Profiling Anti-Apoptotic BCL-xL Protein Expression in Glioblastoma Tumorspheres
Glioblastoma (GBM) is one of the cancers with the worst prognosis, despite huge efforts to understand its unusual heterogeneity and aggressiveness. This is mainly due to glioblastoma stem cells (GSCs), which are also responsible for the frequent tumor recurrence following surgery, chemotherapy or ra...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/12/10/2853 |
| _version_ | 1797551974558728192 |
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| author | Deborah Fanfone Ahmed Idbaih Jade Mammi Mathieu Gabut Gabriel Ichim |
| author_facet | Deborah Fanfone Ahmed Idbaih Jade Mammi Mathieu Gabut Gabriel Ichim |
| author_sort | Deborah Fanfone |
| collection | DOAJ |
| description | Glioblastoma (GBM) is one of the cancers with the worst prognosis, despite huge efforts to understand its unusual heterogeneity and aggressiveness. This is mainly due to glioblastoma stem cells (GSCs), which are also responsible for the frequent tumor recurrence following surgery, chemotherapy or radiotherapy. In this study, we investigate the expression pattern of the anti-apoptotic BCL-xL protein in several GBM cell lines and the role it might play in GSC-enriched tumorspheres. We report that several GBM cell lines have an increased BCL-xL expression in tumorspheres compared to differentiated cells. Moreover, by artificially modulating BCL-xL expression, we unravel a correlation between BCL-xL and tumorsphere size. In addition, BCL-xL upregulation appears to sensitize GBM tumorspheres to newly developed BH3 mimetics, opening promising therapeutic perspectives for treating GBM patients. |
| first_indexed | 2024-03-10T15:53:27Z |
| format | Article |
| id | doaj.art-f833b4f1bd0249b4a69b1a077d9f103f |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T15:53:27Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-f833b4f1bd0249b4a69b1a077d9f103f2023-11-20T15:55:30ZengMDPI AGCancers2072-66942020-10-011210285310.3390/cancers12102853Profiling Anti-Apoptotic BCL-xL Protein Expression in Glioblastoma TumorspheresDeborah Fanfone0Ahmed Idbaih1Jade Mammi2Mathieu Gabut3Gabriel Ichim4Cancer Research Centre of Lyon (CRCL) INSERM 1052, CNRS 5286, 69008 Lyon, FranceSorbonne Université, INSERM, CNRS, UMR S 1127, Institut du Cerveau et de la Moelle épinière, ICM, 75013 Paris, FranceCancer Research Centre of Lyon (CRCL) INSERM 1052, CNRS 5286, 69008 Lyon, FranceCancer Research Centre of Lyon (CRCL) INSERM 1052, CNRS 5286, 69008 Lyon, FranceCancer Research Centre of Lyon (CRCL) INSERM 1052, CNRS 5286, 69008 Lyon, FranceGlioblastoma (GBM) is one of the cancers with the worst prognosis, despite huge efforts to understand its unusual heterogeneity and aggressiveness. This is mainly due to glioblastoma stem cells (GSCs), which are also responsible for the frequent tumor recurrence following surgery, chemotherapy or radiotherapy. In this study, we investigate the expression pattern of the anti-apoptotic BCL-xL protein in several GBM cell lines and the role it might play in GSC-enriched tumorspheres. We report that several GBM cell lines have an increased BCL-xL expression in tumorspheres compared to differentiated cells. Moreover, by artificially modulating BCL-xL expression, we unravel a correlation between BCL-xL and tumorsphere size. In addition, BCL-xL upregulation appears to sensitize GBM tumorspheres to newly developed BH3 mimetics, opening promising therapeutic perspectives for treating GBM patients.https://www.mdpi.com/2072-6694/12/10/2853glioblastomacancer stem cellsBCL-xLapoptosisBH3 mimeticstherapeutic opportunity |
| spellingShingle | Deborah Fanfone Ahmed Idbaih Jade Mammi Mathieu Gabut Gabriel Ichim Profiling Anti-Apoptotic BCL-xL Protein Expression in Glioblastoma Tumorspheres Cancers glioblastoma cancer stem cells BCL-xL apoptosis BH3 mimetics therapeutic opportunity |
| title | Profiling Anti-Apoptotic BCL-xL Protein Expression in Glioblastoma Tumorspheres |
| title_full | Profiling Anti-Apoptotic BCL-xL Protein Expression in Glioblastoma Tumorspheres |
| title_fullStr | Profiling Anti-Apoptotic BCL-xL Protein Expression in Glioblastoma Tumorspheres |
| title_full_unstemmed | Profiling Anti-Apoptotic BCL-xL Protein Expression in Glioblastoma Tumorspheres |
| title_short | Profiling Anti-Apoptotic BCL-xL Protein Expression in Glioblastoma Tumorspheres |
| title_sort | profiling anti apoptotic bcl xl protein expression in glioblastoma tumorspheres |
| topic | glioblastoma cancer stem cells BCL-xL apoptosis BH3 mimetics therapeutic opportunity |
| url | https://www.mdpi.com/2072-6694/12/10/2853 |
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