Synthesis and Characterization of Magnetic Molecularly Imprinted Polymer for the Enrichment of Ofloxacin Enantiomers in Fish Samples
A new method for the isolation and enrichment of ofloxacin enantiomers from fish samples was developed using magnetic molecularly imprinted polymers (MMIPs). These polymers can be easily collected and rapidly separated using an external magnetic field, and also exhibit a high specific recognition fo...
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| Format: | Article |
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MDPI AG
2016-07-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/21/7/915 |
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| author | Yan-Fei Wang Huo-Xi Jin Yang-Guang Wang Li-Ye Yang Xiao-Kun OuYang Wei-Jian Wu |
| author_facet | Yan-Fei Wang Huo-Xi Jin Yang-Guang Wang Li-Ye Yang Xiao-Kun OuYang Wei-Jian Wu |
| author_sort | Yan-Fei Wang |
| collection | DOAJ |
| description | A new method for the isolation and enrichment of ofloxacin enantiomers from fish samples was developed using magnetic molecularly imprinted polymers (MMIPs). These polymers can be easily collected and rapidly separated using an external magnetic field, and also exhibit a high specific recognition for ofloxacin enantiomers. The preparation of amino-functionalized MMIPs was carried out via suspension polymerization and a ring-opening reaction using rac-ofloxacin as a template, ethylenediamine as an active group, glycidyl methacrylate and methyl methacrylate as functional monomers, divinylbenzene as a cross-linker, and Fe3O4 nanoparticles as magnetic cores. The characteristics of the MMIPs were assessed using transmission electron microscopy (TEM), X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), and vibrating sample magnetometer (VSM) measurements. Furthermore, the adsorption properties were determined using Langmuir and Freundlich isotherm models. The conditions for use of these MMIPs as magnetic solid-phase extraction (MSPE) sorbents, including pH, adsorption time, desorption time, and eluent, were investigated in detail. An extraction method using MMIPs coupled with high performance liquid chromatography (HPLC) was developed for the determination of ofloxacin enantiomers in fish samples. The limits of quantitation (LOQ) for the developed method were 0.059 and 0.067 μg∙mL−1 for levofloxacin and dextrofloxacin, respectively. The recovery of ofloxacin enantiomers ranged from 79.2% ± 5.6% to 84.4% ± 4.6% and ofloxacin enantiomers had good linear relationships within the concentration range of 0.25–5.0 μg∙mL−1 (R2 > 0.999). The obtained results demonstrate that MSPE-HPLC is a promising approach for preconcentration, purification, and simultaneous separation of ofloxacin enantiomers in biomatrix samples. |
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| series | Molecules |
| spelling | doaj.art-f7fc42b1af00407b98d997ed8aba11e52022-12-21T18:50:45ZengMDPI AGMolecules1420-30492016-07-0121791510.3390/molecules21070915molecules21070915Synthesis and Characterization of Magnetic Molecularly Imprinted Polymer for the Enrichment of Ofloxacin Enantiomers in Fish SamplesYan-Fei Wang0Huo-Xi Jin1Yang-Guang Wang2Li-Ye Yang3Xiao-Kun OuYang4Wei-Jian Wu5School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, ChinaSchool of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, ChinaSchool of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, ChinaSchool of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, ChinaSchool of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, ChinaSchool of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, ChinaA new method for the isolation and enrichment of ofloxacin enantiomers from fish samples was developed using magnetic molecularly imprinted polymers (MMIPs). These polymers can be easily collected and rapidly separated using an external magnetic field, and also exhibit a high specific recognition for ofloxacin enantiomers. The preparation of amino-functionalized MMIPs was carried out via suspension polymerization and a ring-opening reaction using rac-ofloxacin as a template, ethylenediamine as an active group, glycidyl methacrylate and methyl methacrylate as functional monomers, divinylbenzene as a cross-linker, and Fe3O4 nanoparticles as magnetic cores. The characteristics of the MMIPs were assessed using transmission electron microscopy (TEM), X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), and vibrating sample magnetometer (VSM) measurements. Furthermore, the adsorption properties were determined using Langmuir and Freundlich isotherm models. The conditions for use of these MMIPs as magnetic solid-phase extraction (MSPE) sorbents, including pH, adsorption time, desorption time, and eluent, were investigated in detail. An extraction method using MMIPs coupled with high performance liquid chromatography (HPLC) was developed for the determination of ofloxacin enantiomers in fish samples. The limits of quantitation (LOQ) for the developed method were 0.059 and 0.067 μg∙mL−1 for levofloxacin and dextrofloxacin, respectively. The recovery of ofloxacin enantiomers ranged from 79.2% ± 5.6% to 84.4% ± 4.6% and ofloxacin enantiomers had good linear relationships within the concentration range of 0.25–5.0 μg∙mL−1 (R2 > 0.999). The obtained results demonstrate that MSPE-HPLC is a promising approach for preconcentration, purification, and simultaneous separation of ofloxacin enantiomers in biomatrix samples.http://www.mdpi.com/1420-3049/21/7/915magnetic solid-phase extractionofloxacin enantiomersmagnetic molecularly imprinted polymerschiral HPLC |
| spellingShingle | Yan-Fei Wang Huo-Xi Jin Yang-Guang Wang Li-Ye Yang Xiao-Kun OuYang Wei-Jian Wu Synthesis and Characterization of Magnetic Molecularly Imprinted Polymer for the Enrichment of Ofloxacin Enantiomers in Fish Samples Molecules magnetic solid-phase extraction ofloxacin enantiomers magnetic molecularly imprinted polymers chiral HPLC |
| title | Synthesis and Characterization of Magnetic Molecularly Imprinted Polymer for the Enrichment of Ofloxacin Enantiomers in Fish Samples |
| title_full | Synthesis and Characterization of Magnetic Molecularly Imprinted Polymer for the Enrichment of Ofloxacin Enantiomers in Fish Samples |
| title_fullStr | Synthesis and Characterization of Magnetic Molecularly Imprinted Polymer for the Enrichment of Ofloxacin Enantiomers in Fish Samples |
| title_full_unstemmed | Synthesis and Characterization of Magnetic Molecularly Imprinted Polymer for the Enrichment of Ofloxacin Enantiomers in Fish Samples |
| title_short | Synthesis and Characterization of Magnetic Molecularly Imprinted Polymer for the Enrichment of Ofloxacin Enantiomers in Fish Samples |
| title_sort | synthesis and characterization of magnetic molecularly imprinted polymer for the enrichment of ofloxacin enantiomers in fish samples |
| topic | magnetic solid-phase extraction ofloxacin enantiomers magnetic molecularly imprinted polymers chiral HPLC |
| url | http://www.mdpi.com/1420-3049/21/7/915 |
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