Quantitative Imaging of Genetically Encoded Fluorescence Lifetime Biosensors
Genetically encoded fluorescence lifetime biosensors have emerged as powerful tools for quantitative imaging, enabling precise measurement of cellular metabolites, molecular interactions, and dynamic cellular processes. This review provides an overview of the principles, applications, and advancemen...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/13/10/939 |
| _version_ | 1827721556705411072 |
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| author | Cong Quang Vu Satoshi Arai |
| author_facet | Cong Quang Vu Satoshi Arai |
| author_sort | Cong Quang Vu |
| collection | DOAJ |
| description | Genetically encoded fluorescence lifetime biosensors have emerged as powerful tools for quantitative imaging, enabling precise measurement of cellular metabolites, molecular interactions, and dynamic cellular processes. This review provides an overview of the principles, applications, and advancements in quantitative imaging with genetically encoded fluorescence lifetime biosensors using fluorescence lifetime imaging microscopy (go-FLIM). We highlighted the distinct advantages of fluorescence lifetime-based measurements, including independence from expression levels, excitation power, and focus drift, resulting in robust and reliable measurements compared to intensity-based approaches. Specifically, we focus on two types of go-FLIM, namely Förster resonance energy transfer (FRET)–FLIM and single-fluorescent protein (FP)-based FLIM biosensors, and discuss their unique characteristics and benefits. This review serves as a valuable resource for researchers interested in leveraging fluorescence lifetime imaging to study molecular interactions and cellular metabolism with high precision and accuracy. |
| first_indexed | 2024-03-10T21:24:04Z |
| format | Article |
| id | doaj.art-f841920f0e064be8a0a088335627f7c8 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T21:24:04Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-f841920f0e064be8a0a088335627f7c82023-11-19T15:51:37ZengMDPI AGBiosensors2079-63742023-10-01131093910.3390/bios13100939Quantitative Imaging of Genetically Encoded Fluorescence Lifetime BiosensorsCong Quang Vu0Satoshi Arai1WPI Nano Life Science Institute, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, JapanWPI Nano Life Science Institute, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, JapanGenetically encoded fluorescence lifetime biosensors have emerged as powerful tools for quantitative imaging, enabling precise measurement of cellular metabolites, molecular interactions, and dynamic cellular processes. This review provides an overview of the principles, applications, and advancements in quantitative imaging with genetically encoded fluorescence lifetime biosensors using fluorescence lifetime imaging microscopy (go-FLIM). We highlighted the distinct advantages of fluorescence lifetime-based measurements, including independence from expression levels, excitation power, and focus drift, resulting in robust and reliable measurements compared to intensity-based approaches. Specifically, we focus on two types of go-FLIM, namely Förster resonance energy transfer (FRET)–FLIM and single-fluorescent protein (FP)-based FLIM biosensors, and discuss their unique characteristics and benefits. This review serves as a valuable resource for researchers interested in leveraging fluorescence lifetime imaging to study molecular interactions and cellular metabolism with high precision and accuracy.https://www.mdpi.com/2079-6374/13/10/939genetically encoded fluorescence lifetime biosensorsFLIMFRET–FLIMfluorescent proteinsquantitative imaging |
| spellingShingle | Cong Quang Vu Satoshi Arai Quantitative Imaging of Genetically Encoded Fluorescence Lifetime Biosensors Biosensors genetically encoded fluorescence lifetime biosensors FLIM FRET–FLIM fluorescent proteins quantitative imaging |
| title | Quantitative Imaging of Genetically Encoded Fluorescence Lifetime Biosensors |
| title_full | Quantitative Imaging of Genetically Encoded Fluorescence Lifetime Biosensors |
| title_fullStr | Quantitative Imaging of Genetically Encoded Fluorescence Lifetime Biosensors |
| title_full_unstemmed | Quantitative Imaging of Genetically Encoded Fluorescence Lifetime Biosensors |
| title_short | Quantitative Imaging of Genetically Encoded Fluorescence Lifetime Biosensors |
| title_sort | quantitative imaging of genetically encoded fluorescence lifetime biosensors |
| topic | genetically encoded fluorescence lifetime biosensors FLIM FRET–FLIM fluorescent proteins quantitative imaging |
| url | https://www.mdpi.com/2079-6374/13/10/939 |
| work_keys_str_mv | AT congquangvu quantitativeimagingofgeneticallyencodedfluorescencelifetimebiosensors AT satoshiarai quantitativeimagingofgeneticallyencodedfluorescencelifetimebiosensors |