Counteracting the Ramifications of UVB Irradiation and Photoaging with <i>Swietenia macrophylla</i> King Seed
In this day and age, the expectation of cosmetic products to effectively slow down skin photoaging is constantly increasing. However, the detrimental effects of UVB on the skin are not easy to tackle as UVB dysregulates a wide range of molecular changes on the cellular level. In our research, irradi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/7/2000 |
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| author | Camille Keisha Mahendra Syafiq Asnawi Zainal Abidin Thet Thet Htar Lay-Hong Chuah Shafi Ullah Khan Long Chiau Ming Siah Ying Tang Priyia Pusparajah Bey Hing Goh |
| author_facet | Camille Keisha Mahendra Syafiq Asnawi Zainal Abidin Thet Thet Htar Lay-Hong Chuah Shafi Ullah Khan Long Chiau Ming Siah Ying Tang Priyia Pusparajah Bey Hing Goh |
| author_sort | Camille Keisha Mahendra |
| collection | DOAJ |
| description | In this day and age, the expectation of cosmetic products to effectively slow down skin photoaging is constantly increasing. However, the detrimental effects of UVB on the skin are not easy to tackle as UVB dysregulates a wide range of molecular changes on the cellular level. In our research, irradiated keratinocyte cells not only experienced a compromise in their redox system, but processes from RNA translation to protein synthesis and folding were also affected. Aside from this, proteins involved in various other processes like DNA repair and maintenance, glycolysis, cell growth, proliferation, and migration were affected while the cells approached imminent cell death. Additionally, the collagen degradation pathway was also activated by UVB irradiation through the upregulation of inflammatory and collagen degrading markers. Nevertheless, with the treatment of <i>Swietenia macrophylla</i> (<i>S. macrophylla</i>) seed extract and fractions, the dysregulation of many genes and proteins by UVB was reversed. The reversal effects were particularly promising with the <i>S. macrophylla</i> hexane fraction (SMHF) and <i>S. macrophylla</i> ethyl acetate fraction (SMEAF). SMHF was able to oppose the detrimental effects of UVB in several different processes such as the redox system, DNA repair and maintenance, RNA transcription to translation, protein maintenance and synthesis, cell growth, migration and proliferation, and cell glycolysis, while SMEAF successfully suppressed markers related to skin inflammation, collagen degradation, and cell apoptosis. Thus, in summary, our research not only provided a deeper insight into the molecular changes within irradiated keratinocytes, but also serves as a model platform for future cosmetic research to build upon. Subsequently, both SMHF and SMEAF also displayed potential photoprotective properties that warrant further fractionation and in vivo clinical trials to investigate and obtain potential novel bioactive compounds against photoaging. |
| first_indexed | 2024-03-10T12:42:06Z |
| format | Article |
| id | doaj.art-f82db505dc5f4cbbadeb9204b21722d6 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T12:42:06Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-f82db505dc5f4cbbadeb9204b21722d62023-11-21T13:49:20ZengMDPI AGMolecules1420-30492021-04-01267200010.3390/molecules26072000Counteracting the Ramifications of UVB Irradiation and Photoaging with <i>Swietenia macrophylla</i> King SeedCamille Keisha Mahendra0Syafiq Asnawi Zainal Abidin1Thet Thet Htar2Lay-Hong Chuah3Shafi Ullah Khan4Long Chiau Ming5Siah Ying Tang6Priyia Pusparajah7Bey Hing Goh8Biofunctional Molecule Exploratory Research Group, School of Pharmacy, Monash University Malaysia, Bandar Sunway 47500, MalaysiaLiquid Chromatography Mass Spectrometry (LCMS) Platform, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway 47500, MalaysiaBiofunctional Molecule Exploratory Research Group, School of Pharmacy, Monash University Malaysia, Bandar Sunway 47500, MalaysiaBiofunctional Molecule Exploratory Research Group, School of Pharmacy, Monash University Malaysia, Bandar Sunway 47500, MalaysiaBiofunctional Molecule Exploratory Research Group, School of Pharmacy, Monash University Malaysia, Bandar Sunway 47500, MalaysiaPAP Rashidah Sa’adatul Bolkiah Institute of Health Sciences, Universiti Brunei Darussalam, Gadong BE1410, BruneiChemical Engineering Discipline, School of Engineering, Monash University Malaysia, Bandar Sunway 47500, MalaysiaMedical Health and Translational Research Group, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Bandar Sunway 47500, MalaysiaBiofunctional Molecule Exploratory Research Group, School of Pharmacy, Monash University Malaysia, Bandar Sunway 47500, MalaysiaIn this day and age, the expectation of cosmetic products to effectively slow down skin photoaging is constantly increasing. However, the detrimental effects of UVB on the skin are not easy to tackle as UVB dysregulates a wide range of molecular changes on the cellular level. In our research, irradiated keratinocyte cells not only experienced a compromise in their redox system, but processes from RNA translation to protein synthesis and folding were also affected. Aside from this, proteins involved in various other processes like DNA repair and maintenance, glycolysis, cell growth, proliferation, and migration were affected while the cells approached imminent cell death. Additionally, the collagen degradation pathway was also activated by UVB irradiation through the upregulation of inflammatory and collagen degrading markers. Nevertheless, with the treatment of <i>Swietenia macrophylla</i> (<i>S. macrophylla</i>) seed extract and fractions, the dysregulation of many genes and proteins by UVB was reversed. The reversal effects were particularly promising with the <i>S. macrophylla</i> hexane fraction (SMHF) and <i>S. macrophylla</i> ethyl acetate fraction (SMEAF). SMHF was able to oppose the detrimental effects of UVB in several different processes such as the redox system, DNA repair and maintenance, RNA transcription to translation, protein maintenance and synthesis, cell growth, migration and proliferation, and cell glycolysis, while SMEAF successfully suppressed markers related to skin inflammation, collagen degradation, and cell apoptosis. Thus, in summary, our research not only provided a deeper insight into the molecular changes within irradiated keratinocytes, but also serves as a model platform for future cosmetic research to build upon. Subsequently, both SMHF and SMEAF also displayed potential photoprotective properties that warrant further fractionation and in vivo clinical trials to investigate and obtain potential novel bioactive compounds against photoaging.https://www.mdpi.com/1420-3049/26/7/2000photoagingproteomicsgenomics<i>Swietenia macrophylla</i>UV irradiationkeratinocytes |
| spellingShingle | Camille Keisha Mahendra Syafiq Asnawi Zainal Abidin Thet Thet Htar Lay-Hong Chuah Shafi Ullah Khan Long Chiau Ming Siah Ying Tang Priyia Pusparajah Bey Hing Goh Counteracting the Ramifications of UVB Irradiation and Photoaging with <i>Swietenia macrophylla</i> King Seed Molecules photoaging proteomics genomics <i>Swietenia macrophylla</i> UV irradiation keratinocytes |
| title | Counteracting the Ramifications of UVB Irradiation and Photoaging with <i>Swietenia macrophylla</i> King Seed |
| title_full | Counteracting the Ramifications of UVB Irradiation and Photoaging with <i>Swietenia macrophylla</i> King Seed |
| title_fullStr | Counteracting the Ramifications of UVB Irradiation and Photoaging with <i>Swietenia macrophylla</i> King Seed |
| title_full_unstemmed | Counteracting the Ramifications of UVB Irradiation and Photoaging with <i>Swietenia macrophylla</i> King Seed |
| title_short | Counteracting the Ramifications of UVB Irradiation and Photoaging with <i>Swietenia macrophylla</i> King Seed |
| title_sort | counteracting the ramifications of uvb irradiation and photoaging with i swietenia macrophylla i king seed |
| topic | photoaging proteomics genomics <i>Swietenia macrophylla</i> UV irradiation keratinocytes |
| url | https://www.mdpi.com/1420-3049/26/7/2000 |
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