Niche Differentiation of Active Methane-Oxidizing Bacteria in Estuarine Mangrove Forest Soils in Taiwan
Mangrove forests are one of the important ecosystems in tropical coasts because of their high primary production, which they sustain by sequestering a substantial amount of CO<sub>2</sub> into plant biomass. These forests often experience various levels of inundation and play an importan...
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MDPI AG
2020-08-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/8/1248 |
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| author | Yo-Jin Shiau Chiao-Wen Lin Yuanfeng Cai Zhongjun Jia Yu-Te Lin Chih-Yu Chiu |
| author_facet | Yo-Jin Shiau Chiao-Wen Lin Yuanfeng Cai Zhongjun Jia Yu-Te Lin Chih-Yu Chiu |
| author_sort | Yo-Jin Shiau |
| collection | DOAJ |
| description | Mangrove forests are one of the important ecosystems in tropical coasts because of their high primary production, which they sustain by sequestering a substantial amount of CO<sub>2</sub> into plant biomass. These forests often experience various levels of inundation and play an important role in CH<sub>4</sub> emissions, but the taxonomy of methanotrophs in these systems remains poorly understood. In this study, DNA-based stable isotope probing showed significant niche differentiation in active aerobic methanotrophs in response to niche differentiation in upstream and downstream mangrove soils of the Tamsui estuary in northwestern Taiwan, in which salinity levels differ between winter and summer. <i>Methylobacter</i> and <i>Methylomicrobium</i>-like Type I methanotrophs dominated methane-oxidizing communities in the field conditions and were significantly <sup>13</sup>C-labeled in both upstream and downstream sites, while <i>Methylobacter</i> were well adapted to high salinity and low temperature. The Type II methanotroph <i>Methylocystis</i> comprised only 10–15% of all the methane oxidizers in the upstream site but less than 5% at the downstream site under field conditions. <sup>13</sup>C-DNA levels in <i>Methylocystis</i> were significantly lower than those in Type I methanotrophs, while phylogenetic analysis further revealed the presence of novel methane oxidizers that are phylogenetically distantly related to Type Ia in fresh and incubated soils at a downstream site. These results suggest that Type I methanotrophs display niche differentiation associated with environmental differences between upstream and downstream mangrove soils. |
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| last_indexed | 2024-03-10T17:19:49Z |
| publishDate | 2020-08-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-f843a976dd004bd9b01a9ddce5c72d822023-11-20T10:24:22ZengMDPI AGMicroorganisms2076-26072020-08-0188124810.3390/microorganisms8081248Niche Differentiation of Active Methane-Oxidizing Bacteria in Estuarine Mangrove Forest Soils in TaiwanYo-Jin Shiau0Chiao-Wen Lin1Yuanfeng Cai2Zhongjun Jia3Yu-Te Lin4Chih-Yu Chiu5Department of Bioenvironmental Systems Engineering, National Taiwan University, Taipei 106, TaiwanBiodiversity Research Center, Academia Sinica, Nangang, Taipei 11529, TaiwanState Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, ChinaState Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, ChinaBiodiversity Research Center, Academia Sinica, Nangang, Taipei 11529, TaiwanBiodiversity Research Center, Academia Sinica, Nangang, Taipei 11529, TaiwanMangrove forests are one of the important ecosystems in tropical coasts because of their high primary production, which they sustain by sequestering a substantial amount of CO<sub>2</sub> into plant biomass. These forests often experience various levels of inundation and play an important role in CH<sub>4</sub> emissions, but the taxonomy of methanotrophs in these systems remains poorly understood. In this study, DNA-based stable isotope probing showed significant niche differentiation in active aerobic methanotrophs in response to niche differentiation in upstream and downstream mangrove soils of the Tamsui estuary in northwestern Taiwan, in which salinity levels differ between winter and summer. <i>Methylobacter</i> and <i>Methylomicrobium</i>-like Type I methanotrophs dominated methane-oxidizing communities in the field conditions and were significantly <sup>13</sup>C-labeled in both upstream and downstream sites, while <i>Methylobacter</i> were well adapted to high salinity and low temperature. The Type II methanotroph <i>Methylocystis</i> comprised only 10–15% of all the methane oxidizers in the upstream site but less than 5% at the downstream site under field conditions. <sup>13</sup>C-DNA levels in <i>Methylocystis</i> were significantly lower than those in Type I methanotrophs, while phylogenetic analysis further revealed the presence of novel methane oxidizers that are phylogenetically distantly related to Type Ia in fresh and incubated soils at a downstream site. These results suggest that Type I methanotrophs display niche differentiation associated with environmental differences between upstream and downstream mangrove soils.https://www.mdpi.com/2076-2607/8/8/1248aerobic methane oxidationmethanotrophscoastal mangrove soilDNA stable isotope probing<i>pmoA</i> gene16S rRNA gene |
| spellingShingle | Yo-Jin Shiau Chiao-Wen Lin Yuanfeng Cai Zhongjun Jia Yu-Te Lin Chih-Yu Chiu Niche Differentiation of Active Methane-Oxidizing Bacteria in Estuarine Mangrove Forest Soils in Taiwan Microorganisms aerobic methane oxidation methanotrophs coastal mangrove soil DNA stable isotope probing <i>pmoA</i> gene 16S rRNA gene |
| title | Niche Differentiation of Active Methane-Oxidizing Bacteria in Estuarine Mangrove Forest Soils in Taiwan |
| title_full | Niche Differentiation of Active Methane-Oxidizing Bacteria in Estuarine Mangrove Forest Soils in Taiwan |
| title_fullStr | Niche Differentiation of Active Methane-Oxidizing Bacteria in Estuarine Mangrove Forest Soils in Taiwan |
| title_full_unstemmed | Niche Differentiation of Active Methane-Oxidizing Bacteria in Estuarine Mangrove Forest Soils in Taiwan |
| title_short | Niche Differentiation of Active Methane-Oxidizing Bacteria in Estuarine Mangrove Forest Soils in Taiwan |
| title_sort | niche differentiation of active methane oxidizing bacteria in estuarine mangrove forest soils in taiwan |
| topic | aerobic methane oxidation methanotrophs coastal mangrove soil DNA stable isotope probing <i>pmoA</i> gene 16S rRNA gene |
| url | https://www.mdpi.com/2076-2607/8/8/1248 |
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