<i>MtTCP18</i> Regulates Plant Structure in <i>Medicago truncatula</i>
Plant structure has a large influence on crop yield formation, with branching and plant height being the important factors that make it up. We identified a gene, <i>MtTCP18</i>, encoding a TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR (TCP) transcription factor highly conserved...
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| Format: | Article |
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MDPI AG
2024-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/13/7/1012 |
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| author | Xiaoyue Su Junzan Zheng Xiaoxuan Diao Zhongyi Yang Deyue Yu Fang Huang |
| author_facet | Xiaoyue Su Junzan Zheng Xiaoxuan Diao Zhongyi Yang Deyue Yu Fang Huang |
| author_sort | Xiaoyue Su |
| collection | DOAJ |
| description | Plant structure has a large influence on crop yield formation, with branching and plant height being the important factors that make it up. We identified a gene, <i>MtTCP18</i>, encoding a TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR (TCP) transcription factor highly conserved with <i>Arabidopsis</i> gene <i>BRC1</i> (BRANCHED1) in <i>Medicago truncatula</i>. Sequence analysis revealed that <i>MtTCP18</i> included a conserved basic helix–loop–helix (BHLH) motif and R domain. Expression analysis showed that <i>MtTCP18</i> was expressed in all organs examined, with relatively higher expression in pods and axillary buds. Subcellular localization analysis showed that MtTCP18 was localized in the nucleus and exhibited transcriptional activation activity. These results supported its role as a transcription factor. Meanwhile, we identified a homozygous mutant line (NF14875) with a mutation caused by Tnt1 insertion into <i>MtTCP18</i>. Mutant analysis showed that the mutation of <i>MtTCP18</i> altered plant structure, with increased plant height and branch number. Moreover, we found that the expression of auxin early response genes was modulated in the mutant. Therefore, <i>MtTCP18</i> may be a promising candidate gene for breeders to optimize plant structure for crop improvement. |
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| id | doaj.art-f832a46b62a64c35bf18a90eb3e22343 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-04-24T10:37:23Z |
| publishDate | 2024-04-01 |
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| series | Plants |
| spelling | doaj.art-f832a46b62a64c35bf18a90eb3e223432024-04-12T13:24:51ZengMDPI AGPlants2223-77472024-04-01137101210.3390/plants13071012<i>MtTCP18</i> Regulates Plant Structure in <i>Medicago truncatula</i>Xiaoyue Su0Junzan Zheng1Xiaoxuan Diao2Zhongyi Yang3Deyue Yu4Fang Huang5Key Laboratory of Biology and Genetics Improvement of Soybean, Ministry of Agriculture, Zhongshan Biological Breeding Laboratory (ZSBBL), National Innovation Platform for Soybean Breeding and Industry-Education Integration, State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Biology and Genetics Improvement of Soybean, Ministry of Agriculture, Zhongshan Biological Breeding Laboratory (ZSBBL), National Innovation Platform for Soybean Breeding and Industry-Education Integration, State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Biology and Genetics Improvement of Soybean, Ministry of Agriculture, Zhongshan Biological Breeding Laboratory (ZSBBL), National Innovation Platform for Soybean Breeding and Industry-Education Integration, State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Biology and Genetics Improvement of Soybean, Ministry of Agriculture, Zhongshan Biological Breeding Laboratory (ZSBBL), National Innovation Platform for Soybean Breeding and Industry-Education Integration, State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Biology and Genetics Improvement of Soybean, Ministry of Agriculture, Zhongshan Biological Breeding Laboratory (ZSBBL), National Innovation Platform for Soybean Breeding and Industry-Education Integration, State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Biology and Genetics Improvement of Soybean, Ministry of Agriculture, Zhongshan Biological Breeding Laboratory (ZSBBL), National Innovation Platform for Soybean Breeding and Industry-Education Integration, State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, ChinaPlant structure has a large influence on crop yield formation, with branching and plant height being the important factors that make it up. We identified a gene, <i>MtTCP18</i>, encoding a TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR (TCP) transcription factor highly conserved with <i>Arabidopsis</i> gene <i>BRC1</i> (BRANCHED1) in <i>Medicago truncatula</i>. Sequence analysis revealed that <i>MtTCP18</i> included a conserved basic helix–loop–helix (BHLH) motif and R domain. Expression analysis showed that <i>MtTCP18</i> was expressed in all organs examined, with relatively higher expression in pods and axillary buds. Subcellular localization analysis showed that MtTCP18 was localized in the nucleus and exhibited transcriptional activation activity. These results supported its role as a transcription factor. Meanwhile, we identified a homozygous mutant line (NF14875) with a mutation caused by Tnt1 insertion into <i>MtTCP18</i>. Mutant analysis showed that the mutation of <i>MtTCP18</i> altered plant structure, with increased plant height and branch number. Moreover, we found that the expression of auxin early response genes was modulated in the mutant. Therefore, <i>MtTCP18</i> may be a promising candidate gene for breeders to optimize plant structure for crop improvement.https://www.mdpi.com/2223-7747/13/7/1012TCP transcription factorsplant structureTnt1 mutantauxin<i>Medicago truncatula</i> |
| spellingShingle | Xiaoyue Su Junzan Zheng Xiaoxuan Diao Zhongyi Yang Deyue Yu Fang Huang <i>MtTCP18</i> Regulates Plant Structure in <i>Medicago truncatula</i> Plants TCP transcription factors plant structure Tnt1 mutant auxin <i>Medicago truncatula</i> |
| title | <i>MtTCP18</i> Regulates Plant Structure in <i>Medicago truncatula</i> |
| title_full | <i>MtTCP18</i> Regulates Plant Structure in <i>Medicago truncatula</i> |
| title_fullStr | <i>MtTCP18</i> Regulates Plant Structure in <i>Medicago truncatula</i> |
| title_full_unstemmed | <i>MtTCP18</i> Regulates Plant Structure in <i>Medicago truncatula</i> |
| title_short | <i>MtTCP18</i> Regulates Plant Structure in <i>Medicago truncatula</i> |
| title_sort | i mttcp18 i regulates plant structure in i medicago truncatula i |
| topic | TCP transcription factors plant structure Tnt1 mutant auxin <i>Medicago truncatula</i> |
| url | https://www.mdpi.com/2223-7747/13/7/1012 |
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