Selenium nanoparticles functionalized by mushroom polysaccharide-protein complex: A novel nano-mineral for managing postmenopausal osteoporosis
With an increase in aging population, osteoporosis has become one of the major public health issues nowadays, affecting over 200 million people worldwide. Since current medications are associated with various side effects, there is a clear clinical need to develop alternative therapeutics for managi...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623004322 |
| _version_ | 1797632739109765120 |
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| author | Kar-Him Luk Cheuk-Hin Chan Zhi-Wen Liu Chun-Wei Jiao Xiao-Bing Yang Xiao-Li Dong Ka-Hing Wong |
| author_facet | Kar-Him Luk Cheuk-Hin Chan Zhi-Wen Liu Chun-Wei Jiao Xiao-Bing Yang Xiao-Li Dong Ka-Hing Wong |
| author_sort | Kar-Him Luk |
| collection | DOAJ |
| description | With an increase in aging population, osteoporosis has become one of the major public health issues nowadays, affecting over 200 million people worldwide. Since current medications are associated with various side effects, there is a clear clinical need to develop alternative therapeutics for managing osteoporosis. Selenium is an essential trace mineral, which has been proved to play important role in bone health. However, the narrow therapeutic window of selenium has greatly hindered its further development. Recently, selenium nanoparticles (SeNPs) have become a new selenium source due to its promising bioactivity and low toxicity. Nevertheless, scientific research concerning their effects on bone health is still very limited. By using the mushroom polysaccharide-protein complex isolated from Cordyceps sinensis, we have successfully prepared novel SeNPs (“Cs4-SeNPs”) with well-characterized structure and high stability. Interestingly, Cs4-SeNPs (10 μM) were found to markedly induce proliferation, differentiation, and mineralization of the pre-osteoblast murine MC3T3-E1 cells. Further mechanistic study discovered that after cellular internalization via endocytosis, Cs4-SeNPs would trigger Nox4-derived intracellular ROS generation in the MC3T3-E1 cells followed by promoting downstream BMP-2 gene transcription and activating BMP signaling via both Smad dependent and Smad independent p38 MAPK pathways. More importantly, Cs4-SeNPs (25–500 μg/kg BW/d) exhibitedpromising in vivo bone protective efficacy against OVX-induced osteoporosis by promoting bone formation, inhibiting bone resorption, and improving bone microarchitecture after oral gavage for 6 weeks. Findings of this study collectively suggested that Cs4-SeNPs is of great potential to be further developed into a safe and evidence-based nano-mineral for managing postmenopausal osteoporosis. |
| first_indexed | 2024-03-11T11:42:05Z |
| format | Article |
| id | doaj.art-f82d44160fc14647a24db6269bb9fc53 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-03-11T11:42:05Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-f82d44160fc14647a24db6269bb9fc532023-11-10T04:16:09ZengElsevierJournal of Functional Foods1756-46462023-11-01110105832Selenium nanoparticles functionalized by mushroom polysaccharide-protein complex: A novel nano-mineral for managing postmenopausal osteoporosisKar-Him Luk0Cheuk-Hin Chan1Zhi-Wen Liu2Chun-Wei Jiao3Xiao-Bing Yang4Xiao-Li Dong5Ka-Hing Wong6Research Institute for Future Food, The Hong Kong Polytechnic University, Hong Kong, China; Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hong Kong, ChinaDepartment of Food Science and Nutrition, The Hong Kong Polytechnic University, Hong Kong, ChinaResearch Institute for Future Food, The Hong Kong Polytechnic University, Hong Kong, ChinaGuangdong Yuewei Edible Fungi Technology Co., Ltd, Guangzhou, ChinaGuangdong Institute of Microbiology, Guangzhou, ChinaResearch Institute for Future Food, The Hong Kong Polytechnic University, Hong Kong, China; Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hong Kong, China; Corresponding authors at: Room Y809, 8/F, Y Core, Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China (Ka-Hing Wong). Room Y817, 8/F, Y Core, Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hung Hom, Kowloon Hong Kong, China (Xiao-Li Dong).Research Institute for Future Food, The Hong Kong Polytechnic University, Hong Kong, China; Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hong Kong, China; Corresponding authors at: Room Y809, 8/F, Y Core, Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China (Ka-Hing Wong). Room Y817, 8/F, Y Core, Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hung Hom, Kowloon Hong Kong, China (Xiao-Li Dong).With an increase in aging population, osteoporosis has become one of the major public health issues nowadays, affecting over 200 million people worldwide. Since current medications are associated with various side effects, there is a clear clinical need to develop alternative therapeutics for managing osteoporosis. Selenium is an essential trace mineral, which has been proved to play important role in bone health. However, the narrow therapeutic window of selenium has greatly hindered its further development. Recently, selenium nanoparticles (SeNPs) have become a new selenium source due to its promising bioactivity and low toxicity. Nevertheless, scientific research concerning their effects on bone health is still very limited. By using the mushroom polysaccharide-protein complex isolated from Cordyceps sinensis, we have successfully prepared novel SeNPs (“Cs4-SeNPs”) with well-characterized structure and high stability. Interestingly, Cs4-SeNPs (10 μM) were found to markedly induce proliferation, differentiation, and mineralization of the pre-osteoblast murine MC3T3-E1 cells. Further mechanistic study discovered that after cellular internalization via endocytosis, Cs4-SeNPs would trigger Nox4-derived intracellular ROS generation in the MC3T3-E1 cells followed by promoting downstream BMP-2 gene transcription and activating BMP signaling via both Smad dependent and Smad independent p38 MAPK pathways. More importantly, Cs4-SeNPs (25–500 μg/kg BW/d) exhibitedpromising in vivo bone protective efficacy against OVX-induced osteoporosis by promoting bone formation, inhibiting bone resorption, and improving bone microarchitecture after oral gavage for 6 weeks. Findings of this study collectively suggested that Cs4-SeNPs is of great potential to be further developed into a safe and evidence-based nano-mineral for managing postmenopausal osteoporosis.http://www.sciencedirect.com/science/article/pii/S1756464623004322Selenium nanoparticlesPostmenopausal osteoporosisMushroom polysaccharides-protein complexCordyceps sinensisNano-mineral |
| spellingShingle | Kar-Him Luk Cheuk-Hin Chan Zhi-Wen Liu Chun-Wei Jiao Xiao-Bing Yang Xiao-Li Dong Ka-Hing Wong Selenium nanoparticles functionalized by mushroom polysaccharide-protein complex: A novel nano-mineral for managing postmenopausal osteoporosis Journal of Functional Foods Selenium nanoparticles Postmenopausal osteoporosis Mushroom polysaccharides-protein complex Cordyceps sinensis Nano-mineral |
| title | Selenium nanoparticles functionalized by mushroom polysaccharide-protein complex: A novel nano-mineral for managing postmenopausal osteoporosis |
| title_full | Selenium nanoparticles functionalized by mushroom polysaccharide-protein complex: A novel nano-mineral for managing postmenopausal osteoporosis |
| title_fullStr | Selenium nanoparticles functionalized by mushroom polysaccharide-protein complex: A novel nano-mineral for managing postmenopausal osteoporosis |
| title_full_unstemmed | Selenium nanoparticles functionalized by mushroom polysaccharide-protein complex: A novel nano-mineral for managing postmenopausal osteoporosis |
| title_short | Selenium nanoparticles functionalized by mushroom polysaccharide-protein complex: A novel nano-mineral for managing postmenopausal osteoporosis |
| title_sort | selenium nanoparticles functionalized by mushroom polysaccharide protein complex a novel nano mineral for managing postmenopausal osteoporosis |
| topic | Selenium nanoparticles Postmenopausal osteoporosis Mushroom polysaccharides-protein complex Cordyceps sinensis Nano-mineral |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623004322 |
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