The Protective Effects of Bushen Daozhuo Granule on Chronic Non-bacterial Prostatitis
Background: Chronic non-bacterial prostatitis (CNP), one of the most common chronic diseases in urology, leads to pain in the prostate and dysuria, critically affecting the physical or mental health of patients. However, there are no standard treatment approaches for the treatment of CNP in the clin...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-01-01
|
| Series: | Frontiers in Pharmacology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2023.1281002/full |
| _version_ | 1797366239362809856 |
|---|---|
| author | Dalin Sun Dong Xing Dandan Wang Yuanyuan Liu Bin Cai Weimin Deng Qinglin Hu Wenjun Ma Baofang Jin |
| author_facet | Dalin Sun Dong Xing Dandan Wang Yuanyuan Liu Bin Cai Weimin Deng Qinglin Hu Wenjun Ma Baofang Jin |
| author_sort | Dalin Sun |
| collection | DOAJ |
| description | Background: Chronic non-bacterial prostatitis (CNP), one of the most common chronic diseases in urology, leads to pain in the prostate and dysuria, critically affecting the physical or mental health of patients. However, there are no standard treatment approaches for the treatment of CNP in the clinic. Although the clinical application of Bushen Daozhuo granule (BSDZG) offers hope to CNP patients in China, the mechanisms of BSDZG in treating CNP are still not entirely clear. Hence, we aimed to investigate the novel therapeutic mechanisms of BSDZG on CNP.Methods: In this study, we first assayed the prostate index of rats and then determined the anti-inflammatory and anti-apoptotic effects of BSDZG on CNP in vivo and in vitro by employing ELISA kits and TUNEL staining. Next, we investigated whether the anti-inflammatory and anti-apoptotic mechanisms of BSDZG on prostate protein-induced rats and lipopolysaccharide (LPS) induced RWPE-1 cells were related to the AKT, p38 MAPK, and NF-κB pathways with the help of Western blot. Finally, the influence of BSDZG on the interaction between the p38 MAPK and NF-κB pathway in LPS-induced RWPE-1 cells was explored by adopting dehydrocorydaline (DHC, p38 MAPK activator) with the help of ELISA kits and Western blot.Results:In vivo, BSDZG effectively reduced the prostate index. In vivo and in vitro, BSDZG dramatically declined the level of two pro-inflammatory cytokines, TNF-α and IL-1β, as well as the apoptosis rate. Moreover, in vivo and in vitro, BSDZG memorably upregulated the expression level of p-AKT, and substantially downregulated the expression level of p-p38 MAPK and NF-κB2. The activation of p38 MAPK significantly reversed the moderation effects of BSDZG on the level of TNF-α and IL-1β, as well as the expression level of p-p38 MAPK and NF-κB2 in vitro.Conclusion: To sum up, the in vivo and in vitro therapeutic mechanisms of BSDZG on CNP were reflected as the anti-inflammation and anti-apoptosis that was formed by inhibiting the level of pro-inflammatory cytokines, TNF-α and IL-1β, to regulate the AKT, p38 MAPK, and NF-κB pathways, and the anti-inflammatory effect of BSDZG was realized by suppressing the p38 MAPK pathway to inhibit the downstream NF-κB pathway. |
| first_indexed | 2024-03-08T17:02:32Z |
| format | Article |
| id | doaj.art-f82d0b6b467f417eb260e1e586aaf1be |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-03-08T17:02:32Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-f82d0b6b467f417eb260e1e586aaf1be2024-01-04T10:09:27ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122024-01-011410.3389/fphar.2023.12810021281002The Protective Effects of Bushen Daozhuo Granule on Chronic Non-bacterial ProstatitisDalin Sun0Dong Xing1Dandan Wang2Yuanyuan Liu3Bin Cai4Weimin Deng5Qinglin Hu6Wenjun Ma7Baofang Jin8Andrology Department of Integrative Medicine, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, ChinaSchool of Medicine, Southeast University, Nanjing, ChinaSchool of Medicine, Southeast University, Nanjing, ChinaSchool of Medicine, Southeast University, Nanjing, ChinaAndrology Department of Integrative Medicine, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, ChinaAndrology Department of Integrative Medicine, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, ChinaDepartment of Urology, Chuzhou Integrated Hospital of Chinese and Western Medicine, Affiliated to Anhui University of Traditional Chinese Medicine, Chuzhou, ChinaDepartment of Urology, Chuzhou Integrated Hospital of Chinese and Western Medicine, Affiliated to Anhui University of Traditional Chinese Medicine, Chuzhou, ChinaAndrology Department of Integrative Medicine, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, ChinaBackground: Chronic non-bacterial prostatitis (CNP), one of the most common chronic diseases in urology, leads to pain in the prostate and dysuria, critically affecting the physical or mental health of patients. However, there are no standard treatment approaches for the treatment of CNP in the clinic. Although the clinical application of Bushen Daozhuo granule (BSDZG) offers hope to CNP patients in China, the mechanisms of BSDZG in treating CNP are still not entirely clear. Hence, we aimed to investigate the novel therapeutic mechanisms of BSDZG on CNP.Methods: In this study, we first assayed the prostate index of rats and then determined the anti-inflammatory and anti-apoptotic effects of BSDZG on CNP in vivo and in vitro by employing ELISA kits and TUNEL staining. Next, we investigated whether the anti-inflammatory and anti-apoptotic mechanisms of BSDZG on prostate protein-induced rats and lipopolysaccharide (LPS) induced RWPE-1 cells were related to the AKT, p38 MAPK, and NF-κB pathways with the help of Western blot. Finally, the influence of BSDZG on the interaction between the p38 MAPK and NF-κB pathway in LPS-induced RWPE-1 cells was explored by adopting dehydrocorydaline (DHC, p38 MAPK activator) with the help of ELISA kits and Western blot.Results:In vivo, BSDZG effectively reduced the prostate index. In vivo and in vitro, BSDZG dramatically declined the level of two pro-inflammatory cytokines, TNF-α and IL-1β, as well as the apoptosis rate. Moreover, in vivo and in vitro, BSDZG memorably upregulated the expression level of p-AKT, and substantially downregulated the expression level of p-p38 MAPK and NF-κB2. The activation of p38 MAPK significantly reversed the moderation effects of BSDZG on the level of TNF-α and IL-1β, as well as the expression level of p-p38 MAPK and NF-κB2 in vitro.Conclusion: To sum up, the in vivo and in vitro therapeutic mechanisms of BSDZG on CNP were reflected as the anti-inflammation and anti-apoptosis that was formed by inhibiting the level of pro-inflammatory cytokines, TNF-α and IL-1β, to regulate the AKT, p38 MAPK, and NF-κB pathways, and the anti-inflammatory effect of BSDZG was realized by suppressing the p38 MAPK pathway to inhibit the downstream NF-κB pathway.https://www.frontiersin.org/articles/10.3389/fphar.2023.1281002/fullBushen Daozhuo granule (BSDZG)chronic non-bacterial prostatitis (CNP)anti-inflammationanti-apoptosisTNF-α |
| spellingShingle | Dalin Sun Dong Xing Dandan Wang Yuanyuan Liu Bin Cai Weimin Deng Qinglin Hu Wenjun Ma Baofang Jin The Protective Effects of Bushen Daozhuo Granule on Chronic Non-bacterial Prostatitis Frontiers in Pharmacology Bushen Daozhuo granule (BSDZG) chronic non-bacterial prostatitis (CNP) anti-inflammation anti-apoptosis TNF-α |
| title | The Protective Effects of Bushen Daozhuo Granule on Chronic Non-bacterial Prostatitis |
| title_full | The Protective Effects of Bushen Daozhuo Granule on Chronic Non-bacterial Prostatitis |
| title_fullStr | The Protective Effects of Bushen Daozhuo Granule on Chronic Non-bacterial Prostatitis |
| title_full_unstemmed | The Protective Effects of Bushen Daozhuo Granule on Chronic Non-bacterial Prostatitis |
| title_short | The Protective Effects of Bushen Daozhuo Granule on Chronic Non-bacterial Prostatitis |
| title_sort | protective effects of bushen daozhuo granule on chronic non bacterial prostatitis |
| topic | Bushen Daozhuo granule (BSDZG) chronic non-bacterial prostatitis (CNP) anti-inflammation anti-apoptosis TNF-α |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2023.1281002/full |
| work_keys_str_mv | AT dalinsun theprotectiveeffectsofbushendaozhuogranuleonchronicnonbacterialprostatitis AT dongxing theprotectiveeffectsofbushendaozhuogranuleonchronicnonbacterialprostatitis AT dandanwang theprotectiveeffectsofbushendaozhuogranuleonchronicnonbacterialprostatitis AT yuanyuanliu theprotectiveeffectsofbushendaozhuogranuleonchronicnonbacterialprostatitis AT bincai theprotectiveeffectsofbushendaozhuogranuleonchronicnonbacterialprostatitis AT weimindeng theprotectiveeffectsofbushendaozhuogranuleonchronicnonbacterialprostatitis AT qinglinhu theprotectiveeffectsofbushendaozhuogranuleonchronicnonbacterialprostatitis AT wenjunma theprotectiveeffectsofbushendaozhuogranuleonchronicnonbacterialprostatitis AT baofangjin theprotectiveeffectsofbushendaozhuogranuleonchronicnonbacterialprostatitis AT dalinsun protectiveeffectsofbushendaozhuogranuleonchronicnonbacterialprostatitis AT dongxing protectiveeffectsofbushendaozhuogranuleonchronicnonbacterialprostatitis AT dandanwang protectiveeffectsofbushendaozhuogranuleonchronicnonbacterialprostatitis AT yuanyuanliu protectiveeffectsofbushendaozhuogranuleonchronicnonbacterialprostatitis AT bincai protectiveeffectsofbushendaozhuogranuleonchronicnonbacterialprostatitis AT weimindeng protectiveeffectsofbushendaozhuogranuleonchronicnonbacterialprostatitis AT qinglinhu protectiveeffectsofbushendaozhuogranuleonchronicnonbacterialprostatitis AT wenjunma protectiveeffectsofbushendaozhuogranuleonchronicnonbacterialprostatitis AT baofangjin protectiveeffectsofbushendaozhuogranuleonchronicnonbacterialprostatitis |