Quantitative analysis of myofiber type composition in human and mouse skeletal muscles
Summary: Skeletal muscles are composed of different myofiber types characterized by the expression of myosin heavy chain isoforms, which can be affected by physical activity, aging, and pathological conditions. Here, we present a step-by-step high-throughput semi-automated approach for performing my...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723000333 |
| _version_ | 1797938784087572480 |
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| author | Tooba Abbassi-Daloii Salma el Abdellaoui Hermien E. Kan Erik van den Akker Peter A.C. ’t Hoen Vered Raz Lenard M. Voortman |
| author_facet | Tooba Abbassi-Daloii Salma el Abdellaoui Hermien E. Kan Erik van den Akker Peter A.C. ’t Hoen Vered Raz Lenard M. Voortman |
| author_sort | Tooba Abbassi-Daloii |
| collection | DOAJ |
| description | Summary: Skeletal muscles are composed of different myofiber types characterized by the expression of myosin heavy chain isoforms, which can be affected by physical activity, aging, and pathological conditions. Here, we present a step-by-step high-throughput semi-automated approach for performing myofiber type quantification of entire human or mouse muscle tissue sections, including immunofluorescence staining, image acquisition, processing, and quantification.For complete details on the use and execution of this protocol, please refer to Abbassi-Daloii et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-10T19:05:07Z |
| format | Article |
| id | doaj.art-f82b00c9fe174a4f9ce85f611d9cf880 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-10T19:05:07Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-f82b00c9fe174a4f9ce85f611d9cf8802023-01-31T04:09:32ZengElsevierSTAR Protocols2666-16672023-03-0141102075Quantitative analysis of myofiber type composition in human and mouse skeletal musclesTooba Abbassi-Daloii0Salma el Abdellaoui1Hermien E. Kan2Erik van den Akker3Peter A.C. ’t Hoen4Vered Raz5Lenard M. Voortman6Human Genetics, Leiden University Medical Center, 2333 ZA Leiden, the NetherlandsHuman Genetics, Leiden University Medical Center, 2333 ZA Leiden, the NetherlandsC.J. Gorter MRI Center, Department of Radiology, Leiden University Medical Center, 2333 ZA Leiden, the Netherlands; Duchenne Center Netherlands, the NetherlandsMolecular Epidemiology, Leiden University Medical Center, 2333 ZC Leiden, the Netherlands; Leiden Computational Biology Center, Leiden University Medical Center, 2333 ZC Leiden, the Netherlands; Delft Bioinformatics Lab, TU Delft, 2628 XE Delft, the NetherlandsHuman Genetics, Leiden University Medical Center, 2333 ZA Leiden, the Netherlands; Centre for Molecular and Biomolecular Informatics, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, 6525 GA Nijmegen, the NetherlandsHuman Genetics, Leiden University Medical Center, 2333 ZA Leiden, the Netherlands; Corresponding authorCell and Chemical Biology, Leiden University Medical Center, 2333 ZA Leiden, the Netherlands; Corresponding authorSummary: Skeletal muscles are composed of different myofiber types characterized by the expression of myosin heavy chain isoforms, which can be affected by physical activity, aging, and pathological conditions. Here, we present a step-by-step high-throughput semi-automated approach for performing myofiber type quantification of entire human or mouse muscle tissue sections, including immunofluorescence staining, image acquisition, processing, and quantification.For complete details on the use and execution of this protocol, please refer to Abbassi-Daloii et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723000333High-Throughput ScreeningMicroscopy |
| spellingShingle | Tooba Abbassi-Daloii Salma el Abdellaoui Hermien E. Kan Erik van den Akker Peter A.C. ’t Hoen Vered Raz Lenard M. Voortman Quantitative analysis of myofiber type composition in human and mouse skeletal muscles STAR Protocols High-Throughput Screening Microscopy |
| title | Quantitative analysis of myofiber type composition in human and mouse skeletal muscles |
| title_full | Quantitative analysis of myofiber type composition in human and mouse skeletal muscles |
| title_fullStr | Quantitative analysis of myofiber type composition in human and mouse skeletal muscles |
| title_full_unstemmed | Quantitative analysis of myofiber type composition in human and mouse skeletal muscles |
| title_short | Quantitative analysis of myofiber type composition in human and mouse skeletal muscles |
| title_sort | quantitative analysis of myofiber type composition in human and mouse skeletal muscles |
| topic | High-Throughput Screening Microscopy |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723000333 |
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