Direct Cloning of a Xylanase Gene from Pawan-Riau Hot Spring
A functional gene containing an Open Reading Frame (ORF) encoding a -1, 4-endoxylanase glycosyl hydrolase family 11 was cloned directly using metagenomic PCR-cloning method from Pawan Hot Spring sample in Riau. The gene consisted of 642 nucleotides, encoded for 213 amino acids. The amino acid seque...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2007-06-01
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| Series: | Hayati Journal of Biosciences |
| Subjects: | |
| Online Access: | http://journal.ipb.ac.id/index.php/hayati/article/viewFile/239/105 |
| _version_ | 1828778061034684416 |
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| author | IS HELIANTI |
| author_facet | IS HELIANTI |
| author_sort | IS HELIANTI |
| collection | DOAJ |
| description | A functional gene containing an Open Reading Frame (ORF) encoding a -1, 4-endoxylanase glycosyl hydrolase family 11 was cloned directly using metagenomic PCR-cloning method from Pawan Hot Spring sample in Riau. The gene consisted of 642 nucleotides, encoded for 213 amino acids. The amino acid sequence analysis using BLAST showed that the gene has high homology (93%) with xylanase gene from Bacillus subtilis. The gene showed its function when it was subcloned into an expression vector and overexpressed in E. coli. The crude extract of the recombinant enzyme had activity for 170 U/ml at 50 oC. The result of this work showed that metagenomic approach was a powerful short cut method to obtain recombinant biocatalyst that was useful for industrial application. |
| first_indexed | 2024-12-11T16:34:39Z |
| format | Article |
| id | doaj.art-f7fb3c403f0148718519685006cda142 |
| institution | Directory Open Access Journal |
| issn | 1978-3019 2086-4094 |
| language | English |
| last_indexed | 2024-12-11T16:34:39Z |
| publishDate | 2007-06-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-f7fb3c403f0148718519685006cda1422022-12-22T00:58:30ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942007-06-011425458Direct Cloning of a Xylanase Gene from Pawan-Riau Hot SpringIS HELIANTIA functional gene containing an Open Reading Frame (ORF) encoding a -1, 4-endoxylanase glycosyl hydrolase family 11 was cloned directly using metagenomic PCR-cloning method from Pawan Hot Spring sample in Riau. The gene consisted of 642 nucleotides, encoded for 213 amino acids. The amino acid sequence analysis using BLAST showed that the gene has high homology (93%) with xylanase gene from Bacillus subtilis. The gene showed its function when it was subcloned into an expression vector and overexpressed in E. coli. The crude extract of the recombinant enzyme had activity for 170 U/ml at 50 oC. The result of this work showed that metagenomic approach was a powerful short cut method to obtain recombinant biocatalyst that was useful for industrial application.http://journal.ipb.ac.id/index.php/hayati/article/viewFile/239/10514-endoxylanasemetagenomic DNAPawan-Riau hot-spring |
| spellingShingle | IS HELIANTI Direct Cloning of a Xylanase Gene from Pawan-Riau Hot Spring Hayati Journal of Biosciences 1 4-endoxylanase metagenomic DNA Pawan-Riau hot-spring |
| title | Direct Cloning of a Xylanase Gene from Pawan-Riau Hot Spring |
| title_full | Direct Cloning of a Xylanase Gene from Pawan-Riau Hot Spring |
| title_fullStr | Direct Cloning of a Xylanase Gene from Pawan-Riau Hot Spring |
| title_full_unstemmed | Direct Cloning of a Xylanase Gene from Pawan-Riau Hot Spring |
| title_short | Direct Cloning of a Xylanase Gene from Pawan-Riau Hot Spring |
| title_sort | direct cloning of a xylanase gene from pawan riau hot spring |
| topic | 1 4-endoxylanase metagenomic DNA Pawan-Riau hot-spring |
| url | http://journal.ipb.ac.id/index.php/hayati/article/viewFile/239/105 |
| work_keys_str_mv | AT ishelianti directcloningofaxylanasegenefrompawanriauhotspring |