Laboratory study of the effects of leek lectin (APA) in transgenic tobacco plants on the development of cotton leafworm Spodoptera littoralis (Lepidoptera: Noctuidae)
Transgenic tobacco plants expressing the Allium porrum L. (leek) agglutinin (called APA) under the control of the 35S constitutive promoter were tested for their insecticidal activity against the cotton leafworm, Spodoptera littoralis (Lepidoptera: Noctuidae). Southern blot and PCR analysis confirme...
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| Format: | Article |
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Institute of Entomology, Biology Centre, Czech Academy of Science
2009-03-01
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| Series: | European Journal of Entomology |
| Subjects: | |
| Online Access: | https://www.eje.cz/artkey/eje-200901-0003_Laboratory_study_of_the_effects_of_leek_lectin_APA_in_transgenic_tobacco_plants_on_the_development_of_cotton.php |
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| author | Amin SADEGHI Guy SMAGGHE Esmeralda JURADO-JÁCOME Willy J. PEUMANS Els J.M. VAN DAMME |
| author_facet | Amin SADEGHI Guy SMAGGHE Esmeralda JURADO-JÁCOME Willy J. PEUMANS Els J.M. VAN DAMME |
| author_sort | Amin SADEGHI |
| collection | DOAJ |
| description | Transgenic tobacco plants expressing the Allium porrum L. (leek) agglutinin (called APA) under the control of the 35S constitutive promoter were tested for their insecticidal activity against the cotton leafworm, Spodoptera littoralis (Lepidoptera: Noctuidae). Southern blot and PCR analysis confirmed that the APA gene was integrated into the plant genome. Northern and Western blots as well as semi-quantitative agglutination assays revealed lectin expression at various levels in the transgenic lines. Biochemical analyses indicated that the recombinant APA has the same molecular structure as the native lectin. Native and recombinant lectin have the same N-terminal amino acid sequence. Bioassays using detached leaves from transgenic tobacco plants demonstrated that the ectopically expressed APA significantly (p < 0.05) reduced the weight gain of 2nd-instar larvae of S. littoralis. This inhibitory effect was observed throughout the experiment and on day 11 the caterpillars fed on transgenic tobacco leaves were 25-30% lighter in weight than the control caterpillars fed on wild type plants. In addition the lectin retarded the development of the larvae and metamorphosis, reduced pupal weight and increased mortality rate. These findings suggest that APA is a suitable insect resistance protein for integrating into plant genomes for controlling S. littoralis. |
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| id | doaj.art-f80f50fdf0d04d0696c7c6139bc5bb47 |
| institution | Directory Open Access Journal |
| issn | 1210-5759 1802-8829 |
| language | English |
| last_indexed | 2024-12-17T23:59:29Z |
| publishDate | 2009-03-01 |
| publisher | Institute of Entomology, Biology Centre, Czech Academy of Science |
| record_format | Article |
| series | European Journal of Entomology |
| spelling | doaj.art-f80f50fdf0d04d0696c7c6139bc5bb472022-12-21T21:28:00ZengInstitute of Entomology, Biology Centre, Czech Academy of ScienceEuropean Journal of Entomology1210-57591802-88292009-03-011061212810.14411/eje.2009.003eje-200901-0003Laboratory study of the effects of leek lectin (APA) in transgenic tobacco plants on the development of cotton leafworm Spodoptera littoralis (Lepidoptera: Noctuidae)Amin SADEGHI0Guy SMAGGHE1Esmeralda JURADO-JÁCOME2Willy J. PEUMANS3Els J.M. VAN DAMME4Laboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Gent, BelgiumLaboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Gent, BelgiumLaboratory of Biochemistry and Glycobiology, Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Gent, BelgiumLaboratory of Biochemistry and Glycobiology, Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Gent, BelgiumLaboratory of Biochemistry and Glycobiology, Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Gent, BelgiumTransgenic tobacco plants expressing the Allium porrum L. (leek) agglutinin (called APA) under the control of the 35S constitutive promoter were tested for their insecticidal activity against the cotton leafworm, Spodoptera littoralis (Lepidoptera: Noctuidae). Southern blot and PCR analysis confirmed that the APA gene was integrated into the plant genome. Northern and Western blots as well as semi-quantitative agglutination assays revealed lectin expression at various levels in the transgenic lines. Biochemical analyses indicated that the recombinant APA has the same molecular structure as the native lectin. Native and recombinant lectin have the same N-terminal amino acid sequence. Bioassays using detached leaves from transgenic tobacco plants demonstrated that the ectopically expressed APA significantly (p < 0.05) reduced the weight gain of 2nd-instar larvae of S. littoralis. This inhibitory effect was observed throughout the experiment and on day 11 the caterpillars fed on transgenic tobacco leaves were 25-30% lighter in weight than the control caterpillars fed on wild type plants. In addition the lectin retarded the development of the larvae and metamorphosis, reduced pupal weight and increased mortality rate. These findings suggest that APA is a suitable insect resistance protein for integrating into plant genomes for controlling S. littoralis.https://www.eje.cz/artkey/eje-200901-0003_Laboratory_study_of_the_effects_of_leek_lectin_APA_in_transgenic_tobacco_plants_on_the_development_of_cotton.phpallium porrumalliaceaeinsect resistancelectinlepidopteraspodoptera littoralistransgenic tobacco plantweight decrease |
| spellingShingle | Amin SADEGHI Guy SMAGGHE Esmeralda JURADO-JÁCOME Willy J. PEUMANS Els J.M. VAN DAMME Laboratory study of the effects of leek lectin (APA) in transgenic tobacco plants on the development of cotton leafworm Spodoptera littoralis (Lepidoptera: Noctuidae) European Journal of Entomology allium porrum alliaceae insect resistance lectin lepidoptera spodoptera littoralis transgenic tobacco plant weight decrease |
| title | Laboratory study of the effects of leek lectin (APA) in transgenic tobacco plants on the development of cotton leafworm Spodoptera littoralis (Lepidoptera: Noctuidae) |
| title_full | Laboratory study of the effects of leek lectin (APA) in transgenic tobacco plants on the development of cotton leafworm Spodoptera littoralis (Lepidoptera: Noctuidae) |
| title_fullStr | Laboratory study of the effects of leek lectin (APA) in transgenic tobacco plants on the development of cotton leafworm Spodoptera littoralis (Lepidoptera: Noctuidae) |
| title_full_unstemmed | Laboratory study of the effects of leek lectin (APA) in transgenic tobacco plants on the development of cotton leafworm Spodoptera littoralis (Lepidoptera: Noctuidae) |
| title_short | Laboratory study of the effects of leek lectin (APA) in transgenic tobacco plants on the development of cotton leafworm Spodoptera littoralis (Lepidoptera: Noctuidae) |
| title_sort | laboratory study of the effects of leek lectin apa in transgenic tobacco plants on the development of cotton leafworm spodoptera littoralis lepidoptera noctuidae |
| topic | allium porrum alliaceae insect resistance lectin lepidoptera spodoptera littoralis transgenic tobacco plant weight decrease |
| url | https://www.eje.cz/artkey/eje-200901-0003_Laboratory_study_of_the_effects_of_leek_lectin_APA_in_transgenic_tobacco_plants_on_the_development_of_cotton.php |
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