Absence of Intraocular Lymphatic Vessels in Uveal Melanomas with Extrascleral Growth
The aim of this study was to investigate the presence of intraocular lymphatic vessels in patients with uveal melanomas and extrascleral extension using a panel of lymphatic markers. The following immunohistochemical markers were analyzed: lymphatic vessel endothelial hyaluronic acid receptor-1 (LYV...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-02-01
|
| Series: | Cancers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6694/11/2/228 |
| _version_ | 1797705890879504384 |
|---|---|
| author | Jackelien G. M. van Beek Quincy C. C. van den Bosch Nicole Naus Dion Paridaens Annelies de Klein Emine Kiliç Robert M. Verdijk |
| author_facet | Jackelien G. M. van Beek Quincy C. C. van den Bosch Nicole Naus Dion Paridaens Annelies de Klein Emine Kiliç Robert M. Verdijk |
| author_sort | Jackelien G. M. van Beek |
| collection | DOAJ |
| description | The aim of this study was to investigate the presence of intraocular lymphatic vessels in patients with uveal melanomas and extrascleral extension using a panel of lymphatic markers. The following immunohistochemical markers were analyzed: lymphatic vessel endothelial hyaluronic acid receptor-1 (LYVE-1), podoplanin (D2-40), prospero-related homeobox gene-1 (Prox-1), pan-endothelial marker cluster of differentiation 31 (CD31), and blood vessel endothelium-specific CD34. Lymphatic vessels were defined as a combination of staining of the following positive markers: LYVE-1, D2-40, Prox-1, and CD31; and no staining of the negative marker CD34. In total, 456 patients were enucleated; 16 of the 46 uveal melanomas with extrascleral extension were contained in stored paraffin tissue. Two samples of the 16 uveal melanomas showed focal positive intraocular vascular staining for LYVE-1 and co-expression of CD31 and CD34. Due to the lack of Prox-1 and D2-40, and positive expression of CD34, these cannot be classified as lymphatic vessels. In one case recruitment of an extraocular, intratumoral lymphatic vascular structure was observed in the periphery of the subconjunctival extrascleral extension. Intraocular lymphatic vessels are absent in uveal melanomas with extrascleral extension; however, we provide proof for recruitment of intratumoral lymphatics by uveal melanomas with extraocular extension from subconjunctival lymphatics that may explain the rare cases of regional lymphatic spread. A panel of antibodies is necessary to detect lymphatic vessels with high specificity. |
| first_indexed | 2024-03-12T05:44:01Z |
| format | Article |
| id | doaj.art-f82247d3a75e4740a8a4ef06f884b933 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-12T05:44:01Z |
| publishDate | 2019-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-f82247d3a75e4740a8a4ef06f884b9332023-09-03T05:49:56ZengMDPI AGCancers2072-66942019-02-0111222810.3390/cancers11020228cancers11020228Absence of Intraocular Lymphatic Vessels in Uveal Melanomas with Extrascleral GrowthJackelien G. M. van Beek0Quincy C. C. van den Bosch1Nicole Naus2Dion Paridaens3Annelies de Klein4Emine Kiliç5Robert M. Verdijk6Department of Ophthalmology, Erasmus University Medical Center, 3015 GD Rotterdam, The NetherlandsDepartment of Pathology, Erasmus University Medical Center, 3015 GD Rotterdam, The NetherlandsDepartment of Ophthalmology, Erasmus University Medical Center, 3015 GD Rotterdam, The NetherlandsDepartment of Ophthalmology, Erasmus University Medical Center, 3015 GD Rotterdam, The NetherlandsDepartment of Clinical Genetics, Erasmus University Medical Center, 3015 GD Rotterdam, The NetherlandsDepartment of Ophthalmology, Erasmus University Medical Center, 3015 GD Rotterdam, The NetherlandsDepartment of Pathology, Erasmus University Medical Center, 3015 GD Rotterdam, The NetherlandsThe aim of this study was to investigate the presence of intraocular lymphatic vessels in patients with uveal melanomas and extrascleral extension using a panel of lymphatic markers. The following immunohistochemical markers were analyzed: lymphatic vessel endothelial hyaluronic acid receptor-1 (LYVE-1), podoplanin (D2-40), prospero-related homeobox gene-1 (Prox-1), pan-endothelial marker cluster of differentiation 31 (CD31), and blood vessel endothelium-specific CD34. Lymphatic vessels were defined as a combination of staining of the following positive markers: LYVE-1, D2-40, Prox-1, and CD31; and no staining of the negative marker CD34. In total, 456 patients were enucleated; 16 of the 46 uveal melanomas with extrascleral extension were contained in stored paraffin tissue. Two samples of the 16 uveal melanomas showed focal positive intraocular vascular staining for LYVE-1 and co-expression of CD31 and CD34. Due to the lack of Prox-1 and D2-40, and positive expression of CD34, these cannot be classified as lymphatic vessels. In one case recruitment of an extraocular, intratumoral lymphatic vascular structure was observed in the periphery of the subconjunctival extrascleral extension. Intraocular lymphatic vessels are absent in uveal melanomas with extrascleral extension; however, we provide proof for recruitment of intratumoral lymphatics by uveal melanomas with extraocular extension from subconjunctival lymphatics that may explain the rare cases of regional lymphatic spread. A panel of antibodies is necessary to detect lymphatic vessels with high specificity.https://www.mdpi.com/2072-6694/11/2/228extrascleral extensionuveal melanomalymphatic vesselsLYVE-1D2-40Prox-1CD31CD34 |
| spellingShingle | Jackelien G. M. van Beek Quincy C. C. van den Bosch Nicole Naus Dion Paridaens Annelies de Klein Emine Kiliç Robert M. Verdijk Absence of Intraocular Lymphatic Vessels in Uveal Melanomas with Extrascleral Growth Cancers extrascleral extension uveal melanoma lymphatic vessels LYVE-1 D2-40 Prox-1 CD31 CD34 |
| title | Absence of Intraocular Lymphatic Vessels in Uveal Melanomas with Extrascleral Growth |
| title_full | Absence of Intraocular Lymphatic Vessels in Uveal Melanomas with Extrascleral Growth |
| title_fullStr | Absence of Intraocular Lymphatic Vessels in Uveal Melanomas with Extrascleral Growth |
| title_full_unstemmed | Absence of Intraocular Lymphatic Vessels in Uveal Melanomas with Extrascleral Growth |
| title_short | Absence of Intraocular Lymphatic Vessels in Uveal Melanomas with Extrascleral Growth |
| title_sort | absence of intraocular lymphatic vessels in uveal melanomas with extrascleral growth |
| topic | extrascleral extension uveal melanoma lymphatic vessels LYVE-1 D2-40 Prox-1 CD31 CD34 |
| url | https://www.mdpi.com/2072-6694/11/2/228 |
| work_keys_str_mv | AT jackeliengmvanbeek absenceofintraocularlymphaticvesselsinuvealmelanomaswithextrascleralgrowth AT quincyccvandenbosch absenceofintraocularlymphaticvesselsinuvealmelanomaswithextrascleralgrowth AT nicolenaus absenceofintraocularlymphaticvesselsinuvealmelanomaswithextrascleralgrowth AT dionparidaens absenceofintraocularlymphaticvesselsinuvealmelanomaswithextrascleralgrowth AT anneliesdeklein absenceofintraocularlymphaticvesselsinuvealmelanomaswithextrascleralgrowth AT eminekilic absenceofintraocularlymphaticvesselsinuvealmelanomaswithextrascleralgrowth AT robertmverdijk absenceofintraocularlymphaticvesselsinuvealmelanomaswithextrascleralgrowth |