In vitro assembly of plasmid DNA for direct cloning in Lactiplantibacillus plantarum WCSF1.

Lactobacilli are gram-positive bacteria that are growing in importance for the healthcare industry and genetically engineering them as living therapeutics is highly sought after. However, progress in this field is hindered since most strains are difficult to genetically manipulate, partly due to the...

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Bibliographic Details
Main Authors: Marc Blanch-Asensio, Sourik Dey, Shrikrishnan Sankaran
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2023-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0281625