Effects of hypercaloric diet-induced hyperinsulinemia and hyperlipidemia on the ovarian follicular development in mice
Long-term hypercaloric diets may adversely affect the development of ovarian follicles. We investigated the effects of high sugar (HS), high fat low sugar (HFLS), and high fat normal sugar (HFNS) diets on the ovarian follicle development in mice fed with these diets as compared to those fed with nor...
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| Format: | Article |
| Language: | English |
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The Society for Reproduction and Development
2022-03-01
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| Series: | The Journal of Reproduction and Development |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jrd/68/3/68_2021-132/_pdf/-char/en |
| _version_ | 1827767665931845632 |
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| author | Qiao-Li ZHANG Yan WANG Jian-Sheng LIU Yan-Zhi DU |
| author_facet | Qiao-Li ZHANG Yan WANG Jian-Sheng LIU Yan-Zhi DU |
| author_sort | Qiao-Li ZHANG |
| collection | DOAJ |
| description | Long-term hypercaloric diets may adversely affect the development of ovarian follicles. We investigated the effects of high sugar (HS), high fat low sugar (HFLS), and high fat normal sugar (HFNS) diets on the ovarian follicle development in mice fed with these diets as compared to those fed with normal diet (control) for 180 days. Body weight, gonadal fat, glucose, lipid, insulin, estrous cycle, sex hormones and ovarian tissues were examined, and metabolism-related protein expression in the ovaries was evaluated by immunoblotting. The mice fed with hypercaloric diets showed hyperinsulinemia and hyperlipidemia, and exhibited heavier body and gonadal fat weights, longer estrous cycles, and fewer preantral and antral follicles than mice fed with normal diet. The sex hormone levels in the blood were similar to those in controls, except for significantly elevated estradiol levels in the HS diet group. The AMPKα phosphorylation was reduced, while AKT phosphorylation and caspase-3 levels were increased in the ovarian tissues of mice in all three hypercaloric diet groups than those in control. Taken together, the results suggest hyperinsulinemia and hyperlipidemia as possible mechanisms that impair the development of ovarian follicles in response to long-term exposure to unhealthy hypercaloric diets. |
| first_indexed | 2024-03-11T12:02:13Z |
| format | Article |
| id | doaj.art-f8087899d5dd4329997e877d3869235a |
| institution | Directory Open Access Journal |
| issn | 0916-8818 1348-4400 |
| language | English |
| last_indexed | 2024-03-11T12:02:13Z |
| publishDate | 2022-03-01 |
| publisher | The Society for Reproduction and Development |
| record_format | Article |
| series | The Journal of Reproduction and Development |
| spelling | doaj.art-f8087899d5dd4329997e877d3869235a2023-11-08T05:06:30ZengThe Society for Reproduction and DevelopmentThe Journal of Reproduction and Development0916-88181348-44002022-03-0168317318010.1262/jrd.2021-132jrdEffects of hypercaloric diet-induced hyperinsulinemia and hyperlipidemia on the ovarian follicular development in miceQiao-Li ZHANG0Yan WANG1Jian-Sheng LIU2Yan-Zhi DU3Department of Human Reproductive Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing 100026, ChinaDepartment of Immunology and Pathogenic Biology, Molecular Bacteriology Laboratory, Key Laboratory of Environment and Genes Related to Diseases of Chinese Ministry of Education, School of Medicine, Xi’an Jiaotong University, Xi’an 710061, ChinaShanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Center for Reproductive Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200135, ChinaShanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Center for Reproductive Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200135, ChinaLong-term hypercaloric diets may adversely affect the development of ovarian follicles. We investigated the effects of high sugar (HS), high fat low sugar (HFLS), and high fat normal sugar (HFNS) diets on the ovarian follicle development in mice fed with these diets as compared to those fed with normal diet (control) for 180 days. Body weight, gonadal fat, glucose, lipid, insulin, estrous cycle, sex hormones and ovarian tissues were examined, and metabolism-related protein expression in the ovaries was evaluated by immunoblotting. The mice fed with hypercaloric diets showed hyperinsulinemia and hyperlipidemia, and exhibited heavier body and gonadal fat weights, longer estrous cycles, and fewer preantral and antral follicles than mice fed with normal diet. The sex hormone levels in the blood were similar to those in controls, except for significantly elevated estradiol levels in the HS diet group. The AMPKα phosphorylation was reduced, while AKT phosphorylation and caspase-3 levels were increased in the ovarian tissues of mice in all three hypercaloric diet groups than those in control. Taken together, the results suggest hyperinsulinemia and hyperlipidemia as possible mechanisms that impair the development of ovarian follicles in response to long-term exposure to unhealthy hypercaloric diets.https://www.jstage.jst.go.jp/article/jrd/68/3/68_2021-132/_pdf/-char/enfollicular developmenthypercaloric dietshyperinsulinemiahyperlipidemia |
| spellingShingle | Qiao-Li ZHANG Yan WANG Jian-Sheng LIU Yan-Zhi DU Effects of hypercaloric diet-induced hyperinsulinemia and hyperlipidemia on the ovarian follicular development in mice The Journal of Reproduction and Development follicular development hypercaloric diets hyperinsulinemia hyperlipidemia |
| title | Effects of hypercaloric diet-induced hyperinsulinemia and hyperlipidemia on the ovarian follicular development in mice |
| title_full | Effects of hypercaloric diet-induced hyperinsulinemia and hyperlipidemia on the ovarian follicular development in mice |
| title_fullStr | Effects of hypercaloric diet-induced hyperinsulinemia and hyperlipidemia on the ovarian follicular development in mice |
| title_full_unstemmed | Effects of hypercaloric diet-induced hyperinsulinemia and hyperlipidemia on the ovarian follicular development in mice |
| title_short | Effects of hypercaloric diet-induced hyperinsulinemia and hyperlipidemia on the ovarian follicular development in mice |
| title_sort | effects of hypercaloric diet induced hyperinsulinemia and hyperlipidemia on the ovarian follicular development in mice |
| topic | follicular development hypercaloric diets hyperinsulinemia hyperlipidemia |
| url | https://www.jstage.jst.go.jp/article/jrd/68/3/68_2021-132/_pdf/-char/en |
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