Single strand conformation polymorphism (SSCP) detection in six genes in Portuguese indigenous sheep breed
Evaluation of the genetic diversity for six genes in forty animals of the Portuguese indigenous sheep breed (Ovis aries) ""Churra da Terra Quente"" was done. A non-radioactive method to allow single-strand conformation polymorphism (SSCP) detection was optimised, starting from ge...
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| Format: | Article |
| Language: | English |
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Presses Agronomiques de Gembloux
2001-01-01
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| Series: | Biotechnologie, Agronomie, Société et Environnement |
| Subjects: | |
| Online Access: | http://www.pressesagro.be/base/text/v5n1/7.pdf |
| _version_ | 1811277539749920768 |
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| author | Guedes-Pinto H. Bastos E. Cravador A. Azevedo J. |
| author_facet | Guedes-Pinto H. Bastos E. Cravador A. Azevedo J. |
| author_sort | Guedes-Pinto H. |
| collection | DOAJ |
| description | Evaluation of the genetic diversity for six genes in forty animals of the Portuguese indigenous sheep breed (Ovis aries) ""Churra da Terra Quente"" was done. A non-radioactive method to allow single-strand conformation polymorphism (SSCP) detection was optimised, starting from genomic DNA and PCR amplification of seven fragments: exon 1 of the alpha-lactalbumin gene; exons 10 and 11 of the alpha s1-casein gene; exon 7 of the beta-casein gene; exon 4 of the kappa-casein gene; exons 4 and 5 of the growth hormone gene and exon 6 of the growth hormone receptor gene. Polymorphisms were detected in five of the seven PCR products. Only kappa-casein and growth hormone receptor were monomorphic. Alpha-lactalbumin and alpha s1-casein exons showed three conformational patterns, beta-casein and growth hormone exon 4 showed two electrophoretic patterns and growth hormone exon 5 showed five conformational patterns. These data provide evidence that ""Churra da Terra Quente"" has a high genetic variability, which opens interesting prospects for future selection programs and also for preservation strategies. Also, our data show that PCR-SSCP is an appropriate tool for evaluating genetic variability. |
| first_indexed | 2024-04-13T00:17:29Z |
| format | Article |
| id | doaj.art-f80dcde47d5948f3b0491fd6b9475e65 |
| institution | Directory Open Access Journal |
| issn | 1370-6233 1780-4507 |
| language | English |
| last_indexed | 2024-04-13T00:17:29Z |
| publishDate | 2001-01-01 |
| publisher | Presses Agronomiques de Gembloux |
| record_format | Article |
| series | Biotechnologie, Agronomie, Société et Environnement |
| spelling | doaj.art-f80dcde47d5948f3b0491fd6b9475e652022-12-22T03:10:53ZengPresses Agronomiques de GemblouxBiotechnologie, Agronomie, Société et Environnement1370-62331780-45072001-01-0151715Single strand conformation polymorphism (SSCP) detection in six genes in Portuguese indigenous sheep breedGuedes-Pinto H.Bastos E.Cravador A.Azevedo J.Evaluation of the genetic diversity for six genes in forty animals of the Portuguese indigenous sheep breed (Ovis aries) ""Churra da Terra Quente"" was done. A non-radioactive method to allow single-strand conformation polymorphism (SSCP) detection was optimised, starting from genomic DNA and PCR amplification of seven fragments: exon 1 of the alpha-lactalbumin gene; exons 10 and 11 of the alpha s1-casein gene; exon 7 of the beta-casein gene; exon 4 of the kappa-casein gene; exons 4 and 5 of the growth hormone gene and exon 6 of the growth hormone receptor gene. Polymorphisms were detected in five of the seven PCR products. Only kappa-casein and growth hormone receptor were monomorphic. Alpha-lactalbumin and alpha s1-casein exons showed three conformational patterns, beta-casein and growth hormone exon 4 showed two electrophoretic patterns and growth hormone exon 5 showed five conformational patterns. These data provide evidence that ""Churra da Terra Quente"" has a high genetic variability, which opens interesting prospects for future selection programs and also for preservation strategies. Also, our data show that PCR-SSCP is an appropriate tool for evaluating genetic variability.http://www.pressesagro.be/base/text/v5n1/7.pdfsheepland-racespolymorphismgenetic-variationsomatotropinhormone-receptorsgenesdnapcrgenetic-variationanimal-breedingportugalacidsbovidaebreeds-animalscaprinaecell-structurechromosomesdomestic-animalseuropehormoneslivestockmammalsnucleic-acidsnucleic-compoundsnucleusorganic-acidspituitary-hormonesproteinsruminantssouthern-europetaxauseful-animalswestern-europe |
| spellingShingle | Guedes-Pinto H. Bastos E. Cravador A. Azevedo J. Single strand conformation polymorphism (SSCP) detection in six genes in Portuguese indigenous sheep breed Biotechnologie, Agronomie, Société et Environnement sheep land-races polymorphism genetic-variation somatotropin hormone-receptors genes dna pcr genetic-variation animal-breeding portugal acids bovidae breeds-animals caprinae cell-structure chromosomes domestic-animals europe hormones livestock mammals nucleic-acids nucleic-compounds nucleus organic-acids pituitary-hormones proteins ruminants southern-europe taxa useful-animals western-europe |
| title | Single strand conformation polymorphism (SSCP) detection in six genes in Portuguese indigenous sheep breed |
| title_full | Single strand conformation polymorphism (SSCP) detection in six genes in Portuguese indigenous sheep breed |
| title_fullStr | Single strand conformation polymorphism (SSCP) detection in six genes in Portuguese indigenous sheep breed |
| title_full_unstemmed | Single strand conformation polymorphism (SSCP) detection in six genes in Portuguese indigenous sheep breed |
| title_short | Single strand conformation polymorphism (SSCP) detection in six genes in Portuguese indigenous sheep breed |
| title_sort | single strand conformation polymorphism sscp detection in six genes in portuguese indigenous sheep breed |
| topic | sheep land-races polymorphism genetic-variation somatotropin hormone-receptors genes dna pcr genetic-variation animal-breeding portugal acids bovidae breeds-animals caprinae cell-structure chromosomes domestic-animals europe hormones livestock mammals nucleic-acids nucleic-compounds nucleus organic-acids pituitary-hormones proteins ruminants southern-europe taxa useful-animals western-europe |
| url | http://www.pressesagro.be/base/text/v5n1/7.pdf |
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