Evaluation of dried blood spots with a multiplex assay for measuring recent HIV-1 infection.
Laboratory-based HIV tests for recent infection (TRIs), which primarily measure a specific serological biomarker(s) that distinguishes recent from long-term HIV infection, have facilitated the estimation of population-based incidence. Dried blood spots (DBS) on filter paper are an attractive sample...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4169399?pdf=render |
| _version_ | 1818578642430590976 |
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| author | Kelly A Curtis Krystin M Ambrose M Susan Kennedy S Michele Owen |
| author_facet | Kelly A Curtis Krystin M Ambrose M Susan Kennedy S Michele Owen |
| author_sort | Kelly A Curtis |
| collection | DOAJ |
| description | Laboratory-based HIV tests for recent infection (TRIs), which primarily measure a specific serological biomarker(s) that distinguishes recent from long-term HIV infection, have facilitated the estimation of population-based incidence. Dried blood spots (DBS) on filter paper are an attractive sample source for HIV surveillance, given the simplified and cost-effective methods of specimen collection, storage, and shipment. Here, we evaluated the use of DBS in conjunction with an in-house multiplex TRI, the HIV-1-specific Bio-Plex assay, which measures direct antibody binding and avidity to multiple HIV-1 analytes. The assay performance was comparable between matched plasma and DBS samples from HIV-1 infected individuals obtained from diverse sources. The coefficients of variation, comparing the median antibody reactivity for each analyte between plasma and DBS, ranged from 2.78% to 9.40% and the correlation coefficients between the two sample types ranged from 0.89 to 0.97, depending on the analyte. The correlation in antibody reactivity between laboratory and site-prepared DBS for each analyte ranged from 0.87 to 0.98 and from 0.90 to 0.97 between site-prepared DBS and plasma. The correlation in assay measures between plasma and DBS indicate that the sample types can be used interchangeably with the Bio-Plex format, without negatively impacting the misclassification rate of the assay. |
| first_indexed | 2024-12-16T06:49:03Z |
| format | Article |
| id | doaj.art-f838f511d27d4ef2ac699692814cb61c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T06:49:03Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f838f511d27d4ef2ac699692814cb61c2022-12-21T22:40:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0199e10715310.1371/journal.pone.0107153Evaluation of dried blood spots with a multiplex assay for measuring recent HIV-1 infection.Kelly A CurtisKrystin M AmbroseM Susan KennedyS Michele OwenLaboratory-based HIV tests for recent infection (TRIs), which primarily measure a specific serological biomarker(s) that distinguishes recent from long-term HIV infection, have facilitated the estimation of population-based incidence. Dried blood spots (DBS) on filter paper are an attractive sample source for HIV surveillance, given the simplified and cost-effective methods of specimen collection, storage, and shipment. Here, we evaluated the use of DBS in conjunction with an in-house multiplex TRI, the HIV-1-specific Bio-Plex assay, which measures direct antibody binding and avidity to multiple HIV-1 analytes. The assay performance was comparable between matched plasma and DBS samples from HIV-1 infected individuals obtained from diverse sources. The coefficients of variation, comparing the median antibody reactivity for each analyte between plasma and DBS, ranged from 2.78% to 9.40% and the correlation coefficients between the two sample types ranged from 0.89 to 0.97, depending on the analyte. The correlation in antibody reactivity between laboratory and site-prepared DBS for each analyte ranged from 0.87 to 0.98 and from 0.90 to 0.97 between site-prepared DBS and plasma. The correlation in assay measures between plasma and DBS indicate that the sample types can be used interchangeably with the Bio-Plex format, without negatively impacting the misclassification rate of the assay.http://europepmc.org/articles/PMC4169399?pdf=render |
| spellingShingle | Kelly A Curtis Krystin M Ambrose M Susan Kennedy S Michele Owen Evaluation of dried blood spots with a multiplex assay for measuring recent HIV-1 infection. PLoS ONE |
| title | Evaluation of dried blood spots with a multiplex assay for measuring recent HIV-1 infection. |
| title_full | Evaluation of dried blood spots with a multiplex assay for measuring recent HIV-1 infection. |
| title_fullStr | Evaluation of dried blood spots with a multiplex assay for measuring recent HIV-1 infection. |
| title_full_unstemmed | Evaluation of dried blood spots with a multiplex assay for measuring recent HIV-1 infection. |
| title_short | Evaluation of dried blood spots with a multiplex assay for measuring recent HIV-1 infection. |
| title_sort | evaluation of dried blood spots with a multiplex assay for measuring recent hiv 1 infection |
| url | http://europepmc.org/articles/PMC4169399?pdf=render |
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