Functional and Immunofluorescence Evaluations of Vascular and Neural Integrities in Urinary Bladder of Streptozotocin-Induced Diabetic Mice
Purpose To assess functional and structural changes in vascular and neural structures associated with diabetic bladder dysfunction (DBD) in the bladders of streptozotocin (STZ)-induced diabetic mice. Methods Eight-week-old C57BL/6 mice were injected with STZ at 50 mg/kg daily for 5 consecutive days....
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| Format: | Article |
| Language: | English |
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Korean Continence Society
2022-09-01
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| Series: | International Neurourology Journal |
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| Online Access: | http://einj.org/upload/pdf/inj-2244152-076.pdf |
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| author | Mi-Hye Kwon Min-Ji Choi Fang-Yuan Liu Fitri Rahma Fridayana Lashkari Niloofar Guo Nan Yin Ji-Kan Ryu |
| author_facet | Mi-Hye Kwon Min-Ji Choi Fang-Yuan Liu Fitri Rahma Fridayana Lashkari Niloofar Guo Nan Yin Ji-Kan Ryu |
| author_sort | Mi-Hye Kwon |
| collection | DOAJ |
| description | Purpose To assess functional and structural changes in vascular and neural structures associated with diabetic bladder dysfunction (DBD) in the bladders of streptozotocin (STZ)-induced diabetic mice. Methods Eight-week-old C57BL/6 mice were injected with STZ at 50 mg/kg daily for 5 consecutive days. Catheters were inserted 12 weeks later, and 5 days after catheter placement bladder functions were assessed by conscious cystometry. Neurovascular and extracellular matrix marker changes in harvested urinary bladders were investigated by immunofluorescent staining. Body weights and fasting and postprandial blood glucose levels were measured 12 weeks after STZ injection. Results STZ-induced diabetic mice had significantly lower body weights and significantly higher blood glucose levels. Assessment of bladder function in STZ-induced diabetic mice revealed a nearly 3-fold increase in bladder capacity and intercontractile interval compared to controls. However, basal pressure, maximal bladder pressure, and threshold pressure were not significantly different. Morphological and structural analysis showed that STZ-induced diabetic mice had significantly reduced microvascular density in lamina propria (33% of the nondiabetic control values), and severely decreased nerve contents in the detrusor region (42% of the nondiabetic control values). Conclusions STZ-induced diabetic mice exhibit functional and structural derangements in urinary bladder. The present study provides a foundation and describes a useful means of evaluating the efficacies of therapeutic targets and exploring the detailed mechanism of DBD. |
| first_indexed | 2024-04-13T19:53:19Z |
| format | Article |
| id | doaj.art-f81e4269336f4c759a20d92d51e613ee |
| institution | Directory Open Access Journal |
| issn | 2093-4777 2093-6931 |
| language | English |
| last_indexed | 2024-04-13T19:53:19Z |
| publishDate | 2022-09-01 |
| publisher | Korean Continence Society |
| record_format | Article |
| series | International Neurourology Journal |
| spelling | doaj.art-f81e4269336f4c759a20d92d51e613ee2022-12-22T02:32:26ZengKorean Continence SocietyInternational Neurourology Journal2093-47772093-69312022-09-0126320120910.5213/inj.2244152.0761011Functional and Immunofluorescence Evaluations of Vascular and Neural Integrities in Urinary Bladder of Streptozotocin-Induced Diabetic MiceMi-Hye Kwon0Min-Ji Choi1Fang-Yuan Liu2Fitri Rahma Fridayana3Lashkari Niloofar4Guo Nan YinJi-Kan Ryu5 Department of Urology, Inha University College of Medicine, National Research Center for Sexual Medicine, Incheon, Korea Department of Urology, Inha University College of Medicine, National Research Center for Sexual Medicine, Incheon, Korea Department of Urology, Inha University College of Medicine, National Research Center for Sexual Medicine, Incheon, Korea Department of Urology, Inha University College of Medicine, National Research Center for Sexual Medicine, Incheon, Korea Department of Urology, Inha University College of Medicine, National Research Center for Sexual Medicine, Incheon, Korea Department of Urology, Inha University College of Medicine, National Research Center for Sexual Medicine, Incheon, KoreaPurpose To assess functional and structural changes in vascular and neural structures associated with diabetic bladder dysfunction (DBD) in the bladders of streptozotocin (STZ)-induced diabetic mice. Methods Eight-week-old C57BL/6 mice were injected with STZ at 50 mg/kg daily for 5 consecutive days. Catheters were inserted 12 weeks later, and 5 days after catheter placement bladder functions were assessed by conscious cystometry. Neurovascular and extracellular matrix marker changes in harvested urinary bladders were investigated by immunofluorescent staining. Body weights and fasting and postprandial blood glucose levels were measured 12 weeks after STZ injection. Results STZ-induced diabetic mice had significantly lower body weights and significantly higher blood glucose levels. Assessment of bladder function in STZ-induced diabetic mice revealed a nearly 3-fold increase in bladder capacity and intercontractile interval compared to controls. However, basal pressure, maximal bladder pressure, and threshold pressure were not significantly different. Morphological and structural analysis showed that STZ-induced diabetic mice had significantly reduced microvascular density in lamina propria (33% of the nondiabetic control values), and severely decreased nerve contents in the detrusor region (42% of the nondiabetic control values). Conclusions STZ-induced diabetic mice exhibit functional and structural derangements in urinary bladder. The present study provides a foundation and describes a useful means of evaluating the efficacies of therapeutic targets and exploring the detailed mechanism of DBD.http://einj.org/upload/pdf/inj-2244152-076.pdfurinary bladderblood vesselnervediabetes mellitusmouse |
| spellingShingle | Mi-Hye Kwon Min-Ji Choi Fang-Yuan Liu Fitri Rahma Fridayana Lashkari Niloofar Guo Nan Yin Ji-Kan Ryu Functional and Immunofluorescence Evaluations of Vascular and Neural Integrities in Urinary Bladder of Streptozotocin-Induced Diabetic Mice International Neurourology Journal urinary bladder blood vessel nerve diabetes mellitus mouse |
| title | Functional and Immunofluorescence Evaluations of Vascular and Neural Integrities in Urinary Bladder of Streptozotocin-Induced Diabetic Mice |
| title_full | Functional and Immunofluorescence Evaluations of Vascular and Neural Integrities in Urinary Bladder of Streptozotocin-Induced Diabetic Mice |
| title_fullStr | Functional and Immunofluorescence Evaluations of Vascular and Neural Integrities in Urinary Bladder of Streptozotocin-Induced Diabetic Mice |
| title_full_unstemmed | Functional and Immunofluorescence Evaluations of Vascular and Neural Integrities in Urinary Bladder of Streptozotocin-Induced Diabetic Mice |
| title_short | Functional and Immunofluorescence Evaluations of Vascular and Neural Integrities in Urinary Bladder of Streptozotocin-Induced Diabetic Mice |
| title_sort | functional and immunofluorescence evaluations of vascular and neural integrities in urinary bladder of streptozotocin induced diabetic mice |
| topic | urinary bladder blood vessel nerve diabetes mellitus mouse |
| url | http://einj.org/upload/pdf/inj-2244152-076.pdf |
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