Structural Elucidation of Novel Stable and Reactive Metabolites of Green Tea Catechins and Alkyl Gallates by LC-MS/MS
Synthetic gallic acid derivatives are employed as additives in food, personal care products, and pharmaceutical formulations. Despite their widespread use, little is known about their human exposure, health effects, and metabolism. Green tea catechins are natural antioxidants, known for their health...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/9/1635 |
| _version_ | 1827664092116025344 |
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| author | Ons Ousji Lekha Sleno |
| author_facet | Ons Ousji Lekha Sleno |
| author_sort | Ons Ousji |
| collection | DOAJ |
| description | Synthetic gallic acid derivatives are employed as additives in food, personal care products, and pharmaceutical formulations. Despite their widespread use, little is known about their human exposure, health effects, and metabolism. Green tea catechins are natural antioxidants, known for their health-promoting properties, and are also employed as food additives or in personal care products. The objective of this study was to establish metabolic pathways involved in the biotransformation of green tea catechins and synthetic gallate esters. Liquid chromatography coupled with high-resolution tandem mass spectrometry (LC-HRMS/MS) was used to elucidate oxidative and methylated metabolites, in addition to glutathione conjugates, formed in vitro using human liver microsomal incubations. The developed method was applied to 14 different parent compounds with a wide range of polarities, for the structural elucidation of many known and novel metabolites. These results serve to inform about the wide variety of possible metabolites formed upon exposure to these compounds. |
| first_indexed | 2024-03-10T00:54:22Z |
| format | Article |
| id | doaj.art-f81001e546ff4367bb3d2d36fc3e2731 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T00:54:22Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-f81001e546ff4367bb3d2d36fc3e27312023-11-23T14:46:24ZengMDPI AGAntioxidants2076-39212022-08-01119163510.3390/antiox11091635Structural Elucidation of Novel Stable and Reactive Metabolites of Green Tea Catechins and Alkyl Gallates by LC-MS/MSOns Ousji0Lekha Sleno1Chemistry Department, Université du Québec à Montréal, Downtown Station, P.O. Box 8888, Montréal, QC H3C 3P8, CanadaChemistry Department, Université du Québec à Montréal, Downtown Station, P.O. Box 8888, Montréal, QC H3C 3P8, CanadaSynthetic gallic acid derivatives are employed as additives in food, personal care products, and pharmaceutical formulations. Despite their widespread use, little is known about their human exposure, health effects, and metabolism. Green tea catechins are natural antioxidants, known for their health-promoting properties, and are also employed as food additives or in personal care products. The objective of this study was to establish metabolic pathways involved in the biotransformation of green tea catechins and synthetic gallate esters. Liquid chromatography coupled with high-resolution tandem mass spectrometry (LC-HRMS/MS) was used to elucidate oxidative and methylated metabolites, in addition to glutathione conjugates, formed in vitro using human liver microsomal incubations. The developed method was applied to 14 different parent compounds with a wide range of polarities, for the structural elucidation of many known and novel metabolites. These results serve to inform about the wide variety of possible metabolites formed upon exposure to these compounds.https://www.mdpi.com/2076-3921/11/9/1635green tea catechinsEGCGsynthetic gallatesantioxidantsmetabolitesin vitro incubations |
| spellingShingle | Ons Ousji Lekha Sleno Structural Elucidation of Novel Stable and Reactive Metabolites of Green Tea Catechins and Alkyl Gallates by LC-MS/MS Antioxidants green tea catechins EGCG synthetic gallates antioxidants metabolites in vitro incubations |
| title | Structural Elucidation of Novel Stable and Reactive Metabolites of Green Tea Catechins and Alkyl Gallates by LC-MS/MS |
| title_full | Structural Elucidation of Novel Stable and Reactive Metabolites of Green Tea Catechins and Alkyl Gallates by LC-MS/MS |
| title_fullStr | Structural Elucidation of Novel Stable and Reactive Metabolites of Green Tea Catechins and Alkyl Gallates by LC-MS/MS |
| title_full_unstemmed | Structural Elucidation of Novel Stable and Reactive Metabolites of Green Tea Catechins and Alkyl Gallates by LC-MS/MS |
| title_short | Structural Elucidation of Novel Stable and Reactive Metabolites of Green Tea Catechins and Alkyl Gallates by LC-MS/MS |
| title_sort | structural elucidation of novel stable and reactive metabolites of green tea catechins and alkyl gallates by lc ms ms |
| topic | green tea catechins EGCG synthetic gallates antioxidants metabolites in vitro incubations |
| url | https://www.mdpi.com/2076-3921/11/9/1635 |
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