Growth of Microorganisms in Total Parenteral Nutrition Solutions Containing Lipid
<p><b>Background: </b>To identify the microorganisms that can grow rapidly in total parenteral nutrition (TPN) solutions, we investigated the growth of the major causes of catheter-related blood stream infection (<i>Staphylococcus aureus</i>, <i>Serratia marcescen...
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| Format: | Article |
| Language: | English |
| Published: |
Ivyspring International Publisher
2010-01-01
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| Series: | International Journal of Medical Sciences |
| Online Access: | http://www.medsci.org/v07p0101.htm |
| _version_ | 1811235702652796928 |
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| author | Takashi Kuwahara, Kazuyuki Shimono, Shinya Kaneda, Takumi Tamura, Masao Ichihara, Yoshifumi Nakashima |
| author_facet | Takashi Kuwahara, Kazuyuki Shimono, Shinya Kaneda, Takumi Tamura, Masao Ichihara, Yoshifumi Nakashima |
| author_sort | Takashi Kuwahara, Kazuyuki Shimono, Shinya Kaneda, Takumi Tamura, Masao Ichihara, Yoshifumi Nakashima |
| collection | DOAJ |
| description | <p><b>Background: </b>To identify the microorganisms that can grow rapidly in total parenteral nutrition (TPN) solutions, we investigated the growth of the major causes of catheter-related blood stream infection (<i>Staphylococcus aureus</i>, <i>Serratia marcescens</i>, <i>Bacillus cereus</i>, and <i>Candida albicans</i>) in TPN solutions containing lipid. <b>Methods:</b> The pH value of a TPN solution containing lipid (pH 6.0, containing 20 ppm of NaHSO<sub>3</sub>) was adjusted by the addition of HCl to 5.7, 5.4, or 4.9. The pH value of another TPN solution (pH5.5, containing 400 ppm of NaHSO<sub>3</sub>) was adjusted by the addition of NaOH to 5.9, 6.3, or 6.8. A specific number of each microorganism was added to 10 mL of each test solution and incubated at room temperature. The number of microorganisms was counted as colony forming units at 0, 24, and 48 hrs later. <b>Results:</b> <i>C albicans</i> increased similarly at any pH values in the TPN solution. The bacterial species also increased rapidly at pH6.0 in the solution containing 20 ppm of NaHSO<sub>3</sub>, but growth was suppressed as the pH value was reduced, with growth halted at pH4.9. However, these bacterial species did not increase, even at pH5.9, in the other solution containing 400 ppm of NaHSO<sub>3</sub>. <b>Conclusions:</b> These results suggest that <i>Candida</i> species can grow rapidly in almost all TPN solutions regardless of the acidity, lipid, and NaHSO<sub>3</sub>; also, some bacterial species may grow in TPN solutions containing lipid unless the pH value is 5.0 or less. Therefore, each TPN solution should be examined whether or not the bacterial species can proliferate.</p> |
| first_indexed | 2024-04-12T11:55:26Z |
| format | Article |
| id | doaj.art-f82e37783c274190a8a430d8932fae06 |
| institution | Directory Open Access Journal |
| issn | 1449-1907 |
| language | English |
| last_indexed | 2024-04-12T11:55:26Z |
| publishDate | 2010-01-01 |
| publisher | Ivyspring International Publisher |
| record_format | Article |
| series | International Journal of Medical Sciences |
| spelling | doaj.art-f82e37783c274190a8a430d8932fae062022-12-22T03:34:01ZengIvyspring International PublisherInternational Journal of Medical Sciences1449-19072010-01-0173101109Growth of Microorganisms in Total Parenteral Nutrition Solutions Containing LipidTakashi Kuwahara, Kazuyuki Shimono, Shinya Kaneda, Takumi Tamura, Masao Ichihara, Yoshifumi Nakashima<p><b>Background: </b>To identify the microorganisms that can grow rapidly in total parenteral nutrition (TPN) solutions, we investigated the growth of the major causes of catheter-related blood stream infection (<i>Staphylococcus aureus</i>, <i>Serratia marcescens</i>, <i>Bacillus cereus</i>, and <i>Candida albicans</i>) in TPN solutions containing lipid. <b>Methods:</b> The pH value of a TPN solution containing lipid (pH 6.0, containing 20 ppm of NaHSO<sub>3</sub>) was adjusted by the addition of HCl to 5.7, 5.4, or 4.9. The pH value of another TPN solution (pH5.5, containing 400 ppm of NaHSO<sub>3</sub>) was adjusted by the addition of NaOH to 5.9, 6.3, or 6.8. A specific number of each microorganism was added to 10 mL of each test solution and incubated at room temperature. The number of microorganisms was counted as colony forming units at 0, 24, and 48 hrs later. <b>Results:</b> <i>C albicans</i> increased similarly at any pH values in the TPN solution. The bacterial species also increased rapidly at pH6.0 in the solution containing 20 ppm of NaHSO<sub>3</sub>, but growth was suppressed as the pH value was reduced, with growth halted at pH4.9. However, these bacterial species did not increase, even at pH5.9, in the other solution containing 400 ppm of NaHSO<sub>3</sub>. <b>Conclusions:</b> These results suggest that <i>Candida</i> species can grow rapidly in almost all TPN solutions regardless of the acidity, lipid, and NaHSO<sub>3</sub>; also, some bacterial species may grow in TPN solutions containing lipid unless the pH value is 5.0 or less. Therefore, each TPN solution should be examined whether or not the bacterial species can proliferate.</p>http://www.medsci.org/v07p0101.htm |
| spellingShingle | Takashi Kuwahara, Kazuyuki Shimono, Shinya Kaneda, Takumi Tamura, Masao Ichihara, Yoshifumi Nakashima Growth of Microorganisms in Total Parenteral Nutrition Solutions Containing Lipid International Journal of Medical Sciences |
| title | Growth of Microorganisms in Total Parenteral Nutrition Solutions Containing Lipid |
| title_full | Growth of Microorganisms in Total Parenteral Nutrition Solutions Containing Lipid |
| title_fullStr | Growth of Microorganisms in Total Parenteral Nutrition Solutions Containing Lipid |
| title_full_unstemmed | Growth of Microorganisms in Total Parenteral Nutrition Solutions Containing Lipid |
| title_short | Growth of Microorganisms in Total Parenteral Nutrition Solutions Containing Lipid |
| title_sort | growth of microorganisms in total parenteral nutrition solutions containing lipid |
| url | http://www.medsci.org/v07p0101.htm |
| work_keys_str_mv | AT takashikuwaharakazuyukishimonoshinyakanedatakumitamuramasaoichiharayoshifuminakashima growthofmicroorganismsintotalparenteralnutritionsolutionscontaininglipid |