Orphan response regulator CovR plays positive regulative functions in the survivability and pathogenicity of Streptococcus suis serotype 2 isolated from a pig
Abstract Background Streptococcus suis serotype 2 (S. suis 2) is an important zoonotic pathogen. Orphan response regulator CovR plays crucial regulative functions in the survivability and pathogenicity of S. suis 2. However, research on the CovR in S. suis 2 is limited. Results In this study, the re...
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-023-03808-9 |
| _version_ | 1797452279057481728 |
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| author | Yanyan Zhang Rui Li Qian Li Yongwei Zhu Xiaopei Yang Di Zhao Bingbing Zong |
| author_facet | Yanyan Zhang Rui Li Qian Li Yongwei Zhu Xiaopei Yang Di Zhao Bingbing Zong |
| author_sort | Yanyan Zhang |
| collection | DOAJ |
| description | Abstract Background Streptococcus suis serotype 2 (S. suis 2) is an important zoonotic pathogen. Orphan response regulator CovR plays crucial regulative functions in the survivability and pathogenicity of S. suis 2. However, research on the CovR in S. suis 2 is limited. Results In this study, the regulative functions of CovR in the survivability and pathogenicity were investigated in S. suis 2 isolated from a diseased pig. The deletion of CovR significantly weakened the survivability and pathogenicity of S. suis 2. Compared with the wild-type strain, ΔcovR showed slower growth rates and thinner capsular polysaccharides. Moreover, ΔcovR showed reduced adhesion and invasion to Hep-2 cells as well as anti-phagocytosis and anti-killing ability to 3D4 cells and anti-serum killing ability. In addition, the deletion of CovR significantly reduced the colonisation ability of S. suis 2 in mice. The survival rate of mice infected with ΔcovR was increased by 16.7% compared with that of mice infected with S. suis 2. Further, the deletion of CovR led to dramatic changes in metabolism-related pathways in S. suis 2, five of those, including fructose and mannose metabolism, glycerolipid metabolism, ABC transporters, amino sugar and nucleotide sugar metabolism and phosphotransferase system, were significantly down-regulated. Conclusions Based on the results, CovR plays positive regulative functions in the survivability and pathogenicity of S. suis 2 SC19 strain isolated from a pig. |
| first_indexed | 2024-03-09T15:06:27Z |
| format | Article |
| id | doaj.art-f81b8c97f4d0487aad0c0ce0ec283401 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-03-09T15:06:27Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-f81b8c97f4d0487aad0c0ce0ec2834012023-11-26T13:38:06ZengBMCBMC Veterinary Research1746-61482023-11-0119111510.1186/s12917-023-03808-9Orphan response regulator CovR plays positive regulative functions in the survivability and pathogenicity of Streptococcus suis serotype 2 isolated from a pigYanyan Zhang0Rui Li1Qian Li2Yongwei Zhu3Xiaopei Yang4Di Zhao5Bingbing Zong6Hubei Key Laboratory of Animal Nutrition and Feed Science, Engineering Research Center of Feed Protein Resources on Agricultural By-products, Ministry of Education, Wuhan Polytechnic UniversityHubei Key Laboratory of Animal Nutrition and Feed Science, Engineering Research Center of Feed Protein Resources on Agricultural By-products, Ministry of Education, Wuhan Polytechnic UniversityHubei Key Laboratory of Animal Nutrition and Feed Science, Engineering Research Center of Feed Protein Resources on Agricultural By-products, Ministry of Education, Wuhan Polytechnic UniversityHunan International Scientific and Technological Cooperation Base of Brain Tumor Research, Xiangya Hospital, Central South UniversityWuhan animal disease control centerHubei Key Laboratory of Animal Nutrition and Feed Science, Engineering Research Center of Feed Protein Resources on Agricultural By-products, Ministry of Education, Wuhan Polytechnic UniversityHubei Key Laboratory of Animal Nutrition and Feed Science, Engineering Research Center of Feed Protein Resources on Agricultural By-products, Ministry of Education, Wuhan Polytechnic UniversityAbstract Background Streptococcus suis serotype 2 (S. suis 2) is an important zoonotic pathogen. Orphan response regulator CovR plays crucial regulative functions in the survivability and pathogenicity of S. suis 2. However, research on the CovR in S. suis 2 is limited. Results In this study, the regulative functions of CovR in the survivability and pathogenicity were investigated in S. suis 2 isolated from a diseased pig. The deletion of CovR significantly weakened the survivability and pathogenicity of S. suis 2. Compared with the wild-type strain, ΔcovR showed slower growth rates and thinner capsular polysaccharides. Moreover, ΔcovR showed reduced adhesion and invasion to Hep-2 cells as well as anti-phagocytosis and anti-killing ability to 3D4 cells and anti-serum killing ability. In addition, the deletion of CovR significantly reduced the colonisation ability of S. suis 2 in mice. The survival rate of mice infected with ΔcovR was increased by 16.7% compared with that of mice infected with S. suis 2. Further, the deletion of CovR led to dramatic changes in metabolism-related pathways in S. suis 2, five of those, including fructose and mannose metabolism, glycerolipid metabolism, ABC transporters, amino sugar and nucleotide sugar metabolism and phosphotransferase system, were significantly down-regulated. Conclusions Based on the results, CovR plays positive regulative functions in the survivability and pathogenicity of S. suis 2 SC19 strain isolated from a pig.https://doi.org/10.1186/s12917-023-03808-9Streptococcus suis type 2Orphan response regulator CovRPathogenicityRegulative functionMetabolic pathway |
| spellingShingle | Yanyan Zhang Rui Li Qian Li Yongwei Zhu Xiaopei Yang Di Zhao Bingbing Zong Orphan response regulator CovR plays positive regulative functions in the survivability and pathogenicity of Streptococcus suis serotype 2 isolated from a pig BMC Veterinary Research Streptococcus suis type 2 Orphan response regulator CovR Pathogenicity Regulative function Metabolic pathway |
| title | Orphan response regulator CovR plays positive regulative functions in the survivability and pathogenicity of Streptococcus suis serotype 2 isolated from a pig |
| title_full | Orphan response regulator CovR plays positive regulative functions in the survivability and pathogenicity of Streptococcus suis serotype 2 isolated from a pig |
| title_fullStr | Orphan response regulator CovR plays positive regulative functions in the survivability and pathogenicity of Streptococcus suis serotype 2 isolated from a pig |
| title_full_unstemmed | Orphan response regulator CovR plays positive regulative functions in the survivability and pathogenicity of Streptococcus suis serotype 2 isolated from a pig |
| title_short | Orphan response regulator CovR plays positive regulative functions in the survivability and pathogenicity of Streptococcus suis serotype 2 isolated from a pig |
| title_sort | orphan response regulator covr plays positive regulative functions in the survivability and pathogenicity of streptococcus suis serotype 2 isolated from a pig |
| topic | Streptococcus suis type 2 Orphan response regulator CovR Pathogenicity Regulative function Metabolic pathway |
| url | https://doi.org/10.1186/s12917-023-03808-9 |
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