PVA enema ameliorates DSS-induced acute colitis in mice
Abstract Background Ulcerative colitis (UC) represents a clinically challenging condition characterized by persistent damage to the colonic epithelial mucosa as the principal pathological feature. Polyvinyl alcohol (PVA) solution, primarily composed of glue, is a biodegradable polymer material that...
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | BMC Gastroenterology |
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| Online Access: | https://doi.org/10.1186/s12876-023-03005-w |
| _version_ | 1797636858815971328 |
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| author | Zhiyong Zhang Lingnan Kong Ming Lv Yukuan Yao Li Gao Ruchen Zhou Wenlong Ma Jing Li |
| author_facet | Zhiyong Zhang Lingnan Kong Ming Lv Yukuan Yao Li Gao Ruchen Zhou Wenlong Ma Jing Li |
| author_sort | Zhiyong Zhang |
| collection | DOAJ |
| description | Abstract Background Ulcerative colitis (UC) represents a clinically challenging condition characterized by persistent damage to the colonic epithelial mucosa as the principal pathological feature. Polyvinyl alcohol (PVA) solution, primarily composed of glue, is a biodegradable polymer material that has found utility in the medical field. This research endeavors to investigate the therapeutic potential of PVA water solution in ameliorating UC in mice. Methods UC was induced in 48 C57BL/6 mice by administering 2.5% DSS in their diet for 6 days. Mice were treated with different concentrations of PVA (0.1 mg/ml PVA, 0.3 mg/ml PVA, 1 mg/ml PVA, 3 mg/ml PVA, 10 mg/ml PVA) enemas (n = 6). Disease Activity Index (DAI) and histologic score were evaluated for inflammation degree. Furthermore, mouse colon organoids were cultured, which were used to assess the effects of PVA on expansion in vitro. Results PVA aqueous solutions (1 mg/ml and 3 mg/ml) were able to alleviate the DAI in mice. By DAY 6, there was a significant 3/5-fold decrease in DAI within the 1 mg/ml PVA group (p = 0.02). Histopathology scores demonstrated improvements, while the levels of inflammatory factors in the intestinal mucosal tissue were reduced. Additionally, it was confirmed that PVA could promote the expansion of colonic organoids in vitro. Conclusions In summary, our investigation has yielded findings indicating that PVA holds the potential to ameliorate symptoms associated with colitis in murine subjects afflicted by DSS-induced colitis, primarily through its facilitation of intestinal stem cell expansion. This study might provide a new candidate for the clinical treatment of ulcerative colitis. |
| first_indexed | 2024-03-11T12:41:17Z |
| format | Article |
| id | doaj.art-f83b3c219fa2410dbea575b7d55cbf6d |
| institution | Directory Open Access Journal |
| issn | 1471-230X |
| language | English |
| last_indexed | 2024-03-11T12:41:17Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Gastroenterology |
| spelling | doaj.art-f83b3c219fa2410dbea575b7d55cbf6d2023-11-05T12:19:52ZengBMCBMC Gastroenterology1471-230X2023-10-0123111210.1186/s12876-023-03005-wPVA enema ameliorates DSS-induced acute colitis in miceZhiyong Zhang0Lingnan Kong1Ming Lv2Yukuan Yao3Li Gao4Ruchen Zhou5Wenlong Ma6Jing Li7School of Clinical Medicine, Weifang Medical UniversitySchool of Clinical Medicine, Weifang Medical UniversityZibo Central HospitalSchool of Clinical Medicine, Weifang Medical UniversityDepartment of Outpatient, Zibo Central HospitalDepartment of Gastroenterology, Qilu Hospital, Cheeloo College of Medicine, Shandong UniversityDepartment of Gastroenterology, Qilu Hospital, Cheeloo College of Medicine, Shandong UniversityDepartment of Pathology, Zibo Central HospitalAbstract Background Ulcerative colitis (UC) represents a clinically challenging condition characterized by persistent damage to the colonic epithelial mucosa as the principal pathological feature. Polyvinyl alcohol (PVA) solution, primarily composed of glue, is a biodegradable polymer material that has found utility in the medical field. This research endeavors to investigate the therapeutic potential of PVA water solution in ameliorating UC in mice. Methods UC was induced in 48 C57BL/6 mice by administering 2.5% DSS in their diet for 6 days. Mice were treated with different concentrations of PVA (0.1 mg/ml PVA, 0.3 mg/ml PVA, 1 mg/ml PVA, 3 mg/ml PVA, 10 mg/ml PVA) enemas (n = 6). Disease Activity Index (DAI) and histologic score were evaluated for inflammation degree. Furthermore, mouse colon organoids were cultured, which were used to assess the effects of PVA on expansion in vitro. Results PVA aqueous solutions (1 mg/ml and 3 mg/ml) were able to alleviate the DAI in mice. By DAY 6, there was a significant 3/5-fold decrease in DAI within the 1 mg/ml PVA group (p = 0.02). Histopathology scores demonstrated improvements, while the levels of inflammatory factors in the intestinal mucosal tissue were reduced. Additionally, it was confirmed that PVA could promote the expansion of colonic organoids in vitro. Conclusions In summary, our investigation has yielded findings indicating that PVA holds the potential to ameliorate symptoms associated with colitis in murine subjects afflicted by DSS-induced colitis, primarily through its facilitation of intestinal stem cell expansion. This study might provide a new candidate for the clinical treatment of ulcerative colitis.https://doi.org/10.1186/s12876-023-03005-wColonic mucosal epitheliumEnema therapyOrganoidsPolyvinyl alcoholUlcerative Colitis |
| spellingShingle | Zhiyong Zhang Lingnan Kong Ming Lv Yukuan Yao Li Gao Ruchen Zhou Wenlong Ma Jing Li PVA enema ameliorates DSS-induced acute colitis in mice BMC Gastroenterology Colonic mucosal epithelium Enema therapy Organoids Polyvinyl alcohol Ulcerative Colitis |
| title | PVA enema ameliorates DSS-induced acute colitis in mice |
| title_full | PVA enema ameliorates DSS-induced acute colitis in mice |
| title_fullStr | PVA enema ameliorates DSS-induced acute colitis in mice |
| title_full_unstemmed | PVA enema ameliorates DSS-induced acute colitis in mice |
| title_short | PVA enema ameliorates DSS-induced acute colitis in mice |
| title_sort | pva enema ameliorates dss induced acute colitis in mice |
| topic | Colonic mucosal epithelium Enema therapy Organoids Polyvinyl alcohol Ulcerative Colitis |
| url | https://doi.org/10.1186/s12876-023-03005-w |
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