Anti-angiogenic and anti-inflammatory effects of SERPINA3K on corneal injury.
SERPINA3K is a member of the serine proteinase inhibitor (SERPIN) family. Here we evaluated the therapeutic effects of SERPINA3K on neovascularization and inflammation in a rat cornea alkali burn model that is commonly employed to study corneal wounding. Topical treatment of the injured rat cornea w...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3031620?pdf=render |
| _version_ | 1818851388960014336 |
|---|---|
| author | Xiaochen Liu Zhirong Lin Tong Zhou Ronrong Zong Hui He Zhen Liu Jian-xing Ma Zuguo Liu Yueping Zhou |
| author_facet | Xiaochen Liu Zhirong Lin Tong Zhou Ronrong Zong Hui He Zhen Liu Jian-xing Ma Zuguo Liu Yueping Zhou |
| author_sort | Xiaochen Liu |
| collection | DOAJ |
| description | SERPINA3K is a member of the serine proteinase inhibitor (SERPIN) family. Here we evaluated the therapeutic effects of SERPINA3K on neovascularization and inflammation in a rat cornea alkali burn model that is commonly employed to study corneal wounding. Topical treatment of the injured rat cornea with SERPINA3K (20 µg/eye/day) for 7 days significantly decreased the neovascular area, compared with the groups treated with BSA or PBS. The SERPINA3K treatment also ameliorated the corneal inflammation as evaluated by the inflammatory index. Furthermore, SERPINA3K enhanced the recovery of corneal epithelium after the alkali injury. Toward the mechanism of action, SERPINA3K down-regulated the expression of the pro-angiogenic and pro-inflammatory factors, vascular endothelial growth factor and tumor necrosis factor-α and up-regulated the expression of the anti-angiogenic factor, pigment epithelium-derived factor. SERPINA3K specifically inhibited growth of vascular endothelial cells. Meanwhile, SERPINA3K significantly up-regulated the expression of EGFR in the corneal epithelium. These findings suggest that SERPINA3K has therapeutic potential for corneal inflammation and NV. |
| first_indexed | 2024-12-19T07:04:14Z |
| format | Article |
| id | doaj.art-f83a73b7499c431eb6e7e3ba36c7b7d8 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T07:04:14Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f83a73b7499c431eb6e7e3ba36c7b7d82022-12-21T20:31:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0161e1671210.1371/journal.pone.0016712Anti-angiogenic and anti-inflammatory effects of SERPINA3K on corneal injury.Xiaochen LiuZhirong LinTong ZhouRonrong ZongHui HeZhen LiuJian-xing MaZuguo LiuYueping ZhouSERPINA3K is a member of the serine proteinase inhibitor (SERPIN) family. Here we evaluated the therapeutic effects of SERPINA3K on neovascularization and inflammation in a rat cornea alkali burn model that is commonly employed to study corneal wounding. Topical treatment of the injured rat cornea with SERPINA3K (20 µg/eye/day) for 7 days significantly decreased the neovascular area, compared with the groups treated with BSA or PBS. The SERPINA3K treatment also ameliorated the corneal inflammation as evaluated by the inflammatory index. Furthermore, SERPINA3K enhanced the recovery of corneal epithelium after the alkali injury. Toward the mechanism of action, SERPINA3K down-regulated the expression of the pro-angiogenic and pro-inflammatory factors, vascular endothelial growth factor and tumor necrosis factor-α and up-regulated the expression of the anti-angiogenic factor, pigment epithelium-derived factor. SERPINA3K specifically inhibited growth of vascular endothelial cells. Meanwhile, SERPINA3K significantly up-regulated the expression of EGFR in the corneal epithelium. These findings suggest that SERPINA3K has therapeutic potential for corneal inflammation and NV.http://europepmc.org/articles/PMC3031620?pdf=render |
| spellingShingle | Xiaochen Liu Zhirong Lin Tong Zhou Ronrong Zong Hui He Zhen Liu Jian-xing Ma Zuguo Liu Yueping Zhou Anti-angiogenic and anti-inflammatory effects of SERPINA3K on corneal injury. PLoS ONE |
| title | Anti-angiogenic and anti-inflammatory effects of SERPINA3K on corneal injury. |
| title_full | Anti-angiogenic and anti-inflammatory effects of SERPINA3K on corneal injury. |
| title_fullStr | Anti-angiogenic and anti-inflammatory effects of SERPINA3K on corneal injury. |
| title_full_unstemmed | Anti-angiogenic and anti-inflammatory effects of SERPINA3K on corneal injury. |
| title_short | Anti-angiogenic and anti-inflammatory effects of SERPINA3K on corneal injury. |
| title_sort | anti angiogenic and anti inflammatory effects of serpina3k on corneal injury |
| url | http://europepmc.org/articles/PMC3031620?pdf=render |
| work_keys_str_mv | AT xiaochenliu antiangiogenicandantiinflammatoryeffectsofserpina3koncornealinjury AT zhironglin antiangiogenicandantiinflammatoryeffectsofserpina3koncornealinjury AT tongzhou antiangiogenicandantiinflammatoryeffectsofserpina3koncornealinjury AT ronrongzong antiangiogenicandantiinflammatoryeffectsofserpina3koncornealinjury AT huihe antiangiogenicandantiinflammatoryeffectsofserpina3koncornealinjury AT zhenliu antiangiogenicandantiinflammatoryeffectsofserpina3koncornealinjury AT jianxingma antiangiogenicandantiinflammatoryeffectsofserpina3koncornealinjury AT zuguoliu antiangiogenicandantiinflammatoryeffectsofserpina3koncornealinjury AT yuepingzhou antiangiogenicandantiinflammatoryeffectsofserpina3koncornealinjury |