Regulatory network of miRNA, lncRNA, transcription factor and target immune response genes in bovine mastitis
Abstract Pre- and post-transcriptional modifications of gene expression are emerging as foci of disease studies, with some studies revealing the importance of non-coding transcripts, like long non-coding RNAs (lncRNAs) and microRNAs (miRNAs). We hypothesize that transcription factors (TFs), lncRNAs...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-01280-9 |
| _version_ | 1831548437215051776 |
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| author | Ashley R. Tucker Nicole A. Salazar Adeola O. Ayoola Erdoğan Memili Bolaji N. Thomas Olanrewaju B. Morenikeji |
| author_facet | Ashley R. Tucker Nicole A. Salazar Adeola O. Ayoola Erdoğan Memili Bolaji N. Thomas Olanrewaju B. Morenikeji |
| author_sort | Ashley R. Tucker |
| collection | DOAJ |
| description | Abstract Pre- and post-transcriptional modifications of gene expression are emerging as foci of disease studies, with some studies revealing the importance of non-coding transcripts, like long non-coding RNAs (lncRNAs) and microRNAs (miRNAs). We hypothesize that transcription factors (TFs), lncRNAs and miRNAs modulate immune response in bovine mastitis and could potentially serve as disease biomarkers and/or drug targets. With computational analyses, we identified candidate genes potentially regulated by miRNAs and lncRNAs base pair complementation and thermodynamic stability of binding regions. Remarkably, we found six miRNAs, two being bta-miR-223 and bta-miR-24-3p, to bind to several targets. LncRNAs NONBTAT027932.1 and XR_003029725.1, were identified to target several genes. Functional and pathway analyses revealed lipopolysaccharide-mediated signaling pathway, regulation of chemokine (C-X-C motif) ligand 2 production and regulation of IL-23 production among others. The overarching interactome deserves further in vitro/in vivo explication for specific molecular regulatory mechanisms during bovine mastitis immune response and could lay the foundation for development of disease markers and therapeutic intervention. |
| first_indexed | 2024-12-17T02:18:51Z |
| format | Article |
| id | doaj.art-f82c8b63dbce47d7a7b3290a079026c8 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-17T02:18:51Z |
| publishDate | 2021-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-f82c8b63dbce47d7a7b3290a079026c82022-12-21T22:07:19ZengNature PortfolioScientific Reports2045-23222021-11-0111111810.1038/s41598-021-01280-9Regulatory network of miRNA, lncRNA, transcription factor and target immune response genes in bovine mastitisAshley R. Tucker0Nicole A. Salazar1Adeola O. Ayoola2Erdoğan Memili3Bolaji N. Thomas4Olanrewaju B. Morenikeji5Department of Biomedical Sciences, Rochester Institute of TechnologyDepartment of Biomedical Sciences, Rochester Institute of TechnologyKunming Institute of Zoology, Chinese Academy of SciencesDepartment of Animal and Dairy Sciences, Mississippi State UniversityDepartment of Biomedical Sciences, Rochester Institute of TechnologyDivision of Biological and Health Sciences, University of Pittsburgh at BradfordAbstract Pre- and post-transcriptional modifications of gene expression are emerging as foci of disease studies, with some studies revealing the importance of non-coding transcripts, like long non-coding RNAs (lncRNAs) and microRNAs (miRNAs). We hypothesize that transcription factors (TFs), lncRNAs and miRNAs modulate immune response in bovine mastitis and could potentially serve as disease biomarkers and/or drug targets. With computational analyses, we identified candidate genes potentially regulated by miRNAs and lncRNAs base pair complementation and thermodynamic stability of binding regions. Remarkably, we found six miRNAs, two being bta-miR-223 and bta-miR-24-3p, to bind to several targets. LncRNAs NONBTAT027932.1 and XR_003029725.1, were identified to target several genes. Functional and pathway analyses revealed lipopolysaccharide-mediated signaling pathway, regulation of chemokine (C-X-C motif) ligand 2 production and regulation of IL-23 production among others. The overarching interactome deserves further in vitro/in vivo explication for specific molecular regulatory mechanisms during bovine mastitis immune response and could lay the foundation for development of disease markers and therapeutic intervention.https://doi.org/10.1038/s41598-021-01280-9 |
| spellingShingle | Ashley R. Tucker Nicole A. Salazar Adeola O. Ayoola Erdoğan Memili Bolaji N. Thomas Olanrewaju B. Morenikeji Regulatory network of miRNA, lncRNA, transcription factor and target immune response genes in bovine mastitis Scientific Reports |
| title | Regulatory network of miRNA, lncRNA, transcription factor and target immune response genes in bovine mastitis |
| title_full | Regulatory network of miRNA, lncRNA, transcription factor and target immune response genes in bovine mastitis |
| title_fullStr | Regulatory network of miRNA, lncRNA, transcription factor and target immune response genes in bovine mastitis |
| title_full_unstemmed | Regulatory network of miRNA, lncRNA, transcription factor and target immune response genes in bovine mastitis |
| title_short | Regulatory network of miRNA, lncRNA, transcription factor and target immune response genes in bovine mastitis |
| title_sort | regulatory network of mirna lncrna transcription factor and target immune response genes in bovine mastitis |
| url | https://doi.org/10.1038/s41598-021-01280-9 |
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