Lactoferrin Ameliorates Ovalbumin-Induced Asthma in Mice through Reducing Dendritic-Cell-Derived Th2 Cell Responses
Asthma is a chronic respiratory disease with symptoms such as expiratory airflow narrowing and airway hyperresponsiveness (AHR). Millions of people suffer from asthma and are at risk of life-threatening conditions. Lactoferrin (LF) is a glycoprotein with multiple physiological functions, including a...
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/22/14185 |
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| author | Chi-Chien Lin Kai-Cheng Chuang Shih-Wei Chen Ya-Hsuan Chao Chih-Ching Yen Shang-Hsun Yang Wei Chen Kuang-Hsi Chang Yu-Kang Chang Chuan-Mu Chen |
| author_facet | Chi-Chien Lin Kai-Cheng Chuang Shih-Wei Chen Ya-Hsuan Chao Chih-Ching Yen Shang-Hsun Yang Wei Chen Kuang-Hsi Chang Yu-Kang Chang Chuan-Mu Chen |
| author_sort | Chi-Chien Lin |
| collection | DOAJ |
| description | Asthma is a chronic respiratory disease with symptoms such as expiratory airflow narrowing and airway hyperresponsiveness (AHR). Millions of people suffer from asthma and are at risk of life-threatening conditions. Lactoferrin (LF) is a glycoprotein with multiple physiological functions, including antioxidant, anti-inflammatory, antimicrobial, and antitumoral activities. LF has been shown to function in immunoregulatory activities in ovalbumin (OVA)-induced delayed type hypersensitivity (DTH) in mice. Hence, the purpose of this study was to investigate the roles of LF in AHR and the functions of dendritic cells (DCs) and Th2-related responses in asthma. Twenty 8-week-old male BALB/c mice were divided into normal control (NC), ovalbumin (OVA)-sensitized, and OVA-sensitized with low dose of LF (100 mg/kg) or high dose of LF (300 mg/kg) treatment groups. The mice were challenged by intranasal instillation with 5% OVA on the 21st to 27th day after the start of the sensitization period. The AHR, cytokines in bronchoalveolar lavage fluid, and pulmonary histology of each mouse were measured. Serum OVA-specific IgE and IgG1 and OVA-specific splenocyte responses were further detected. The results showed that LF exhibited protective effects in ameliorating AHR, as well as lung inflammation and damage, in reducing the expression of Th2 cytokines and the secretion of allergen-specific antibodies, in influencing the functions of DCs, and in decreasing the level of Th2 immune responses in a BALB/c mouse model of OVA-induced allergic asthma. Importantly, we demonstrated that LF has practical application in reducing DC-induced Th2 cell responses in asthma. In conclusion, LF exhibits anti-inflammation and immunoregulation activities in OVA-induced allergic asthma. These results suggest that LF may act as a supplement to prevent asthma-induced lung injury and provide an additional agent for reducing asthma severity. |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T18:17:14Z |
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| spelling | doaj.art-f7fce137ae614e15b0543e9ca6d56cfc2023-11-24T08:39:59ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123221418510.3390/ijms232214185Lactoferrin Ameliorates Ovalbumin-Induced Asthma in Mice through Reducing Dendritic-Cell-Derived Th2 Cell ResponsesChi-Chien Lin0Kai-Cheng Chuang1Shih-Wei Chen2Ya-Hsuan Chao3Chih-Ching Yen4Shang-Hsun Yang5Wei Chen6Kuang-Hsi Chang7Yu-Kang Chang8Chuan-Mu Chen9Department of Life Sciences, Ph.D. Program in Translational Medicine, National Chung Hsing University, Taichung 402, TaiwanDepartment of Life Sciences, Ph.D. Program in Translational Medicine, National Chung Hsing University, Taichung 402, TaiwanDepartment of Life Sciences, Ph.D. Program in Translational Medicine, National Chung Hsing University, Taichung 402, TaiwanInstitute of Biomedical Sciences, National Chung Hsing University, Taichung 402, TaiwanDepartment of Life Sciences, Ph.D. Program in Translational Medicine, National Chung Hsing University, Taichung 402, TaiwanDepartment of Physiology, Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan 701, TaiwanDivision of Pulmonary and Critical Care Medicine, Chia-Yi Christian Hospital, Chiayi 600, TaiwanDepartment of Medical Research, Tungs’ Taichung MetroHarbor Hospital, Taichung 435, TaiwanDepartment of Medical Research, Tungs’ Taichung MetroHarbor Hospital, Taichung 435, TaiwanDepartment of Life Sciences, Ph.D. Program in Translational Medicine, National Chung Hsing University, Taichung 402, TaiwanAsthma is a chronic respiratory disease with symptoms such as expiratory airflow narrowing and airway hyperresponsiveness (AHR). Millions of people suffer from asthma and are at risk of life-threatening conditions. Lactoferrin (LF) is a glycoprotein with multiple physiological functions, including antioxidant, anti-inflammatory, antimicrobial, and antitumoral activities. LF has been shown to function in immunoregulatory activities in ovalbumin (OVA)-induced delayed type hypersensitivity (DTH) in mice. Hence, the purpose of this study was to investigate the roles of LF in AHR and the functions of dendritic cells (DCs) and Th2-related responses in asthma. Twenty 8-week-old male BALB/c mice were divided into normal control (NC), ovalbumin (OVA)-sensitized, and OVA-sensitized with low dose of LF (100 mg/kg) or high dose of LF (300 mg/kg) treatment groups. The mice were challenged by intranasal instillation with 5% OVA on the 21st to 27th day after the start of the sensitization period. The AHR, cytokines in bronchoalveolar lavage fluid, and pulmonary histology of each mouse were measured. Serum OVA-specific IgE and IgG1 and OVA-specific splenocyte responses were further detected. The results showed that LF exhibited protective effects in ameliorating AHR, as well as lung inflammation and damage, in reducing the expression of Th2 cytokines and the secretion of allergen-specific antibodies, in influencing the functions of DCs, and in decreasing the level of Th2 immune responses in a BALB/c mouse model of OVA-induced allergic asthma. Importantly, we demonstrated that LF has practical application in reducing DC-induced Th2 cell responses in asthma. In conclusion, LF exhibits anti-inflammation and immunoregulation activities in OVA-induced allergic asthma. These results suggest that LF may act as a supplement to prevent asthma-induced lung injury and provide an additional agent for reducing asthma severity.https://www.mdpi.com/1422-0067/23/22/14185lactoferrinairway hyperresponsivenesspulmonary inflammationallergyasthma |
| spellingShingle | Chi-Chien Lin Kai-Cheng Chuang Shih-Wei Chen Ya-Hsuan Chao Chih-Ching Yen Shang-Hsun Yang Wei Chen Kuang-Hsi Chang Yu-Kang Chang Chuan-Mu Chen Lactoferrin Ameliorates Ovalbumin-Induced Asthma in Mice through Reducing Dendritic-Cell-Derived Th2 Cell Responses International Journal of Molecular Sciences lactoferrin airway hyperresponsiveness pulmonary inflammation allergy asthma |
| title | Lactoferrin Ameliorates Ovalbumin-Induced Asthma in Mice through Reducing Dendritic-Cell-Derived Th2 Cell Responses |
| title_full | Lactoferrin Ameliorates Ovalbumin-Induced Asthma in Mice through Reducing Dendritic-Cell-Derived Th2 Cell Responses |
| title_fullStr | Lactoferrin Ameliorates Ovalbumin-Induced Asthma in Mice through Reducing Dendritic-Cell-Derived Th2 Cell Responses |
| title_full_unstemmed | Lactoferrin Ameliorates Ovalbumin-Induced Asthma in Mice through Reducing Dendritic-Cell-Derived Th2 Cell Responses |
| title_short | Lactoferrin Ameliorates Ovalbumin-Induced Asthma in Mice through Reducing Dendritic-Cell-Derived Th2 Cell Responses |
| title_sort | lactoferrin ameliorates ovalbumin induced asthma in mice through reducing dendritic cell derived th2 cell responses |
| topic | lactoferrin airway hyperresponsiveness pulmonary inflammation allergy asthma |
| url | https://www.mdpi.com/1422-0067/23/22/14185 |
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