Antiproliferative Effects of Pancratium Maritimum Extracts on Normal and Cancerous Cells
Background: Plants are an important natural source of compounds used in cancer therapy. Pancratium maritimum contains potential anti-cancer agents such as alkaloids. In this study, we investigated the anti-proliferative effects of P. maritimum extracts on MDA-MB-231 human epithelial adenocarcinoma c...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2018-01-01
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| Series: | Iranian Journal of Medical Sciences |
| Subjects: | |
| Online Access: | http://ijms.sums.ac.ir/index.php/IJMS/article/view/3212 |
| _version_ | 1818319720152039424 |
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| author | Ghaleb Tayoub Mohmad Al-Odat Amal Amer Abdulmunim Aljapawe Adnan Ekhtiar |
| author_facet | Ghaleb Tayoub Mohmad Al-Odat Amal Amer Abdulmunim Aljapawe Adnan Ekhtiar |
| author_sort | Ghaleb Tayoub |
| collection | DOAJ |
| description | Background: Plants are an important natural source of compounds used in cancer therapy. Pancratium maritimum contains potential anti-cancer agents such as alkaloids. In this study, we investigated the anti-proliferative effects of P. maritimum extracts on MDA-MB-231 human epithelial adenocarcinoma cell line and on normal lymphocytes in vitro. Methods: Leaves, flowers, roots, and bulbs of P. maritimum were collected and their contents were extracted and diluted to different concentrations that were applied on MDA-MB-231 cells and normal human lymphocytes in vitro for different intervals. Cells viability, proliferation, cell cycle distribution, apoptosis, and growth were evaluated by flow cytometry and microscopy. Parametric unpaired t-test was used to compare effects of plant extracts on treated cell cultures with untreated control cell cultures. IC50 was also calculated.
Results: P. maritimum extract had profound effects on MDA-MB-321 cells. It inhibited cell proliferation in a dose- and time-dependent manner. The IC50 values were 0.039, 0.035, and 0.026 mg/ml after 48, 72, and 96 hours of treatment with 0.1 mg/ml concentration of bulb extract, respectively. Those values were 0.051 and 0.03 mg/ml after 72 and 96 hours for root extract, respectively, and 0.048 mg/ml after 96 hours for flower extract. There were no significant effects of P. maritimum bulb extracts on normal lymphocytes proliferation.
Conclusion: P. maritimum extract has anti-proliferative effects on MDA-MB-231 cell line in vitro. The effects imply the involvement of mechanisms that inhibits cell growth and arresting cells at S and G2/M phases. Cyclin B1, Bcl-2, and Ki67 expression was also affected. |
| first_indexed | 2024-12-13T10:13:36Z |
| format | Article |
| id | doaj.art-f81504b6a78d4cb19572e1924fe11e7e |
| institution | Directory Open Access Journal |
| issn | 0253-0716 1735-3688 |
| language | English |
| last_indexed | 2024-12-13T10:13:36Z |
| publishDate | 2018-01-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Medical Sciences |
| spelling | doaj.art-f81504b6a78d4cb19572e1924fe11e7e2022-12-21T23:51:21ZengShiraz University of Medical SciencesIranian Journal of Medical Sciences0253-07161735-36882018-01-014315264Antiproliferative Effects of Pancratium Maritimum Extracts on Normal and Cancerous CellsGhaleb Tayoub0Mohmad Al-Odat1Amal Amer2Abdulmunim Aljapawe3Adnan Ekhtiar4Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria, Damascus, SyriaDepartment of Radiation Protection and Safety, Atomic Energy Commission of Syria, Damascus, SyriaDepartment of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria, Damascus, SyriaDepartment of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria, Damascus, SyriaDepartment of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria, Damascus, SyriaBackground: Plants are an important natural source of compounds used in cancer therapy. Pancratium maritimum contains potential anti-cancer agents such as alkaloids. In this study, we investigated the anti-proliferative effects of P. maritimum extracts on MDA-MB-231 human epithelial adenocarcinoma cell line and on normal lymphocytes in vitro. Methods: Leaves, flowers, roots, and bulbs of P. maritimum were collected and their contents were extracted and diluted to different concentrations that were applied on MDA-MB-231 cells and normal human lymphocytes in vitro for different intervals. Cells viability, proliferation, cell cycle distribution, apoptosis, and growth were evaluated by flow cytometry and microscopy. Parametric unpaired t-test was used to compare effects of plant extracts on treated cell cultures with untreated control cell cultures. IC50 was also calculated. Results: P. maritimum extract had profound effects on MDA-MB-321 cells. It inhibited cell proliferation in a dose- and time-dependent manner. The IC50 values were 0.039, 0.035, and 0.026 mg/ml after 48, 72, and 96 hours of treatment with 0.1 mg/ml concentration of bulb extract, respectively. Those values were 0.051 and 0.03 mg/ml after 72 and 96 hours for root extract, respectively, and 0.048 mg/ml after 96 hours for flower extract. There were no significant effects of P. maritimum bulb extracts on normal lymphocytes proliferation. Conclusion: P. maritimum extract has anti-proliferative effects on MDA-MB-231 cell line in vitro. The effects imply the involvement of mechanisms that inhibits cell growth and arresting cells at S and G2/M phases. Cyclin B1, Bcl-2, and Ki67 expression was also affected.http://ijms.sums.ac.ir/index.php/IJMS/article/view/3212MDA-MB-231Pancratium maritimumGene ExpressionCell ProliferationLymphocytes |
| spellingShingle | Ghaleb Tayoub Mohmad Al-Odat Amal Amer Abdulmunim Aljapawe Adnan Ekhtiar Antiproliferative Effects of Pancratium Maritimum Extracts on Normal and Cancerous Cells Iranian Journal of Medical Sciences MDA-MB-231 Pancratium maritimum Gene Expression Cell Proliferation Lymphocytes |
| title | Antiproliferative Effects of Pancratium Maritimum Extracts on Normal and Cancerous Cells |
| title_full | Antiproliferative Effects of Pancratium Maritimum Extracts on Normal and Cancerous Cells |
| title_fullStr | Antiproliferative Effects of Pancratium Maritimum Extracts on Normal and Cancerous Cells |
| title_full_unstemmed | Antiproliferative Effects of Pancratium Maritimum Extracts on Normal and Cancerous Cells |
| title_short | Antiproliferative Effects of Pancratium Maritimum Extracts on Normal and Cancerous Cells |
| title_sort | antiproliferative effects of pancratium maritimum extracts on normal and cancerous cells |
| topic | MDA-MB-231 Pancratium maritimum Gene Expression Cell Proliferation Lymphocytes |
| url | http://ijms.sums.ac.ir/index.php/IJMS/article/view/3212 |
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