Studying DNA Double-Strand Break Repair: An Ever-Growing Toolbox
To ward off against the catastrophic consequences of persistent DNA double-strand breaks (DSBs), eukaryotic cells have developed a set of complex signaling networks that detect these DNA lesions, orchestrate cell cycle checkpoints and ultimately lead to their repair. Collectively, these signaling ne...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-02-01
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| Series: | Frontiers in Molecular Biosciences |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmolb.2020.00024/full |
| _version_ | 1819110853389058048 |
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| author | Alexandra C. Vítor Pablo Huertas Pablo Huertas Gaëlle Legube Sérgio F. de Almeida |
| author_facet | Alexandra C. Vítor Pablo Huertas Pablo Huertas Gaëlle Legube Sérgio F. de Almeida |
| author_sort | Alexandra C. Vítor |
| collection | DOAJ |
| description | To ward off against the catastrophic consequences of persistent DNA double-strand breaks (DSBs), eukaryotic cells have developed a set of complex signaling networks that detect these DNA lesions, orchestrate cell cycle checkpoints and ultimately lead to their repair. Collectively, these signaling networks comprise the DNA damage response (DDR). The current knowledge of the molecular determinants and mechanistic details of the DDR owes greatly to the continuous development of ground-breaking experimental tools that couple the controlled induction of DSBs at distinct genomic positions with assays and reporters to investigate DNA repair pathways, their impact on other DNA-templated processes and the specific contribution of the chromatin environment. In this review, we present these tools, discuss their pros and cons and illustrate their contribution to our current understanding of the DDR. |
| first_indexed | 2024-12-22T03:48:19Z |
| format | Article |
| id | doaj.art-f80b5fb8431a48af955ae03d4fdc956d |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-12-22T03:48:19Z |
| publishDate | 2020-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-f80b5fb8431a48af955ae03d4fdc956d2022-12-21T18:40:05ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2020-02-01710.3389/fmolb.2020.00024512455Studying DNA Double-Strand Break Repair: An Ever-Growing ToolboxAlexandra C. Vítor0Pablo Huertas1Pablo Huertas2Gaëlle Legube3Sérgio F. de Almeida4Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa, Lisbon, PortugalDepartment of Genetics, University of Seville, Seville, SpainCentro Andaluz de Biología Molecular y Medicina Regenerativa-CABIMER, Universidad de Sevilla-CSIC-Universidad Pablo de Olavide, Seville, SpainLBCMCP, Centre de Biologie Integrative (CBI), CNRS, Université de Toulouse, Toulouse, FranceInstituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa, Lisbon, PortugalTo ward off against the catastrophic consequences of persistent DNA double-strand breaks (DSBs), eukaryotic cells have developed a set of complex signaling networks that detect these DNA lesions, orchestrate cell cycle checkpoints and ultimately lead to their repair. Collectively, these signaling networks comprise the DNA damage response (DDR). The current knowledge of the molecular determinants and mechanistic details of the DDR owes greatly to the continuous development of ground-breaking experimental tools that couple the controlled induction of DSBs at distinct genomic positions with assays and reporters to investigate DNA repair pathways, their impact on other DNA-templated processes and the specific contribution of the chromatin environment. In this review, we present these tools, discuss their pros and cons and illustrate their contribution to our current understanding of the DDR.https://www.frontiersin.org/article/10.3389/fmolb.2020.00024/fullDNA repairhomologous recombination (HR)non-homologous DNA end joiningchromatinDNA damage |
| spellingShingle | Alexandra C. Vítor Pablo Huertas Pablo Huertas Gaëlle Legube Sérgio F. de Almeida Studying DNA Double-Strand Break Repair: An Ever-Growing Toolbox Frontiers in Molecular Biosciences DNA repair homologous recombination (HR) non-homologous DNA end joining chromatin DNA damage |
| title | Studying DNA Double-Strand Break Repair: An Ever-Growing Toolbox |
| title_full | Studying DNA Double-Strand Break Repair: An Ever-Growing Toolbox |
| title_fullStr | Studying DNA Double-Strand Break Repair: An Ever-Growing Toolbox |
| title_full_unstemmed | Studying DNA Double-Strand Break Repair: An Ever-Growing Toolbox |
| title_short | Studying DNA Double-Strand Break Repair: An Ever-Growing Toolbox |
| title_sort | studying dna double strand break repair an ever growing toolbox |
| topic | DNA repair homologous recombination (HR) non-homologous DNA end joining chromatin DNA damage |
| url | https://www.frontiersin.org/article/10.3389/fmolb.2020.00024/full |
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