Metabolomic Profiling Reveals Distinct and Mutual Effects of Diet and Inflammation in Shaping Systemic Metabolism in <i>Ldlr<sup>−/−</sup></i> Mice
Changes in modern dietary habits such as consumption of Western-type diets affect physiology on several levels, including metabolism and inflammation. It is currently unclear whether changes in systemic metabolism due to dietary interventions are long-lasting and affect acute inflammatory processes....
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/10/9/336 |
| _version_ | 1827708997620203520 |
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| author | Mario A. Lauterbach Eicke Latz Anette Christ |
| author_facet | Mario A. Lauterbach Eicke Latz Anette Christ |
| author_sort | Mario A. Lauterbach |
| collection | DOAJ |
| description | Changes in modern dietary habits such as consumption of Western-type diets affect physiology on several levels, including metabolism and inflammation. It is currently unclear whether changes in systemic metabolism due to dietary interventions are long-lasting and affect acute inflammatory processes. Here, we investigated how high-fat diet (HFD) feeding altered systemic metabolism and the metabolomic response to inflammatory stimuli. We conducted metabolomic profiling of sera collected from <i>Ldlr</i><sup>−/−</sup> mice on either regular chow diet (CD) or HFD, and after an additional low-dose lipopolysaccharide (LPS) challenge. HFD feeding, as well as LPS treatment, elicited pronounced metabolic changes. HFD qualitatively altered the systemic metabolic response to LPS; particularly, serum concentrations of fatty acids and their metabolites varied between LPS-challenged mice on HFD or CD, respectively. To investigate whether systemic metabolic changes were sustained long-term, mice fed HFD were shifted back to CD after four weeks (HFD > CD). When shifted back to CD, serum metabolites returned to baseline levels, and so did the response to LPS. Our results imply that systemic metabolism rapidly adapts to dietary changes. The profound systemic metabolic rewiring observed in response to diet might affect immune cell reprogramming and inflammatory responses. |
| first_indexed | 2024-03-10T17:13:44Z |
| format | Article |
| id | doaj.art-f7fb449bac2245129063a06a99e45ccd |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T17:13:44Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-f7fb449bac2245129063a06a99e45ccd2023-11-20T10:34:49ZengMDPI AGMetabolites2218-19892020-08-0110933610.3390/metabo10090336Metabolomic Profiling Reveals Distinct and Mutual Effects of Diet and Inflammation in Shaping Systemic Metabolism in <i>Ldlr<sup>−/−</sup></i> MiceMario A. Lauterbach0Eicke Latz1Anette Christ2Institute of Innate Immunity, University Hospital Bonn, University of Bonn, 53127 Bonn, GermanyInstitute of Innate Immunity, University Hospital Bonn, University of Bonn, 53127 Bonn, GermanyInstitute of Innate Immunity, University Hospital Bonn, University of Bonn, 53127 Bonn, GermanyChanges in modern dietary habits such as consumption of Western-type diets affect physiology on several levels, including metabolism and inflammation. It is currently unclear whether changes in systemic metabolism due to dietary interventions are long-lasting and affect acute inflammatory processes. Here, we investigated how high-fat diet (HFD) feeding altered systemic metabolism and the metabolomic response to inflammatory stimuli. We conducted metabolomic profiling of sera collected from <i>Ldlr</i><sup>−/−</sup> mice on either regular chow diet (CD) or HFD, and after an additional low-dose lipopolysaccharide (LPS) challenge. HFD feeding, as well as LPS treatment, elicited pronounced metabolic changes. HFD qualitatively altered the systemic metabolic response to LPS; particularly, serum concentrations of fatty acids and their metabolites varied between LPS-challenged mice on HFD or CD, respectively. To investigate whether systemic metabolic changes were sustained long-term, mice fed HFD were shifted back to CD after four weeks (HFD > CD). When shifted back to CD, serum metabolites returned to baseline levels, and so did the response to LPS. Our results imply that systemic metabolism rapidly adapts to dietary changes. The profound systemic metabolic rewiring observed in response to diet might affect immune cell reprogramming and inflammatory responses.https://www.mdpi.com/2218-1989/10/9/336western-type dietslipopolysaccharide (LPS)systemic metabolismsystemic inflammationmetabolomic profilinglong-term metabolic rewiring |
| spellingShingle | Mario A. Lauterbach Eicke Latz Anette Christ Metabolomic Profiling Reveals Distinct and Mutual Effects of Diet and Inflammation in Shaping Systemic Metabolism in <i>Ldlr<sup>−/−</sup></i> Mice Metabolites western-type diets lipopolysaccharide (LPS) systemic metabolism systemic inflammation metabolomic profiling long-term metabolic rewiring |
| title | Metabolomic Profiling Reveals Distinct and Mutual Effects of Diet and Inflammation in Shaping Systemic Metabolism in <i>Ldlr<sup>−/−</sup></i> Mice |
| title_full | Metabolomic Profiling Reveals Distinct and Mutual Effects of Diet and Inflammation in Shaping Systemic Metabolism in <i>Ldlr<sup>−/−</sup></i> Mice |
| title_fullStr | Metabolomic Profiling Reveals Distinct and Mutual Effects of Diet and Inflammation in Shaping Systemic Metabolism in <i>Ldlr<sup>−/−</sup></i> Mice |
| title_full_unstemmed | Metabolomic Profiling Reveals Distinct and Mutual Effects of Diet and Inflammation in Shaping Systemic Metabolism in <i>Ldlr<sup>−/−</sup></i> Mice |
| title_short | Metabolomic Profiling Reveals Distinct and Mutual Effects of Diet and Inflammation in Shaping Systemic Metabolism in <i>Ldlr<sup>−/−</sup></i> Mice |
| title_sort | metabolomic profiling reveals distinct and mutual effects of diet and inflammation in shaping systemic metabolism in i ldlr sup sup i mice |
| topic | western-type diets lipopolysaccharide (LPS) systemic metabolism systemic inflammation metabolomic profiling long-term metabolic rewiring |
| url | https://www.mdpi.com/2218-1989/10/9/336 |
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