Screening and Verification of Key Genes for Isomaltulose Reducing Liver Fat Accumulation in Mice
In order to screen and verify the key genes that isomaltulose reduces liver fat accumulation in mice, in this study, the Limma package was used to analyze the liver transcriptome data of male mice fed with isomaltose and sucrose for 22 weeks (GSE54723, n=14). A total of 49 DEGs were screened out, an...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
The editorial department of Science and Technology of Food Industry
2022-08-01
|
| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021110090 |
| _version_ | 1798017619852263424 |
|---|---|
| author | Qiuling HE Caiping ZHANG Jing GUI Yongxing LIU Jiuxiu JI Bo ZHOU Zhen ZHANG |
| author_facet | Qiuling HE Caiping ZHANG Jing GUI Yongxing LIU Jiuxiu JI Bo ZHOU Zhen ZHANG |
| author_sort | Qiuling HE |
| collection | DOAJ |
| description | In order to screen and verify the key genes that isomaltulose reduces liver fat accumulation in mice, in this study, the Limma package was used to analyze the liver transcriptome data of male mice fed with isomaltose and sucrose for 22 weeks (GSE54723, n=14). A total of 49 DEGs were screened out, and then were analyzed by Metascape software for GO functional enrichment analysis and KEGG signaling pathways analysis. Cytoscape and WGCNA software were used to construct gene co-expression network, overlapping genes were picked up for searching for key node genes, and 10 key node genes Pnliprp1, Prss2, Clps, Tff2, Pnlip, Ctrl, Cpa1, Cel, Dmbt1, Vil1 were screened out, gene functional analysis showed Pnlip, Pnliprp1, Cel, Clps were related to lipid metabolism. The qPCR results showed that the expression of these four genes related to lipid metabolism in liver tissue of isomaltulose group was significantly (P<0.05) higher than that of sucrose group, by comparing the expression of the above genes in the liver transcriptome data of normal people and NAFLD patients (GSE163211, n=318), Pnlip, Pnliprp1, Cel and Clps were also expressed significantly higher in normal people than in NAFLD patients. This study revealed that isomaltulose promoted lipolysis by up regulating the synthesis of Pnlip, Pnliprp1, Cel and Clps, so as to reduce the accumulation of liver lipids, which would provide a theoretical support for the study of the molecular mechanism of isomaltulose affecting liver lipid metabolism. |
| first_indexed | 2024-04-11T16:10:17Z |
| format | Article |
| id | doaj.art-f7f7307a580548f6a7a306a7c1bc4510 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-11T16:10:17Z |
| publishDate | 2022-08-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-f7f7307a580548f6a7a306a7c1bc45102022-12-22T04:14:43ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-08-0143161810.13386/j.issn1002-0306.20211100902021110090-16Screening and Verification of Key Genes for Isomaltulose Reducing Liver Fat Accumulation in MiceQiuling HE0Caiping ZHANG1Jing GUI2Yongxing LIU3Jiuxiu JI4Bo ZHOU5Zhen ZHANG6College of Pharmacy, Guilin Medical University, Guilin 541199, ChinaCollege of Intelligent Medicine and Biotechnology, Guilin Medical University, Guilin 541199, ChinaCollege of Intelligent Medicine and Biotechnology, Guilin Medical University, Guilin 541199, ChinaCollege of Intelligent Medicine and Biotechnology, Guilin Medical University, Guilin 541199, ChinaCollege of Agriculture and Forestry, Linyi University, Linyi 276005, ChinaScientific Research Center, Guilin Medical University, Guilin 541199, ChinaCollege of Intelligent Medicine and Biotechnology, Guilin Medical University, Guilin 541199, ChinaIn order to screen and verify the key genes that isomaltulose reduces liver fat accumulation in mice, in this study, the Limma package was used to analyze the liver transcriptome data of male mice fed with isomaltose and sucrose for 22 weeks (GSE54723, n=14). A total of 49 DEGs were screened out, and then were analyzed by Metascape software for GO functional enrichment analysis and KEGG signaling pathways analysis. Cytoscape and WGCNA software were used to construct gene co-expression network, overlapping genes were picked up for searching for key node genes, and 10 key node genes Pnliprp1, Prss2, Clps, Tff2, Pnlip, Ctrl, Cpa1, Cel, Dmbt1, Vil1 were screened out, gene functional analysis showed Pnlip, Pnliprp1, Cel, Clps were related to lipid metabolism. The qPCR results showed that the expression of these four genes related to lipid metabolism in liver tissue of isomaltulose group was significantly (P<0.05) higher than that of sucrose group, by comparing the expression of the above genes in the liver transcriptome data of normal people and NAFLD patients (GSE163211, n=318), Pnlip, Pnliprp1, Cel and Clps were also expressed significantly higher in normal people than in NAFLD patients. This study revealed that isomaltulose promoted lipolysis by up regulating the synthesis of Pnlip, Pnliprp1, Cel and Clps, so as to reduce the accumulation of liver lipids, which would provide a theoretical support for the study of the molecular mechanism of isomaltulose affecting liver lipid metabolism.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021110090isomaltulosesucrosetranscriptome datanode geneslipid metabolism |
| spellingShingle | Qiuling HE Caiping ZHANG Jing GUI Yongxing LIU Jiuxiu JI Bo ZHOU Zhen ZHANG Screening and Verification of Key Genes for Isomaltulose Reducing Liver Fat Accumulation in Mice Shipin gongye ke-ji isomaltulose sucrose transcriptome data node genes lipid metabolism |
| title | Screening and Verification of Key Genes for Isomaltulose Reducing Liver Fat Accumulation in Mice |
| title_full | Screening and Verification of Key Genes for Isomaltulose Reducing Liver Fat Accumulation in Mice |
| title_fullStr | Screening and Verification of Key Genes for Isomaltulose Reducing Liver Fat Accumulation in Mice |
| title_full_unstemmed | Screening and Verification of Key Genes for Isomaltulose Reducing Liver Fat Accumulation in Mice |
| title_short | Screening and Verification of Key Genes for Isomaltulose Reducing Liver Fat Accumulation in Mice |
| title_sort | screening and verification of key genes for isomaltulose reducing liver fat accumulation in mice |
| topic | isomaltulose sucrose transcriptome data node genes lipid metabolism |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021110090 |
| work_keys_str_mv | AT qiulinghe screeningandverificationofkeygenesforisomaltulosereducingliverfataccumulationinmice AT caipingzhang screeningandverificationofkeygenesforisomaltulosereducingliverfataccumulationinmice AT jinggui screeningandverificationofkeygenesforisomaltulosereducingliverfataccumulationinmice AT yongxingliu screeningandverificationofkeygenesforisomaltulosereducingliverfataccumulationinmice AT jiuxiuji screeningandverificationofkeygenesforisomaltulosereducingliverfataccumulationinmice AT bozhou screeningandverificationofkeygenesforisomaltulosereducingliverfataccumulationinmice AT zhenzhang screeningandverificationofkeygenesforisomaltulosereducingliverfataccumulationinmice |