Fatty acid remodeling in cellular glycerophospholipids following the activation of human T cells[S]
Changes in fatty acid (FA) and glycerophospholipid (GPL) metabolism associated with cell cycle entry are not fully understood. In this study FA-GPL remodeling was investigated in resting and proliferating primary human T cells. Significant changes were measured in the composition and distribution of...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2013-10-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520352937 |
| _version_ | 1818740442310639616 |
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| author | Philippe Pierre Robichaud Katherine Boulay Jean éric Munganyiki Marc E. Surette |
| author_facet | Philippe Pierre Robichaud Katherine Boulay Jean éric Munganyiki Marc E. Surette |
| author_sort | Philippe Pierre Robichaud |
| collection | DOAJ |
| description | Changes in fatty acid (FA) and glycerophospholipid (GPL) metabolism associated with cell cycle entry are not fully understood. In this study FA-GPL remodeling was investigated in resting and proliferating primary human T cells. Significant changes were measured in the composition and distribution of FAs in GPLs following receptor activation of human T cells. The FA distribution of proliferating T cells was very similar to that of the human Jurkat T cell line and when the stimulus was removed from proliferating T cells, they stopped proliferating and the FA distribution largely reverted back to that of resting T cells. The cellular content of saturated and monounsaturated FAs was significantly increased in proliferating cells, which was associated with an induction of FA synthase and stearoyl-CoA desaturase-1 gene expression. Additionally, cellular arachidonate was redistributed in GPLs in a distinct pattern that was unlike any other FAs. This redistribution was associated with an induction of CoA-dependent and CoA-independent remodeling. Accordingly, significant changes in the expression of several acyl-CoA synthetases, lysophospholipid acyltransferases, and phospholipase A2 were measured. Overall, these results suggest that metabolic pathways are activated in proliferating T cells that may represent fundamental changes associated with human cell proliferation. |
| first_indexed | 2024-12-18T01:40:48Z |
| format | Article |
| id | doaj.art-f82bc457d0684255bbbd0797bad8a185 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-18T01:40:48Z |
| publishDate | 2013-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-f82bc457d0684255bbbd0797bad8a1852022-12-21T21:25:21ZengElsevierJournal of Lipid Research0022-22752013-10-01541026652677Fatty acid remodeling in cellular glycerophospholipids following the activation of human T cells[S]Philippe Pierre Robichaud0Katherine Boulay1Jean éric Munganyiki2Marc E. Surette3Department of Chemistry and Biochemistry, Université de Moncton, Moncton, NB E1A 3E9, Canada; and; Department of Microbiology-Infectiology and Immunology, Université Laval, Québec, QC G1V 4G2, CanadaDepartment of Chemistry and Biochemistry, Université de Moncton, Moncton, NB E1A 3E9, Canada; andDepartment of Chemistry and Biochemistry, Université de Moncton, Moncton, NB E1A 3E9, Canada; andTo whom correspondence should be addressed; Department of Chemistry and Biochemistry, Université de Moncton, Moncton, NB E1A 3E9, Canada; and; To whom correspondence should be addressedChanges in fatty acid (FA) and glycerophospholipid (GPL) metabolism associated with cell cycle entry are not fully understood. In this study FA-GPL remodeling was investigated in resting and proliferating primary human T cells. Significant changes were measured in the composition and distribution of FAs in GPLs following receptor activation of human T cells. The FA distribution of proliferating T cells was very similar to that of the human Jurkat T cell line and when the stimulus was removed from proliferating T cells, they stopped proliferating and the FA distribution largely reverted back to that of resting T cells. The cellular content of saturated and monounsaturated FAs was significantly increased in proliferating cells, which was associated with an induction of FA synthase and stearoyl-CoA desaturase-1 gene expression. Additionally, cellular arachidonate was redistributed in GPLs in a distinct pattern that was unlike any other FAs. This redistribution was associated with an induction of CoA-dependent and CoA-independent remodeling. Accordingly, significant changes in the expression of several acyl-CoA synthetases, lysophospholipid acyltransferases, and phospholipase A2 were measured. Overall, these results suggest that metabolic pathways are activated in proliferating T cells that may represent fundamental changes associated with human cell proliferation.http://www.sciencedirect.com/science/article/pii/S0022227520352937fatty acid synthasestearoyl-CoA desaturase 1acyl-CoA synthetaselysophospholipid acyltransferasephospholipase A2arachidonic acid |
| spellingShingle | Philippe Pierre Robichaud Katherine Boulay Jean éric Munganyiki Marc E. Surette Fatty acid remodeling in cellular glycerophospholipids following the activation of human T cells[S] Journal of Lipid Research fatty acid synthase stearoyl-CoA desaturase 1 acyl-CoA synthetase lysophospholipid acyltransferase phospholipase A2 arachidonic acid |
| title | Fatty acid remodeling in cellular glycerophospholipids following the activation of human T cells[S] |
| title_full | Fatty acid remodeling in cellular glycerophospholipids following the activation of human T cells[S] |
| title_fullStr | Fatty acid remodeling in cellular glycerophospholipids following the activation of human T cells[S] |
| title_full_unstemmed | Fatty acid remodeling in cellular glycerophospholipids following the activation of human T cells[S] |
| title_short | Fatty acid remodeling in cellular glycerophospholipids following the activation of human T cells[S] |
| title_sort | fatty acid remodeling in cellular glycerophospholipids following the activation of human t cells s |
| topic | fatty acid synthase stearoyl-CoA desaturase 1 acyl-CoA synthetase lysophospholipid acyltransferase phospholipase A2 arachidonic acid |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520352937 |
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