Prospecting Biomarkers for Diagnostic and Therapeutic Approaches in Pythiosis
Pythiosis, whose etiological agent is the oomycete <i>Pythium insidiosum</i>, is a life-threatening disease that occurs mainly in tropical and subtropical countries, affecting several animal species. It is frequently found in horses in Brazil and humans in Thailand. The disease is diffic...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/7/6/423 |
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| author | Jéssica Luana Chechi Tiwa Rotchanapreeda Giselle Souza da Paz Ana Carolina Prado Alana Lucena Oliveira José Cavalcante Souza Vieira Marília Afonso Rabelo Buzalaf Anderson Messias Rodrigues Lucilene Delazari dos Santos Theerapong Krajaejun Sandra de Moraes Gimenes Bosco |
| author_facet | Jéssica Luana Chechi Tiwa Rotchanapreeda Giselle Souza da Paz Ana Carolina Prado Alana Lucena Oliveira José Cavalcante Souza Vieira Marília Afonso Rabelo Buzalaf Anderson Messias Rodrigues Lucilene Delazari dos Santos Theerapong Krajaejun Sandra de Moraes Gimenes Bosco |
| author_sort | Jéssica Luana Chechi |
| collection | DOAJ |
| description | Pythiosis, whose etiological agent is the oomycete <i>Pythium insidiosum</i>, is a life-threatening disease that occurs mainly in tropical and subtropical countries, affecting several animal species. It is frequently found in horses in Brazil and humans in Thailand. The disease is difficult to diagnose because the pathogen’s hyphae are often misdiagnosed as mucoromycete fungi in histological sections. Additionally, there is no specific antigen to use for rapid diagnosis, the availability of which could improve the prognosis in different animal species. In this scenario, we investigated which <i>P. insidiosum</i> antigens are recognized by circulating antibodies in horses and humans with pythiosis from Brazil and Thailand, respectively, using 2D immunoblotting followed by mass spectrometry for the identification of antigens. We identified 23 protein spots, 14 recognized by pooled serum from horses and humans. Seven antigens were commonly recognized by both species, such as the heat-shock cognate 70 KDa protein, the heat-shock 70 KDa protein, glucan 1,3-beta-glucosidase, fructose-bisphosphate aldolase, serine/threonine-protein phosphatase, aconitate hydratase, and 14-3-3 protein epsilon. These results demonstrate that there are common antigens recognized by the immune responses of horses and humans, and these antigens may be studied as biomarkers for improving diagnosis and treatment. |
| first_indexed | 2024-03-10T10:58:05Z |
| format | Article |
| id | doaj.art-f825a83efe5b4f588ea8e47b94d5da49 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T10:58:05Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-f825a83efe5b4f588ea8e47b94d5da492023-11-21T21:44:59ZengMDPI AGJournal of Fungi2309-608X2021-05-017642310.3390/jof7060423Prospecting Biomarkers for Diagnostic and Therapeutic Approaches in PythiosisJéssica Luana Chechi0Tiwa Rotchanapreeda1Giselle Souza da Paz2Ana Carolina Prado3Alana Lucena Oliveira4José Cavalcante Souza Vieira5Marília Afonso Rabelo Buzalaf6Anderson Messias Rodrigues7Lucilene Delazari dos Santos8Theerapong Krajaejun9Sandra de Moraes Gimenes Bosco10Department of Chemical and Biological Sciences, Institute of Biosciences, São Paulo State University (UNESP), Botucatu 18618-689, BrazilDepartment of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandFaculty of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP), Botucatu 18618-681, BrazilDepartment of Chemical and Biological Sciences, Institute of Biosciences, São Paulo State University (UNESP), Botucatu 18618-689, BrazilFaculty of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP), Botucatu 18618-681, BrazilDepartment of Chemical and Biological Sciences, Institute of Biosciences, São Paulo State University (UNESP), Botucatu 18618-689, BrazilDepartment of Biological Sciences, Bauru School of Dentistry, University of São Paulo (USP), Bauru 17012-901, BrazilDepartment of Microbiology, Immunology and Parasitology, Cell Biology Division, Federal University of São Paulo (UNIFESP), São Paulo 04023-062, BrazilCenter for the Study of Venoms and Venomous Animals (CEVAP), São Paulo State University (UNESP), Botucatu 18610-307, BrazilDepartment of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandDepartment of Chemical and Biological Sciences, Institute of Biosciences, São Paulo State University (UNESP), Botucatu 18618-689, BrazilPythiosis, whose etiological agent is the oomycete <i>Pythium insidiosum</i>, is a life-threatening disease that occurs mainly in tropical and subtropical countries, affecting several animal species. It is frequently found in horses in Brazil and humans in Thailand. The disease is difficult to diagnose because the pathogen’s hyphae are often misdiagnosed as mucoromycete fungi in histological sections. Additionally, there is no specific antigen to use for rapid diagnosis, the availability of which could improve the prognosis in different animal species. In this scenario, we investigated which <i>P. insidiosum</i> antigens are recognized by circulating antibodies in horses and humans with pythiosis from Brazil and Thailand, respectively, using 2D immunoblotting followed by mass spectrometry for the identification of antigens. We identified 23 protein spots, 14 recognized by pooled serum from horses and humans. Seven antigens were commonly recognized by both species, such as the heat-shock cognate 70 KDa protein, the heat-shock 70 KDa protein, glucan 1,3-beta-glucosidase, fructose-bisphosphate aldolase, serine/threonine-protein phosphatase, aconitate hydratase, and 14-3-3 protein epsilon. These results demonstrate that there are common antigens recognized by the immune responses of horses and humans, and these antigens may be studied as biomarkers for improving diagnosis and treatment.https://www.mdpi.com/2309-608X/7/6/423<i>Pythium insidiosum</i>pythiosisantigensdiagnosistherapyimmunoproteomics |
| spellingShingle | Jéssica Luana Chechi Tiwa Rotchanapreeda Giselle Souza da Paz Ana Carolina Prado Alana Lucena Oliveira José Cavalcante Souza Vieira Marília Afonso Rabelo Buzalaf Anderson Messias Rodrigues Lucilene Delazari dos Santos Theerapong Krajaejun Sandra de Moraes Gimenes Bosco Prospecting Biomarkers for Diagnostic and Therapeutic Approaches in Pythiosis Journal of Fungi <i>Pythium insidiosum</i> pythiosis antigens diagnosis therapy immunoproteomics |
| title | Prospecting Biomarkers for Diagnostic and Therapeutic Approaches in Pythiosis |
| title_full | Prospecting Biomarkers for Diagnostic and Therapeutic Approaches in Pythiosis |
| title_fullStr | Prospecting Biomarkers for Diagnostic and Therapeutic Approaches in Pythiosis |
| title_full_unstemmed | Prospecting Biomarkers for Diagnostic and Therapeutic Approaches in Pythiosis |
| title_short | Prospecting Biomarkers for Diagnostic and Therapeutic Approaches in Pythiosis |
| title_sort | prospecting biomarkers for diagnostic and therapeutic approaches in pythiosis |
| topic | <i>Pythium insidiosum</i> pythiosis antigens diagnosis therapy immunoproteomics |
| url | https://www.mdpi.com/2309-608X/7/6/423 |
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