Osteogenic Differentiation of Human Dental Pulp-derived Stem Cells under Various Ex-vivo Culture Conditions
Dental pulp stem cells (DPSCs) can be easily isolated and cultured in low-serum containing medium supplemented with growth factors PDGF-BB and EGF while exhibiting multipotency and immature phenotypic characteristics. In the present study, we investigated their potential to differentiate towards ost...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Karolinum Press
2010-01-01
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| Series: | Acta Medica |
| Subjects: | |
| Online Access: | https://actamedica.lfhk.cuni.cz/53/2/0079/ |
| _version_ | 1819153174711238656 |
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| author | Jana Karbanová Tomáš Soukup Jakub Suchánek Jaroslav Mokrý |
| author_facet | Jana Karbanová Tomáš Soukup Jakub Suchánek Jaroslav Mokrý |
| author_sort | Jana Karbanová |
| collection | DOAJ |
| description | Dental pulp stem cells (DPSCs) can be easily isolated and cultured in low-serum containing medium supplemented with growth factors PDGF-BB and EGF while exhibiting multipotency and immature phenotypic characteristics. In the present study, we investigated their potential to differentiate towards osteogenic lineages using various culture conditions in order to optimize their therapeutic use. DPSCs were cultured either as a cell monolayer or as three-dimensional (3D) micro-mass structures. Monolayers preincubated with bFGF and valproic acid for one week prior their differentiation were cultured in serum containing standard osteodifferentiation medium for four weeks, which resulted in multilayered nodule formation. Micro-mass structures were cultured for same period either in serum containing medium or under serum-free conditions supplemented with TGF-β3 with or without BMP-2. Histochemically, we detected massive collagen I and weak calcium phosphate depositions in multilayered nodules. When culture 3D-aggregates in either standard osteodifferentiation medium or serum-free medium containing TGF-β3, only small amount of collagen I fibres was observed and almost no deposits of calcium phosphate were detected. In contrast, in presence of both TGF-β3 and BMP-2 in the serum- free medium a significant amount of collagen I fibers/bundles and calcification were detected, which is in line with osteogenic effect of BMP-2. Thus, our data indicate that certain environmental cues can enhance differentiation process of DPSCs into osteogenic lineage, which suggest their possible utilization in tissue engineering. |
| first_indexed | 2024-12-22T15:01:00Z |
| format | Article |
| id | doaj.art-f8177df11c9e4fb8b467494616a5762a |
| institution | Directory Open Access Journal |
| issn | 1211-4286 1805-9694 |
| language | English |
| last_indexed | 2024-12-22T15:01:00Z |
| publishDate | 2010-01-01 |
| publisher | Karolinum Press |
| record_format | Article |
| series | Acta Medica |
| spelling | doaj.art-f8177df11c9e4fb8b467494616a5762a2022-12-21T18:22:07ZengKarolinum PressActa Medica1211-42861805-96942010-01-01532798410.14712/18059694.2016.64Osteogenic Differentiation of Human Dental Pulp-derived Stem Cells under Various Ex-vivo Culture ConditionsJana Karbanová0Tomáš Soukup1Jakub Suchánek2Jaroslav Mokrý3Charles University in Prague, Faculty of Medicine and University Hospital Hradec Králové, Department of Histology and Embryology, Hradec Králové, Czech Republic; Tissue Engineering Laboratories, BIOTEC, Technische Universität Dresden, GermanyCharles University in Prague, Faculty of Medicine and University Hospital Hradec Králové, Department of Histology and Embryology, Hradec Králové, Czech RepublicCharles University in Prague, Faculty of Medicine and University Hospital Hradec Králové, Department of Dentistry, Hradec Králové, Czech RepublicCharles University in Prague, Faculty of Medicine and University Hospital Hradec Králové, Department of Histology and Embryology, Hradec Králové, Czech RepublicDental pulp stem cells (DPSCs) can be easily isolated and cultured in low-serum containing medium supplemented with growth factors PDGF-BB and EGF while exhibiting multipotency and immature phenotypic characteristics. In the present study, we investigated their potential to differentiate towards osteogenic lineages using various culture conditions in order to optimize their therapeutic use. DPSCs were cultured either as a cell monolayer or as three-dimensional (3D) micro-mass structures. Monolayers preincubated with bFGF and valproic acid for one week prior their differentiation were cultured in serum containing standard osteodifferentiation medium for four weeks, which resulted in multilayered nodule formation. Micro-mass structures were cultured for same period either in serum containing medium or under serum-free conditions supplemented with TGF-β3 with or without BMP-2. Histochemically, we detected massive collagen I and weak calcium phosphate depositions in multilayered nodules. When culture 3D-aggregates in either standard osteodifferentiation medium or serum-free medium containing TGF-β3, only small amount of collagen I fibres was observed and almost no deposits of calcium phosphate were detected. In contrast, in presence of both TGF-β3 and BMP-2 in the serum- free medium a significant amount of collagen I fibers/bundles and calcification were detected, which is in line with osteogenic effect of BMP-2. Thus, our data indicate that certain environmental cues can enhance differentiation process of DPSCs into osteogenic lineage, which suggest their possible utilization in tissue engineering.https://actamedica.lfhk.cuni.cz/53/2/0079/Dental pulp stem cellsOsteogenic differentiationValproic acidBMP-2Micro-mass culture |
| spellingShingle | Jana Karbanová Tomáš Soukup Jakub Suchánek Jaroslav Mokrý Osteogenic Differentiation of Human Dental Pulp-derived Stem Cells under Various Ex-vivo Culture Conditions Acta Medica Dental pulp stem cells Osteogenic differentiation Valproic acid BMP-2 Micro-mass culture |
| title | Osteogenic Differentiation of Human Dental Pulp-derived Stem Cells under Various Ex-vivo Culture Conditions |
| title_full | Osteogenic Differentiation of Human Dental Pulp-derived Stem Cells under Various Ex-vivo Culture Conditions |
| title_fullStr | Osteogenic Differentiation of Human Dental Pulp-derived Stem Cells under Various Ex-vivo Culture Conditions |
| title_full_unstemmed | Osteogenic Differentiation of Human Dental Pulp-derived Stem Cells under Various Ex-vivo Culture Conditions |
| title_short | Osteogenic Differentiation of Human Dental Pulp-derived Stem Cells under Various Ex-vivo Culture Conditions |
| title_sort | osteogenic differentiation of human dental pulp derived stem cells under various ex vivo culture conditions |
| topic | Dental pulp stem cells Osteogenic differentiation Valproic acid BMP-2 Micro-mass culture |
| url | https://actamedica.lfhk.cuni.cz/53/2/0079/ |
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