Serum Autoantibody Biomarkers for Management of Rheumatoid Arthritis Disease
Rheumatoid arthritis (RA) is a systemic chronic autoimmune inflammatory disease that is characterized by the destruction of bone and production of autoantibodies such as rheumatoid factor (RF) and anticitrullinated protein antibodies (ACPAs). The high prevalence of this disease and the need of affor...
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MDPI AG
2023-03-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/3/381 |
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| author | Esther Sánchez-Tirado Lourdes Agüí Marta Sánchez-Paniagua Araceli González-Cortés Beatriz López-Ruiz Paloma Yáñez-Sedeño José M. Pingarrón |
| author_facet | Esther Sánchez-Tirado Lourdes Agüí Marta Sánchez-Paniagua Araceli González-Cortés Beatriz López-Ruiz Paloma Yáñez-Sedeño José M. Pingarrón |
| author_sort | Esther Sánchez-Tirado |
| collection | DOAJ |
| description | Rheumatoid arthritis (RA) is a systemic chronic autoimmune inflammatory disease that is characterized by the destruction of bone and production of autoantibodies such as rheumatoid factor (RF) and anticitrullinated protein antibodies (ACPAs). The high prevalence of this disease and the need of affordable tools for its early detection led us to prepare the first electrochemical immunoplatform for the simultaneous determination of four RA biomarkers, the autoantibodies: RF, anti-peptidyl-arginine deiminase enzyme (anti-PAD4), anti-cyclic citrullinated peptide (anti-CCP), and anti-citrullinated vimentin (anti-MCV). Functionalized magnetic beads (MBs) were used to immobilize the specific antigens, and sandwich-type immunoassays were implemented for the amperometric detection of the four autoantibodies, using the horseradish peroxidase (HRP)/H<sub>2</sub>O<sub>2</sub>/hydroquinone (HQ) system. The immunoplatform was applied to the determination of the biomarkers in human serum of twenty-two patients diagnosed with RA and four healthy individuals, and the results were validated against ELISA tests and the certified values. |
| first_indexed | 2024-03-11T06:52:08Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-11T06:52:08Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
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| series | Biosensors |
| spelling | doaj.art-f8208b109327489dbf55026024bc8fba2023-11-17T09:54:34ZengMDPI AGBiosensors2079-63742023-03-0113338110.3390/bios13030381Serum Autoantibody Biomarkers for Management of Rheumatoid Arthritis DiseaseEsther Sánchez-Tirado0Lourdes Agüí1Marta Sánchez-Paniagua2Araceli González-Cortés3Beatriz López-Ruiz4Paloma Yáñez-Sedeño5José M. Pingarrón6Department of Analytical Chemistry, Faculty of Chemistry, University Complutense of Madrid, 28040 Madrid, SpainDepartment of Analytical Chemistry, Faculty of Chemistry, University Complutense of Madrid, 28040 Madrid, SpainDepartment of Chemistry in Pharmaceutical Sciences, Faculty of Pharmacy, University Complutense of Madrid, 28040 Madrid, SpainDepartment of Analytical Chemistry, Faculty of Chemistry, University Complutense of Madrid, 28040 Madrid, SpainDepartment of Chemistry in Pharmaceutical Sciences, Faculty of Pharmacy, University Complutense of Madrid, 28040 Madrid, SpainDepartment of Analytical Chemistry, Faculty of Chemistry, University Complutense of Madrid, 28040 Madrid, SpainDepartment of Analytical Chemistry, Faculty of Chemistry, University Complutense of Madrid, 28040 Madrid, SpainRheumatoid arthritis (RA) is a systemic chronic autoimmune inflammatory disease that is characterized by the destruction of bone and production of autoantibodies such as rheumatoid factor (RF) and anticitrullinated protein antibodies (ACPAs). The high prevalence of this disease and the need of affordable tools for its early detection led us to prepare the first electrochemical immunoplatform for the simultaneous determination of four RA biomarkers, the autoantibodies: RF, anti-peptidyl-arginine deiminase enzyme (anti-PAD4), anti-cyclic citrullinated peptide (anti-CCP), and anti-citrullinated vimentin (anti-MCV). Functionalized magnetic beads (MBs) were used to immobilize the specific antigens, and sandwich-type immunoassays were implemented for the amperometric detection of the four autoantibodies, using the horseradish peroxidase (HRP)/H<sub>2</sub>O<sub>2</sub>/hydroquinone (HQ) system. The immunoplatform was applied to the determination of the biomarkers in human serum of twenty-two patients diagnosed with RA and four healthy individuals, and the results were validated against ELISA tests and the certified values.https://www.mdpi.com/2079-6374/13/3/381rheumatoid arthritisimmunosensingelectrochemical platformmagnetic microbeadsmultiplexautoantibodies |
| spellingShingle | Esther Sánchez-Tirado Lourdes Agüí Marta Sánchez-Paniagua Araceli González-Cortés Beatriz López-Ruiz Paloma Yáñez-Sedeño José M. Pingarrón Serum Autoantibody Biomarkers for Management of Rheumatoid Arthritis Disease Biosensors rheumatoid arthritis immunosensing electrochemical platform magnetic microbeads multiplex autoantibodies |
| title | Serum Autoantibody Biomarkers for Management of Rheumatoid Arthritis Disease |
| title_full | Serum Autoantibody Biomarkers for Management of Rheumatoid Arthritis Disease |
| title_fullStr | Serum Autoantibody Biomarkers for Management of Rheumatoid Arthritis Disease |
| title_full_unstemmed | Serum Autoantibody Biomarkers for Management of Rheumatoid Arthritis Disease |
| title_short | Serum Autoantibody Biomarkers for Management of Rheumatoid Arthritis Disease |
| title_sort | serum autoantibody biomarkers for management of rheumatoid arthritis disease |
| topic | rheumatoid arthritis immunosensing electrochemical platform magnetic microbeads multiplex autoantibodies |
| url | https://www.mdpi.com/2079-6374/13/3/381 |
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