A Feasibility Study on the Recall of Metallophilic Fungi from Fe(III)-Contaminated Soil and Evaluating Their Mycoremediation Capacity: Experimental and Theoretical Study
Mycoremediation is one of the most attractive, eco-friendly, and sustainable methods to mitigate the toxic effects of heavy metals. This study aimed to determine the mycoremediation capacity of metallophilic fungi isolated from heavy-metal-contaminated soil containing a high Fe(III) concentration (1...
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MDPI AG
2023-03-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/3/382 |
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| author | Aya I. Tagyan Manal M. Yasser Ahmed M. Mousa Dalal Hussien M. Alkhalifah Wael N. Hozzein Marym A. Marzouk |
| author_facet | Aya I. Tagyan Manal M. Yasser Ahmed M. Mousa Dalal Hussien M. Alkhalifah Wael N. Hozzein Marym A. Marzouk |
| author_sort | Aya I. Tagyan |
| collection | DOAJ |
| description | Mycoremediation is one of the most attractive, eco-friendly, and sustainable methods to mitigate the toxic effects of heavy metals. This study aimed to determine the mycoremediation capacity of metallophilic fungi isolated from heavy-metal-contaminated soil containing a high Fe(III) concentration (118.40 mg/kg). Four common fungal strains were isolated, including <i>Curvularia lunata</i>, <i>Fusarium equiseti</i>, <i>Penicillium pinophilum</i>, and <i>Trichoderma harzianum</i>. These fungal strains were exposed to gradually increasing concentrations of Fe(III) of 100, 200, 300, 400, 500, 600, 700, 800, 900, and 1000 mg/L. Sophisticated techniques and tests were employed to investigate the mycoremediation capability, including tolerance index (TI), scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and adsorption isotherm. Furthermore, the impacts of initial concentration, pH, and temperature on the Fe(III) removal (%) and uptake capacity (mg/g) of the studied samples were investigated. The results were validated by statistical analysis using one-way ANOVA. It was found that the Fe(III) uptake with different ratios triggered alterations in the Fe(III) tolerance (TI) morphological (SEM), chemical (FTIR), and adsorption capacity properties. The highest Fe(III) tolerance for all studied fungal strains was achieved at 100 mg/L. Moreover, the optimum conditions of Fe(III) removal (%) for all studied fungal strains were within pH 7 and 28 °C, with similar performance at the initial Fe(III) concentration ranging from 50–200 mg/L. At the same time, the maximum Fe(III) uptake was achieved at pH 7, 20 °C, and 200 mg/L. Compared to other strains, the Fe(III) tolerance of <i>T. harzianum</i> was rise in the Fe(III) concentration. The Fe(III) uptake reaction was corroborated by best fitting with the Langmuir model, achieving optimum adsorption capacities of 61.34, 62.90, 63.30, and 72.46 mg/g for <i>C.lunata</i>, <i>F. equiseti</i>, <i>P. pinophilum</i>, <i>T. harzianum</i>, respectively. It can be deduced that the addressed fungi species can be applied in mycoremediation according to the utilized Fe(III) concentrations with more superiority for live <i>T. harzianum</i>. |
| first_indexed | 2024-03-11T06:20:42Z |
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| id | doaj.art-f81cdb77f65a4ecebb98d664f4ff6e97 |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-11T06:20:42Z |
| publishDate | 2023-03-01 |
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| spelling | doaj.art-f81cdb77f65a4ecebb98d664f4ff6e972023-11-17T12:00:47ZengMDPI AGJournal of Fungi2309-608X2023-03-019338210.3390/jof9030382A Feasibility Study on the Recall of Metallophilic Fungi from Fe(III)-Contaminated Soil and Evaluating Their Mycoremediation Capacity: Experimental and Theoretical StudyAya I. Tagyan0Manal M. Yasser1Ahmed M. Mousa2Dalal Hussien M. Alkhalifah3Wael N. Hozzein4Marym A. Marzouk5Department of Botany and Microbiology, Faculty of Science, Beni-Suef University, Beni-Suef 62511, EgyptDepartment of Botany and Microbiology, Faculty of Science, Beni-Suef University, Beni-Suef 62511, EgyptDepartment of Botany and Microbiology, Faculty of Science, Beni-Suef University, Beni-Suef 62511, EgyptDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaDepartment of Botany and Microbiology, Faculty of Science, Beni-Suef University, Beni-Suef 62511, EgyptDepartment of Botany and Microbiology, Faculty of Science, Beni-Suef University, Beni-Suef 62511, EgyptMycoremediation is one of the most attractive, eco-friendly, and sustainable methods to mitigate the toxic effects of heavy metals. This study aimed to determine the mycoremediation capacity of metallophilic fungi isolated from heavy-metal-contaminated soil containing a high Fe(III) concentration (118.40 mg/kg). Four common fungal strains were isolated, including <i>Curvularia lunata</i>, <i>Fusarium equiseti</i>, <i>Penicillium pinophilum</i>, and <i>Trichoderma harzianum</i>. These fungal strains were exposed to gradually increasing concentrations of Fe(III) of 100, 200, 300, 400, 500, 600, 700, 800, 900, and 1000 mg/L. Sophisticated techniques and tests were employed to investigate the mycoremediation capability, including tolerance index (TI), scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and adsorption isotherm. Furthermore, the impacts of initial concentration, pH, and temperature on the Fe(III) removal (%) and uptake capacity (mg/g) of the studied samples were investigated. The results were validated by statistical analysis using one-way ANOVA. It was found that the Fe(III) uptake with different ratios triggered alterations in the Fe(III) tolerance (TI) morphological (SEM), chemical (FTIR), and adsorption capacity properties. The highest Fe(III) tolerance for all studied fungal strains was achieved at 100 mg/L. Moreover, the optimum conditions of Fe(III) removal (%) for all studied fungal strains were within pH 7 and 28 °C, with similar performance at the initial Fe(III) concentration ranging from 50–200 mg/L. At the same time, the maximum Fe(III) uptake was achieved at pH 7, 20 °C, and 200 mg/L. Compared to other strains, the Fe(III) tolerance of <i>T. harzianum</i> was rise in the Fe(III) concentration. The Fe(III) uptake reaction was corroborated by best fitting with the Langmuir model, achieving optimum adsorption capacities of 61.34, 62.90, 63.30, and 72.46 mg/g for <i>C.lunata</i>, <i>F. equiseti</i>, <i>P. pinophilum</i>, <i>T. harzianum</i>, respectively. It can be deduced that the addressed fungi species can be applied in mycoremediation according to the utilized Fe(III) concentrations with more superiority for live <i>T. harzianum</i>.https://www.mdpi.com/2309-608X/9/3/382Fe(III)metallophilic fungi<i>Trichoderma harzianum</i>mycoremediationsoil |
| spellingShingle | Aya I. Tagyan Manal M. Yasser Ahmed M. Mousa Dalal Hussien M. Alkhalifah Wael N. Hozzein Marym A. Marzouk A Feasibility Study on the Recall of Metallophilic Fungi from Fe(III)-Contaminated Soil and Evaluating Their Mycoremediation Capacity: Experimental and Theoretical Study Journal of Fungi Fe(III) metallophilic fungi <i>Trichoderma harzianum</i> mycoremediation soil |
| title | A Feasibility Study on the Recall of Metallophilic Fungi from Fe(III)-Contaminated Soil and Evaluating Their Mycoremediation Capacity: Experimental and Theoretical Study |
| title_full | A Feasibility Study on the Recall of Metallophilic Fungi from Fe(III)-Contaminated Soil and Evaluating Their Mycoremediation Capacity: Experimental and Theoretical Study |
| title_fullStr | A Feasibility Study on the Recall of Metallophilic Fungi from Fe(III)-Contaminated Soil and Evaluating Their Mycoremediation Capacity: Experimental and Theoretical Study |
| title_full_unstemmed | A Feasibility Study on the Recall of Metallophilic Fungi from Fe(III)-Contaminated Soil and Evaluating Their Mycoremediation Capacity: Experimental and Theoretical Study |
| title_short | A Feasibility Study on the Recall of Metallophilic Fungi from Fe(III)-Contaminated Soil and Evaluating Their Mycoremediation Capacity: Experimental and Theoretical Study |
| title_sort | feasibility study on the recall of metallophilic fungi from fe iii contaminated soil and evaluating their mycoremediation capacity experimental and theoretical study |
| topic | Fe(III) metallophilic fungi <i>Trichoderma harzianum</i> mycoremediation soil |
| url | https://www.mdpi.com/2309-608X/9/3/382 |
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