Integrative Strategy of Testing Systems for Identification of Endocrine Disruptors Inducing Metabolic Disorders—An Introduction to the OBERON Project
Exposure to chemical substances that can produce endocrine disrupting effects represents one of the most critical public health threats nowadays. In line with the regulatory framework implemented within the European Union (EU) to reduce the levels of endocrine disruptors (EDs) for consumers, new and...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/8/2988 |
| _version_ | 1797569905552261120 |
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| author | Karine Audouze Denis Sarigiannis Paloma Alonso-Magdalena Celine Brochot Maribel Casas Martine Vrijheid Patrick J. Babin Spyros Karakitsios Xavier Coumoul Robert Barouki |
| author_facet | Karine Audouze Denis Sarigiannis Paloma Alonso-Magdalena Celine Brochot Maribel Casas Martine Vrijheid Patrick J. Babin Spyros Karakitsios Xavier Coumoul Robert Barouki |
| author_sort | Karine Audouze |
| collection | DOAJ |
| description | Exposure to chemical substances that can produce endocrine disrupting effects represents one of the most critical public health threats nowadays. In line with the regulatory framework implemented within the European Union (EU) to reduce the levels of endocrine disruptors (EDs) for consumers, new and effective methods for ED testing are needed. The OBERON project will build an integrated testing strategy (ITS) to detect ED-related metabolic disorders by developing, improving and validating a battery of test systems. It will be based on the concept of an integrated approach for testing and assessment (IATA). OBERON will combine (1) experimental methods (in vitro, e.g., using 2D and 3D human-derived cells and tissues, and in vivo, i.e., using zebrafish at different stages), (2) high throughput omics technologies, (3) epidemiology and human biomonitoring studies and (4) advanced computational models (in silico and systems biology) on functional endpoints related to metabolism. Such interdisciplinary framework will help in deciphering EDs based on a mechanistic understanding of toxicity by providing and making available more effective alternative test methods relevant for human health that are in line with regulatory needs. Data generated in OBERON will also allow the development of novel adverse outcome pathways (AOPs). The assays will be pre-validated in order to select the test systems that will show acceptable performance in terms of relevance for the second step of the validation process, i.e., the inter-laboratory validation as ring tests. Therefore, the aim of the OBERON project is to support the organization for economic co-operation and development (OECD) conceptual framework for testing and assessment of single and/or mixture of EDs by developing specific assays not covered by the current tests, and to propose an IATA for ED-related metabolic disorder detection, which will be submitted to the Joint Research Center (JRC) and OECD community. |
| first_indexed | 2024-03-10T20:16:57Z |
| format | Article |
| id | doaj.art-f84488efab024034a4dc0d8b713d37ca |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T20:16:57Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f84488efab024034a4dc0d8b713d37ca2023-11-19T22:30:20ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-04-01218298810.3390/ijms21082988Integrative Strategy of Testing Systems for Identification of Endocrine Disruptors Inducing Metabolic Disorders—An Introduction to the OBERON ProjectKarine Audouze0Denis Sarigiannis1Paloma Alonso-Magdalena2Celine Brochot3Maribel Casas4Martine Vrijheid5Patrick J. Babin6Spyros Karakitsios7Xavier Coumoul8Robert Barouki9Inserm UMR S-1124, Université de Paris, 75006 Paris, FranceHERACLES Research Center on the Exposome and Health, Aristotle University of Thessaloniki, Center for Interdisciplinary Research and Innovation, 57001 Thessaloniki, GreeceInstituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche (IDiBE), Universidad Miguel Hernández, 03202 Elche, SpainInstitut National de l’Environnement Industriel et des Risques (INERIS), Unité Modèles pour l’Ecotoxicologie et la Toxicologie (METO), Parc ALATA BP2, 60550 Verneuil en Halatte, FranceISGlobal, 08003 Barcelona, SpainISGlobal, 08003 Barcelona, SpainDepartment of Life and Health Sciences, University of Bordeaux, INSERM U1211, MRGM, F-33615 Pessac, FranceEnve.X, 55133 Thessaloniki, GreeceInserm UMR S-1124, Université de Paris, 75006 Paris, FranceInserm UMR S-1124, Université de Paris, 75006 Paris, FranceExposure to chemical substances that can produce endocrine disrupting effects represents one of the most critical public health threats nowadays. In line with the regulatory framework implemented within the European Union (EU) to reduce the levels of endocrine disruptors (EDs) for consumers, new and effective methods for ED testing are needed. The OBERON project will build an integrated testing strategy (ITS) to detect ED-related metabolic disorders by developing, improving and validating a battery of test systems. It will be based on the concept of an integrated approach for testing and assessment (IATA). OBERON will combine (1) experimental methods (in vitro, e.g., using 2D and 3D human-derived cells and tissues, and in vivo, i.e., using zebrafish at different stages), (2) high throughput omics technologies, (3) epidemiology and human biomonitoring studies and (4) advanced computational models (in silico and systems biology) on functional endpoints related to metabolism. Such interdisciplinary framework will help in deciphering EDs based on a mechanistic understanding of toxicity by providing and making available more effective alternative test methods relevant for human health that are in line with regulatory needs. Data generated in OBERON will also allow the development of novel adverse outcome pathways (AOPs). The assays will be pre-validated in order to select the test systems that will show acceptable performance in terms of relevance for the second step of the validation process, i.e., the inter-laboratory validation as ring tests. Therefore, the aim of the OBERON project is to support the organization for economic co-operation and development (OECD) conceptual framework for testing and assessment of single and/or mixture of EDs by developing specific assays not covered by the current tests, and to propose an IATA for ED-related metabolic disorder detection, which will be submitted to the Joint Research Center (JRC) and OECD community.https://www.mdpi.com/1422-0067/21/8/2988endocrine disruptorsmetabolismintegrative approachpredictive toxicologymetabolic disordersobesity |
| spellingShingle | Karine Audouze Denis Sarigiannis Paloma Alonso-Magdalena Celine Brochot Maribel Casas Martine Vrijheid Patrick J. Babin Spyros Karakitsios Xavier Coumoul Robert Barouki Integrative Strategy of Testing Systems for Identification of Endocrine Disruptors Inducing Metabolic Disorders—An Introduction to the OBERON Project International Journal of Molecular Sciences endocrine disruptors metabolism integrative approach predictive toxicology metabolic disorders obesity |
| title | Integrative Strategy of Testing Systems for Identification of Endocrine Disruptors Inducing Metabolic Disorders—An Introduction to the OBERON Project |
| title_full | Integrative Strategy of Testing Systems for Identification of Endocrine Disruptors Inducing Metabolic Disorders—An Introduction to the OBERON Project |
| title_fullStr | Integrative Strategy of Testing Systems for Identification of Endocrine Disruptors Inducing Metabolic Disorders—An Introduction to the OBERON Project |
| title_full_unstemmed | Integrative Strategy of Testing Systems for Identification of Endocrine Disruptors Inducing Metabolic Disorders—An Introduction to the OBERON Project |
| title_short | Integrative Strategy of Testing Systems for Identification of Endocrine Disruptors Inducing Metabolic Disorders—An Introduction to the OBERON Project |
| title_sort | integrative strategy of testing systems for identification of endocrine disruptors inducing metabolic disorders an introduction to the oberon project |
| topic | endocrine disruptors metabolism integrative approach predictive toxicology metabolic disorders obesity |
| url | https://www.mdpi.com/1422-0067/21/8/2988 |
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