratory of Molecular and Pharmacological Cell Biology, College of Pharmacy, Chung-Ang University, Seoul 06974, KoreaLaboratory of Molecular and Pharmacological Cell Biology, College of Pharmacy, Chung-Ang University, Seoul 06974, KoreaKorea Research Institute of Chemical Technology (KRICT), Daejeon 34114, KoreaCollege of Pharmacy and Research Institute for Drug Development, Chonnam National University, Gwangju 61186, KoreaLaboratory of Molecular and Pharmacological Cell Biology, College of Pharmacy, Chung-Ang University, Seoul 06974, KoreaHepatocellular carcinoma (HCC), the most common type of liver cancer, is a leading cause of cancer-related deaths. As HCC has a high mortality rate and its incidence is increasing worldwide, understanding and treating HCC are crucial for resolving major public health concerns. In the present study, wound healing screening assays were performed using natural product libraries to identify natural chemicals that can inhibit cancer cell migration. Glaucarubinone (GCB) showed a high potential for inhibiting cell migration. The anti-cancer effects of GCB were evaluated using the HCC cell line, Huh7. GCB showed anti-cancer effects, as verified by wound healing, cell migration, invasion, colony formation, and three-dimensional spheroid invasion assays. In addition, cells treated with GCB showed suppressed matrix metalloproteinase activities. Immunoblotting analyses of intracellular signaling pathways revealed that GCB regulated the levels of Twist1, a crucial transcription factor associated with epithelial-to-mesenchymal transition, and mitogen-activated protein kinase. The invasive ability of cancer cells was found to be decreased by the regulation of Twist1 protein levels. Furthermore, GCB downregulated phosphorylation of extracellular signal-regulated kinase. These results indicate that GCB exhibits anti-metastatic properties in Huh7 cells, suggesting that it could be used to treat HCC.https://www.mdpi.com/1422-0067/22/4/1700glaucarubinoneTwist1epithelial-to-mesenchymal transitionanti-cancer |
| spellingShingle | Jihye Seo Jain Ha Eunjeong Kang Haelim Yoon Sewoong Lee Shi Yong Ryu Kwonseop Kim Sayeon Cho Anti-Cancer Effects of Glaucarubinone in the Hepatocellular Carcinoma Cell Line Huh7 via Regulation of the Epithelial-To-Mesenchymal Transition-Associated Transcription Factor Twist1 International Journal of Molecular Sciences glaucarubinone Twist1 epithelial-to-mesenchymal transition anti-cancer |
| title | Anti-Cancer Effects of Glaucarubinone in the Hepatocellular Carcinoma Cell Line Huh7 via Regulation of the Epithelial-To-Mesenchymal Transition-Associated Transcription Factor Twist1 |
| title_full | Anti-Cancer Effects of Glaucarubinone in the Hepatocellular Carcinoma Cell Line Huh7 via Regulation of the Epithelial-To-Mesenchymal Transition-Associated Transcription Factor Twist1 |
| title_fullStr | Anti-Cancer Effects of Glaucarubinone in the Hepatocellular Carcinoma Cell Line Huh7 via Regulation of the Epithelial-To-Mesenchymal Transition-Associated Transcription Factor Twist1 |
| title_full_unstemmed | Anti-Cancer Effects of Glaucarubinone in the Hepatocellular Carcinoma Cell Line Huh7 via Regulation of the Epithelial-To-Mesenchymal Transition-Associated Transcription Factor Twist1 |
| title_short | Anti-Cancer Effects of Glaucarubinone in the Hepatocellular Carcinoma Cell Line Huh7 via Regulation of the Epithelial-To-Mesenchymal Transition-Associated Transcription Factor Twist1 |
| title_sort | anti cancer effects of glaucarubinone in the hepatocellular carcinoma cell line huh7 via regulation of the epithelial to mesenchymal transition associated transcription factor twist1 |
| topic | glaucarubinone Twist1 epithelial-to-mesenchymal transition anti-cancer |
| url | https://www.mdpi.com/1422-0067/22/4/1700 |
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