FUNCTIONAL ACTIVITY OF MURINE B CELL: A ROLE OF MICROENVIRONMENT
Abstract. To study influence of microenvironment upon functional activity of B cells, we used experimental models of adoptive cell transfer from CBA to congenic CBA/N mice lacking CD5+ B-1 cells, and cocultivation of CBA/N splenocytes with spleen, or peritoneal CBA cells. B cell activity was determi...
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2014-07-01
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| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/80 |
| _version_ | 1826534358765273088 |
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| author | I. N. Dyakov I. V. Grigoriev E. V. Sidorova I. N. Chernyshova |
| author_facet | I. N. Dyakov I. V. Grigoriev E. V. Sidorova I. N. Chernyshova |
| author_sort | I. N. Dyakov |
| collection | DOAJ |
| description | Abstract. To study influence of microenvironment upon functional activity of B cells, we used experimental models of adoptive cell transfer from CBA to congenic CBA/N mice lacking CD5+ B-1 cells, and cocultivation of CBA/N splenocytes with spleen, or peritoneal CBA cells. B cell activity was determined as numbers of IgM-producing cells, and as amounts of cells producing antibodies to a T-independent antigen type 2 (polyvinylpirrolidone). In vivo distribution of transferred cells was determined as the numbers of cells stained with a vital dye (CDFA-SE) in spleen and peritoneum of recipients. Intravenous injection of CBA splenocytes resulted into a significant (3- to 4-fold) increase in numbers of IgM-producing cells in the spleens of xid–recipients, where their levels reached those of CBA mice. Intravenous injection of CBA/N splenocytes into xid-mice did not induce any increase of IgM-producers in their spleen. That means that increased number of IgM-producers in recipient spleen is due to donor cells, presumably, CD5+ B-1 lymphocytes. Meanwhile, restoration of immune response to polyvinylpirrolidone in xid-mice following transfer of CBA splenocytes was not successful. IgM-producing cells were undetectable in peritoneum of intact mice (both CBA and CBA/N). Intraperitoneal transfer of CBA splenocytes also did not induce their accumulation. It could mean that peritoneal microenvironment inhibits B cell activity. Meanwhile, intravenous injection of «silent» peritoneal cells into CBA/N mice brought about great increase of IgM-producers in recipient spleen, i.e., the «job» of B cells was permitted in other microenvironment. The results yielded in vivo are in agreement with data of in vitro experiments. Addition of CBA splenocytes or peritoneal cells (10-50%) to CBA/N splenocytes induced sharp increase of IgM-producing cells in the cultures. The data obtained provide evidence for a decisive role of microenvironment in functional activity of murine B lymphocytes. |
| first_indexed | 2024-03-08T05:52:11Z |
| format | Article |
| id | doaj.art-f832b58a852c4da5ab66211623b4a4f7 |
| institution | Directory Open Access Journal |
| issn | 1563-0625 2313-741X |
| language | Russian |
| last_indexed | 2025-03-14T02:21:45Z |
| publishDate | 2014-07-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj.art-f832b58a852c4da5ab66211623b4a4f72025-03-11T17:59:00ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2014-07-01101515810.15789/1563-0625-2008-1-51-5878FUNCTIONAL ACTIVITY OF MURINE B CELL: A ROLE OF MICROENVIRONMENTI. N. Dyakov0I. V. Grigoriev1E. V. Sidorova2I. N. Chernyshova3ГУ НИИ вакцин и сывороток им. И.И. Мечникова РАМН, МоскваГУ НИИ вакцин и сывороток им. И.И. Мечникова РАМН, МоскваГУ НИИ вакцин и сывороток им. И.И. Мечникова РАМН, МоскваГУ НИИ вакцин и сывороток им. И.И. Мечникова РАМН, МоскваAbstract. To study influence of microenvironment upon functional activity of B cells, we used experimental models of adoptive cell transfer from CBA to congenic CBA/N mice lacking CD5+ B-1 cells, and cocultivation of CBA/N splenocytes with spleen, or peritoneal CBA cells. B cell activity was determined as numbers of IgM-producing cells, and as amounts of cells producing antibodies to a T-independent antigen type 2 (polyvinylpirrolidone). In vivo distribution of transferred cells was determined as the numbers of cells stained with a vital dye (CDFA-SE) in spleen and peritoneum of recipients. Intravenous injection of CBA splenocytes resulted into a significant (3- to 4-fold) increase in numbers of IgM-producing cells in the spleens of xid–recipients, where their levels reached those of CBA mice. Intravenous injection of CBA/N splenocytes into xid-mice did not induce any increase of IgM-producers in their spleen. That means that increased number of IgM-producers in recipient spleen is due to donor cells, presumably, CD5+ B-1 lymphocytes. Meanwhile, restoration of immune response to polyvinylpirrolidone in xid-mice following transfer of CBA splenocytes was not successful. IgM-producing cells were undetectable in peritoneum of intact mice (both CBA and CBA/N). Intraperitoneal transfer of CBA splenocytes also did not induce their accumulation. It could mean that peritoneal microenvironment inhibits B cell activity. Meanwhile, intravenous injection of «silent» peritoneal cells into CBA/N mice brought about great increase of IgM-producers in recipient spleen, i.e., the «job» of B cells was permitted in other microenvironment. The results yielded in vivo are in agreement with data of in vitro experiments. Addition of CBA splenocytes or peritoneal cells (10-50%) to CBA/N splenocytes induced sharp increase of IgM-producing cells in the cultures. The data obtained provide evidence for a decisive role of microenvironment in functional activity of murine B lymphocytes.https://www.mimmun.ru/mimmun/article/view/80в-lymphocyteslocal microenvironmentimmunoglobulins |
| spellingShingle | I. N. Dyakov I. V. Grigoriev E. V. Sidorova I. N. Chernyshova FUNCTIONAL ACTIVITY OF MURINE B CELL: A ROLE OF MICROENVIRONMENT Медицинская иммунология в-lymphocytes local microenvironment immunoglobulins |
| title | FUNCTIONAL ACTIVITY OF MURINE B CELL: A ROLE OF MICROENVIRONMENT |
| title_full | FUNCTIONAL ACTIVITY OF MURINE B CELL: A ROLE OF MICROENVIRONMENT |
| title_fullStr | FUNCTIONAL ACTIVITY OF MURINE B CELL: A ROLE OF MICROENVIRONMENT |
| title_full_unstemmed | FUNCTIONAL ACTIVITY OF MURINE B CELL: A ROLE OF MICROENVIRONMENT |
| title_short | FUNCTIONAL ACTIVITY OF MURINE B CELL: A ROLE OF MICROENVIRONMENT |
| title_sort | functional activity of murine b cell a role of microenvironment |
| topic | в-lymphocytes local microenvironment immunoglobulins |
| url | https://www.mimmun.ru/mimmun/article/view/80 |
| work_keys_str_mv | AT indyakov functionalactivityofmurinebcellaroleofmicroenvironment AT ivgrigoriev functionalactivityofmurinebcellaroleofmicroenvironment AT evsidorova functionalactivityofmurinebcellaroleofmicroenvironment AT inchernyshova functionalactivityofmurinebcellaroleofmicroenvironment |