Targeted Delivery of Mannosylated Nanoparticles Improve Prophylactic Efficacy of Immersion Vaccine against Fish Viral Disease
Immersion vaccination is considered as the most effective method for juvenile fish in preventing viral disease, due to its convenience for mass vaccination and stress-free administration. However, immune responses following immersion vaccination are generally less robust and of shorter duration than...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-02-01
|
| Series: | Vaccines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-393X/8/1/87 |
| _version_ | 1828119156277379072 |
|---|---|
| author | Bin Zhu Chen Zhang Zhao Zhao Gao-Xue Wang |
| author_facet | Bin Zhu Chen Zhang Zhao Zhao Gao-Xue Wang |
| author_sort | Bin Zhu |
| collection | DOAJ |
| description | Immersion vaccination is considered as the most effective method for juvenile fish in preventing viral disease, due to its convenience for mass vaccination and stress-free administration. However, immune responses following immersion vaccination are generally less robust and of shorter duration than those induced through intraperitoneal injection. Herein, to improve the efficacy of the immersion vaccine, we constructed a targeted single-walled carbon nanotubes-based immersion vaccine delivery system (CNTs-M-VP7), the surface of which are modified with mannose to allow antigen-presenting cells’ (APCs) targeting. The targeting ability of CNTs-M-VP7 was confirmed in vivo and in vitro. Critically, this immersion CNTs-M-VP7 vaccine could cross into the fish body through mucosal tissues (skin, gill, and intestine), and then present to immune-related tissues. Moreover, CNTs-M-VP7 could significantly induce the maturation and presenting process of APCs, which would then trigger robust immune responses. Altogether, this study demonstrates that the single-walled carbon nanotubes (SWCNTs)-based targeted nanovaccine delivery system shows the potential to be an effective prophylactic against fish viral disease. |
| first_indexed | 2024-04-11T13:43:35Z |
| format | Article |
| id | doaj.art-f837f806c2914e50a0fde7dac6ac198c |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-04-11T13:43:35Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-f837f806c2914e50a0fde7dac6ac198c2022-12-22T04:21:10ZengMDPI AGVaccines2076-393X2020-02-01818710.3390/vaccines8010087vaccines8010087Targeted Delivery of Mannosylated Nanoparticles Improve Prophylactic Efficacy of Immersion Vaccine against Fish Viral DiseaseBin Zhu0Chen Zhang1Zhao Zhao2Gao-Xue Wang3Department of aquaculture, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaDepartment of aquaculture, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaDepartment of aquaculture, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaDepartment of aquaculture, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaImmersion vaccination is considered as the most effective method for juvenile fish in preventing viral disease, due to its convenience for mass vaccination and stress-free administration. However, immune responses following immersion vaccination are generally less robust and of shorter duration than those induced through intraperitoneal injection. Herein, to improve the efficacy of the immersion vaccine, we constructed a targeted single-walled carbon nanotubes-based immersion vaccine delivery system (CNTs-M-VP7), the surface of which are modified with mannose to allow antigen-presenting cells’ (APCs) targeting. The targeting ability of CNTs-M-VP7 was confirmed in vivo and in vitro. Critically, this immersion CNTs-M-VP7 vaccine could cross into the fish body through mucosal tissues (skin, gill, and intestine), and then present to immune-related tissues. Moreover, CNTs-M-VP7 could significantly induce the maturation and presenting process of APCs, which would then trigger robust immune responses. Altogether, this study demonstrates that the single-walled carbon nanotubes (SWCNTs)-based targeted nanovaccine delivery system shows the potential to be an effective prophylactic against fish viral disease.https://www.mdpi.com/2076-393X/8/1/87targeted nanovaccinemannosecarbon nanotubesviral diseaseimmune response |
| spellingShingle | Bin Zhu Chen Zhang Zhao Zhao Gao-Xue Wang Targeted Delivery of Mannosylated Nanoparticles Improve Prophylactic Efficacy of Immersion Vaccine against Fish Viral Disease Vaccines targeted nanovaccine mannose carbon nanotubes viral disease immune response |
| title | Targeted Delivery of Mannosylated Nanoparticles Improve Prophylactic Efficacy of Immersion Vaccine against Fish Viral Disease |
| title_full | Targeted Delivery of Mannosylated Nanoparticles Improve Prophylactic Efficacy of Immersion Vaccine against Fish Viral Disease |
| title_fullStr | Targeted Delivery of Mannosylated Nanoparticles Improve Prophylactic Efficacy of Immersion Vaccine against Fish Viral Disease |
| title_full_unstemmed | Targeted Delivery of Mannosylated Nanoparticles Improve Prophylactic Efficacy of Immersion Vaccine against Fish Viral Disease |
| title_short | Targeted Delivery of Mannosylated Nanoparticles Improve Prophylactic Efficacy of Immersion Vaccine against Fish Viral Disease |
| title_sort | targeted delivery of mannosylated nanoparticles improve prophylactic efficacy of immersion vaccine against fish viral disease |
| topic | targeted nanovaccine mannose carbon nanotubes viral disease immune response |
| url | https://www.mdpi.com/2076-393X/8/1/87 |
| work_keys_str_mv | AT binzhu targeteddeliveryofmannosylatednanoparticlesimproveprophylacticefficacyofimmersionvaccineagainstfishviraldisease AT chenzhang targeteddeliveryofmannosylatednanoparticlesimproveprophylacticefficacyofimmersionvaccineagainstfishviraldisease AT zhaozhao targeteddeliveryofmannosylatednanoparticlesimproveprophylacticefficacyofimmersionvaccineagainstfishviraldisease AT gaoxuewang targeteddeliveryofmannosylatednanoparticlesimproveprophylacticefficacyofimmersionvaccineagainstfishviraldisease |