Tumor-Infiltrating CD45RO<sup>+</sup> Memory Cells Are Associated with Favorable Prognosis in Oral Squamous Cell Carcinoma Patients
Background: Tumor-infiltrating lymphocytes (TILs) have been used to predict the prognosis of solid tumors. In this study, we investigated which molecules in TILs play a role in the prognosis of patients with oral squamous cell carcinoma (OSCC). Methods: In a retrospective case-control study, we immu...
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MDPI AG
2023-04-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/15/8/2221 |
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| author | Nanako Ito Sachiko Yamasaki Tomoaki Shintani Kensaku Matsui Fumitaka Obayashi Koichi Koizumi Ryouji Tani Souichi Yanamoto Tetsuji Okamoto |
| author_facet | Nanako Ito Sachiko Yamasaki Tomoaki Shintani Kensaku Matsui Fumitaka Obayashi Koichi Koizumi Ryouji Tani Souichi Yanamoto Tetsuji Okamoto |
| author_sort | Nanako Ito |
| collection | DOAJ |
| description | Background: Tumor-infiltrating lymphocytes (TILs) have been used to predict the prognosis of solid tumors. In this study, we investigated which molecules in TILs play a role in the prognosis of patients with oral squamous cell carcinoma (OSCC). Methods: In a retrospective case-control study, we immunohistochemically evaluated the expression of CD3, CD8, CD45RO, Granzyme B, and the major histocompatibility complex class I chain-related molecule A (MICA) of the histocompatibility complex as predictors of prognosis in 33 patients with OSCC. The patients were classified as TILs<sup>High</sup> or TILs<sup>Low</sup> according to the number of TILs for each molecule in the central tumor (CT) and invasive margin (IM). Furthermore, MICA expression scores were determined based on the intensity of the staining. Results: CD45RO<sup>+</sup>/TIL in the nonrecurrent group were significantly higher than those in the recurrent group in the CT and IM areas (<i>p</i> < 0.05). The disease-free survival/overall survival rate of the CD45RO<sup>+</sup>/TILs<sup>Low</sup> group in the CT and IM areas and the Granzyme B<sup>+</sup>/TILs<sup>Low</sup> group in the IM area was significantly lower than that of the CD45RO<sup>+</sup>/TILs<sup>High</sup> group and the Granzyme B<sup>+</sup>/TILs<sup>High</sup> group, respectively (<i>p</i> < 0.05). Furthermore, the MICA expression score of tumors around the CD45RO<sup>+</sup>/TILs<sup>High</sup> group was significantly higher than that of the CD45RO<sup>+</sup>/TILs<sup>Low</sup> group (<i>p</i> < 0.05). Conclusions: A high ratio of CD45RO-expressing TILs was associated with a disease-free/overall survival improvement in OSCC patients. Furthermore, the number of TILs that express CD45RO was associated with the expression of MICA in tumors. These results suggest that CD45RO-expressing TILs are useful biomarkers for OSCC. |
| first_indexed | 2024-03-11T05:10:38Z |
| format | Article |
| id | doaj.art-f80d6b65704446c2849cd4ae4c2ef247 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-11T05:10:38Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-f80d6b65704446c2849cd4ae4c2ef2472023-11-17T18:37:58ZengMDPI AGCancers2072-66942023-04-01158222110.3390/cancers15082221Tumor-Infiltrating CD45RO<sup>+</sup> Memory Cells Are Associated with Favorable Prognosis in Oral Squamous Cell Carcinoma PatientsNanako Ito0Sachiko Yamasaki1Tomoaki Shintani2Kensaku Matsui3Fumitaka Obayashi4Koichi Koizumi5Ryouji Tani6Souichi Yanamoto7Tetsuji Okamoto8Department of Oral Oncology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, JapanDepartment of Oral Oncology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, JapanCenter of Oral Clinical Examination, Hiroshima University Hospital, Hiroshima 734-8551, JapanDepartment of Oral Oncology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, JapanDepartment of Oral Oncology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, JapanDepartment of Oral Oncology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, JapanDepartment of Oral Oncology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, JapanDepartment of Oral Oncology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, JapanDepartment of Oral Oncology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, JapanBackground: Tumor-infiltrating lymphocytes (TILs) have been used to predict the prognosis of solid tumors. In this study, we investigated which molecules in TILs play a role in the prognosis of patients with oral squamous cell carcinoma (OSCC). Methods: In a retrospective case-control study, we immunohistochemically evaluated the expression of CD3, CD8, CD45RO, Granzyme B, and the major histocompatibility complex class I chain-related molecule A (MICA) of the histocompatibility complex as predictors of prognosis in 33 patients with OSCC. The patients were classified as TILs<sup>High</sup> or TILs<sup>Low</sup> according to the number of TILs for each molecule in the central tumor (CT) and invasive margin (IM). Furthermore, MICA expression scores were determined based on the intensity of the staining. Results: CD45RO<sup>+</sup>/TIL in the nonrecurrent group were significantly higher than those in the recurrent group in the CT and IM areas (<i>p</i> < 0.05). The disease-free survival/overall survival rate of the CD45RO<sup>+</sup>/TILs<sup>Low</sup> group in the CT and IM areas and the Granzyme B<sup>+</sup>/TILs<sup>Low</sup> group in the IM area was significantly lower than that of the CD45RO<sup>+</sup>/TILs<sup>High</sup> group and the Granzyme B<sup>+</sup>/TILs<sup>High</sup> group, respectively (<i>p</i> < 0.05). Furthermore, the MICA expression score of tumors around the CD45RO<sup>+</sup>/TILs<sup>High</sup> group was significantly higher than that of the CD45RO<sup>+</sup>/TILs<sup>Low</sup> group (<i>p</i> < 0.05). Conclusions: A high ratio of CD45RO-expressing TILs was associated with a disease-free/overall survival improvement in OSCC patients. Furthermore, the number of TILs that express CD45RO was associated with the expression of MICA in tumors. These results suggest that CD45RO-expressing TILs are useful biomarkers for OSCC.https://www.mdpi.com/2072-6694/15/8/2221MHC class I chain-related molecule ACD45ROtumor-infiltrating lymphocyteoral squamous cell carcinoma |
| spellingShingle | Nanako Ito Sachiko Yamasaki Tomoaki Shintani Kensaku Matsui Fumitaka Obayashi Koichi Koizumi Ryouji Tani Souichi Yanamoto Tetsuji Okamoto Tumor-Infiltrating CD45RO<sup>+</sup> Memory Cells Are Associated with Favorable Prognosis in Oral Squamous Cell Carcinoma Patients Cancers MHC class I chain-related molecule A CD45RO tumor-infiltrating lymphocyte oral squamous cell carcinoma |
| title | Tumor-Infiltrating CD45RO<sup>+</sup> Memory Cells Are Associated with Favorable Prognosis in Oral Squamous Cell Carcinoma Patients |
| title_full | Tumor-Infiltrating CD45RO<sup>+</sup> Memory Cells Are Associated with Favorable Prognosis in Oral Squamous Cell Carcinoma Patients |
| title_fullStr | Tumor-Infiltrating CD45RO<sup>+</sup> Memory Cells Are Associated with Favorable Prognosis in Oral Squamous Cell Carcinoma Patients |
| title_full_unstemmed | Tumor-Infiltrating CD45RO<sup>+</sup> Memory Cells Are Associated with Favorable Prognosis in Oral Squamous Cell Carcinoma Patients |
| title_short | Tumor-Infiltrating CD45RO<sup>+</sup> Memory Cells Are Associated with Favorable Prognosis in Oral Squamous Cell Carcinoma Patients |
| title_sort | tumor infiltrating cd45ro sup sup memory cells are associated with favorable prognosis in oral squamous cell carcinoma patients |
| topic | MHC class I chain-related molecule A CD45RO tumor-infiltrating lymphocyte oral squamous cell carcinoma |
| url | https://www.mdpi.com/2072-6694/15/8/2221 |
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